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4 hits found for Replication protein A 70 kDa DNA-binding subunit

...ASDPZ3 – ...A1-239 and RPAΔ32NΔ70N complex engaged on 3’ss-ds DNA junction NER substrate

DNA repair protein complementing XP-A cellsReplication protein A 70 kDa DNA-binding subunitReplication protein A 32 kDa subunitReplication protein A 14 kDa subunit3-prime ss-ds DNA junction NER model substrate experimental SAS data
MES-FOXS model
Sample: ...A repair protein complementing XP-A cells monomer, 27 kDa ...apiens protein
Replication protein A 70 kDa DNA-binding subunit monomer, 49 kDa ...apiens protein
Replication protein A 32 kDa subunit monomer, 25 kDa ...apiens protein
Replication protein A 14 kDa subunit monomer, 14 kDa ...apiens protein
...A junction NER model substrate monomer, 17 kDa ...A
Buffer: ...aCl, 2% glycerol, 1 mM DTT, pH: 8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...ar 4
...action surfaces between XPA and RPA organize the preincision complex in nucleotide excision repair ...ational Academy of Sciences 119(34) (2022)
...a A, Gallagher K, Jeong E, Topolska-Wós A, Ogorodnik Le Meur K, Tsai C, Tsai M, Kee M, Tainer J, Yeo J, Chazin W, Schärer O
RgGuinier 4.3 nm
Dmax 14.7 nm
VolumePorod 189 nm3

...ASDP24 – ...A1-239 and RPAΔ32NΔ70N complex engaged on 5’ss-ds DNA junction NER substrate

Replication protein A 14 kDa subunitDNA repair protein complementing XP-A cellsReplication protein A 70 kDa DNA-binding subunitReplication protein A 32 kDa subunit5-prime ss-ds DNA junction NER model substrate experimental SAS data
MES-FOXS model
Sample: Replication protein A 14 kDa subunit monomer, 14 kDa ...apiens protein
...A repair protein complementing XP-A cells monomer, 27 kDa ...apiens protein
Replication protein A 70 kDa DNA-binding subunit monomer, 49 kDa ...apiens protein
Replication protein A 32 kDa subunit monomer, 25 kDa ...apiens protein
...A junction NER model substrate monomer, 17 kDa ...A
Buffer: ...aCl, 2% glycerol, 1 mM DTT, pH: 8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...ar 4
...action surfaces between XPA and RPA organize the preincision complex in nucleotide excision repair ...ational Academy of Sciences 119(34) (2022)
...a A, Gallagher K, Jeong E, Topolska-Wós A, Ogorodnik Le Meur K, Tsai C, Tsai M, Kee M, Tainer J, Yeo J, Chazin W, Schärer O
RgGuinier 4.6 nm
Dmax 16.5 nm
VolumePorod 220 nm3

...ASDH44 – ...A-DBD and RPA70AB

DNA repair protein complementing XP-A cellsReplication protein A 70 kDa DNA-binding subunit3-prime Nucleotide Excision Repair Junction Model Substrate experimental SAS data
HADDOCK model
Sample: ...A repair protein complementing XP-A cells monomer, 17 kDa ...apiens protein
Replication protein A 70 kDa DNA-binding subunit monomer, 27 kDa ...apiens protein
...air Junction Model Substrate monomer, 11 kDa ...A
Buffer: ...aCl, 2% glycerol, 1 mM DTT, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 17 Nov 2
A key interaction with RPA orients XPA in NER complexes. ...Acids Res (2020)
...a-Woś AM, Sugitani N, Cordoba JJ, Le Meur KV, Le Meur RA, Kim HS, Yeo JE, Rosenberg D, Hammel M, Schärer OD, Chazin WJ
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 103 nm3

...ASDH54 – ...A-DBD with RPA70AB

DNA repair protein complementing XP-A cellsReplication protein A 70 kDa DNA-binding subunit5-prime Nucleotide Excision Repair Junction Model Substrate experimental SAS data
HADDOCK model
Sample: ...A repair protein complementing XP-A cells monomer, 17 kDa ...apiens protein
Replication protein A 70 kDa DNA-binding subunit monomer, 27 kDa ...apiens protein
...air Junction Model Substrate monomer, 11 kDa ...A
Buffer: ...aCl, 2% glycerol, 1 mM DTT, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 4
A key interaction with RPA orients XPA in NER complexes. ...Acids Res (2020)
...a-Woś AM, Sugitani N, Cordoba JJ, Le Meur KV, Le Meur RA, Kim HS, Yeo JE, Rosenberg D, Hammel M, Schärer OD, Chazin WJ
RgGuinier 2.9 nm
Dmax 97.0 nm
VolumePorod 87 nm3