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2 hits found for S-adenosylmethionine synthase

SASDQ95 – ...S-adenosylmethionine synthase and wild-type S-adenosyl-L-methionine lyase from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Jun 17
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.5 nm
VolumePorod 325 nm3

SASDQA5 – ...S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase (E68Q/Q94A) experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase (E68Q/Q94A) tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 May 7
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 326 nm3