| MWexperimental | 76 | kDa |
| MWexpected | 80 | kDa |
| VPorod | 143 | nm3 |
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log I(s)
6.34×10-2
6.34×10-3
6.34×10-4
6.34×10-5
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s, nm-1
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X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization.
Cavini IA, Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC, Acta Crystallogr D Struct Biol (2023) Europe PMCSASDR79 – MBP-, SUMO-fused heterodimeric SEPT14-SEPT7 septin coiled coil (MBP-SEPT14c+SUMO-SEPT7c)
Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-14 (C-terminal domain of SEPT14 fused to MBP)
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Fits and models
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Synchrotron SAXS data from solutions of MBP-SEPT14c+SUMO-SEPT7c in 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Eiger 4M detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.09464 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 5 mg/ml was injected at a 0.08 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 15°C. 600 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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