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| Sample: |
Thioredoxin 1 monomer, 12 kDa Escherichia coli protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
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| Buffer: |
...10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
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| Experiment: |
SAXS
data collected at ...12, PETRA III on ...13 Jun 15
|
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
|
| RgGuinier |
3.2 |
nm |
| Dmax |
13.9 |
nm |
| VolumePorod |
31 |
nm3 |
|
