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5 hits found for Transcription intermediary factor 1-beta

SASDFS3 – ...transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D

Transcription intermediary factor 1-beta experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta dimer, 82 kDa Mus musculus protein
Buffer: 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Aug 10
A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family. J Mol Biol (2019)
Sun Y, Keown JR, Black MM, Raclot C, Demarais N, Trono D, Turelli P, Goldstone DC
RgGuinier 7.0 nm
Dmax 23.2 nm
VolumePorod 232 nm3

SASDFT3 – ...transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D, complexed with the Krab domain of ZFP809 fused to an N-terminal MBP

Transcription intermediary factor 1-betaZinc finger protein 809 N-terminal MBP fusion experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta dimer, 82 kDa Mus musculus protein
Zinc finger protein 809 N-terminal MBP fusion monomer, 52 kDa Mus musculus protein
Buffer: 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Aug 10
A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family. J Mol Biol (2019)
Sun Y, Keown JR, Black MM, Raclot C, Demarais N, Trono D, Turelli P, Goldstone DC
RgGuinier 6.4 nm
Dmax 22.0 nm
VolumePorod 252 nm3

SASDEV6 – KRAB-associated protein 1 (KAP1); TRIM28; full length protein

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 dimer, 183 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6
KAP1 is an antiparallel dimer with a functional asymmetry. Life Sci Alliance 2(4) (2019)
Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
RgGuinier 9.0 nm
Dmax 38.0 nm
VolumePorod 710 nm3

SASDER7 – KRAB-associated protein 1 (KAP1); TRIM28, amino acids 23-812

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 experimental SAS data
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 Kratky plot
Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 dimer, 175 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6
KAP1 is an antiparallel dimer with a functional asymmetry. Life Sci Alliance 2(4) (2019)
Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
RgGuinier 8.9 nm
Dmax 38.0 nm
VolumePorod 853 nm3

SASDEW6 – KRAB-associated protein 1, (KAP1); TRIM28; 23-418 RBCC domain

Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain dimer, 92 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6
KAP1 is an antiparallel dimer with a functional asymmetry. Life Sci Alliance 2(4) (2019)
Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
RgGuinier 8.3 nm
Dmax 37.0 nm
VolumePorod 381 nm3