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6 hits found for Ubiquitin carboxyl-terminal hydrolase MINDY-2

SASDJ93 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type apo-form

Ubiquitin carboxyl-terminal hydrolase MINDY-2 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
...ubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 45 nm3

SASDJA3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type bound to mono-ubiquitin

Ubiquitin carboxyl-terminal hydrolase MINDY-2Polyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein
...ubiquitin-C monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
...ubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.3 nm
Dmax 6.8 nm
VolumePorod 58 nm3

SASDJB3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to di-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
...ubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
...ubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.5 nm
Dmax 8.0 nm
VolumePorod 68 nm3

SASDJC3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tri-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
...ubiquitin-C trimer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
...ubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.8 nm
Dmax 8.8 nm
VolumePorod 97 nm3

SASDJD3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tetra-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
...ubiquitin-C tetramer, 34 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
...ubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.9 nm
Dmax 9.2 nm
VolumePorod 111 nm3

SASDJE3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to penta-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
...ubiquitin-C pentamer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
...ubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
Abdul Rehman SA, Armstrong LA, Lange SM, Kristariyanto YA, Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.8 nm
Dmax 9.0 nm
VolumePorod 116 nm3