Small Angle Scattering Biological Data Bank SASBDB


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7 hits found for Uricase

SASDHD2 – ...Uricase) from Aspergillus flavus, measured by SEC-SAXS

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data

Sample:	...Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer:	20 mM Tris. 150 mM NaCl, 1 mM EDTA, 5 mM DTT, pH: 8
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2019 Jul 1

...Uricase) from Aspergillus flavus
Tsutomu Matsui

R_g^Guinier	3.3	nm
D_max	9.3	nm
Volume^Porod	225	nm³

SASDUB4 – Urate Oxidase Updated Consensus SAXS Data

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4

Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A

R_g^Guinier	3.2	nm
D_max	9.3	nm

SASDPQ4 – Consensus SAXS Profile - Urate Oxidase

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...

R_g^Guinier	3.2	nm
D_max	9.2	nm
Volume^Porod	220	nm³

SASDPX4 – Consensus SANS Profile - Urate oxidase in 100% v/v D2O buffer

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	3.1	nm
D_max	9.3	nm

SASDP45 – Consensus SANS Profile - Urate oxidase in H2O buffer

Sample:	Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24

R_g^Guinier	3.2	nm
D_max	9.1	nm

SASDPH7 – Urate Oxidase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot

Sample:	...Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	3.2	nm
D_max	8.8	nm
Volume^Porod	221	nm³

SASDPJ7 – Urate Oxidase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	...Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer:	100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8