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9 hits found for calcium ions

SASDKR3 – Deuterated calmodulin bound to the HIV-1 MA protein

Calmodulin-1calcium ionsGag-Pol polyprotein experimental SAS data
MONSA model
Sample: Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
calcium ions tetramer, 0 kDa
Gag-Pol polyprotein monomer, 15 kDa Human immunodeficiency virus … protein
Buffer: 50 mM MOPS, 5 mM CaCl2, 2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2010 Jun 23
Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release. Biophys J 103(3):541-549 (2012)
Taylor JE, Chow JYH, Jeffries CM, Kwan AH, Duff AP, Hamilton WA, Trewhella J
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 55 nm3

SASDNG5 – ...calcium measured by paused-flow SEC-SANS

Neurotransmitter-gated ion-channel ligand-binding domain-containing protein experimental SAS data
GROMACS model
Sample: Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.5 mM deuterated n-Dodecyl-B-D-Maltoside, in D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 23
...calcium-binding and modulatory-domain dynamics in a pentameric ligand-gated ion channel. Proc Natl Acad Sci U S A 119(50):e2210669119 (2022)
Lycksell M, Rovšnik U, Hanke A, Martel A, Howard RJ, Lindahl E
RgGuinier 5.2 nm
Dmax 16.8 nm
VolumePorod 530 nm3

SASDNH5 – ...calcium measured by paused-flow SEC-SANS

Neurotransmitter-gated ion-channel ligand-binding domain-containing protein experimental SAS data
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Kratky plot
Sample: Neurotransmitter-gated ion-channel ligand-binding domain-containing protein pentamer, 342 kDa Desulfofustis sp. PB-SRB1 protein
Buffer: 20 mM Tris, 150 mM NaCl, 10 mM EDTA, 0.5 mM deuterated n-Dodecyl-B-D-Maltoside, in D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 23
...calcium-binding and modulatory-domain dynamics in a pentameric ligand-gated ion channel. Proc Natl Acad Sci U S A 119(50):e2210669119 (2022)
Lycksell M, Rovšnik U, Hanke A, Martel A, Howard RJ, Lindahl E
RgGuinier 5.2 nm
Dmax 16.7 nm
VolumePorod 540 nm3

SASDEC7 – ...Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer: 10 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.4 nm
Dmax 8.9 nm
VolumePorod 134 nm3

SASDED7 – ...Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer: 10 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.4 nm
Dmax 8.9 nm
VolumePorod 134 nm3

SASDEE7 – ...Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer: 10 mM Hepes, pH 6.5, 150 mM NaCl, 0.5 mM CaCl2, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.4 nm
Dmax 8.9 nm
VolumePorod 132 nm3

SASDEF7 – ...Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein
Buffer: 10 mM HEPES pH 6.5 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.4 nm
Dmax 8.9 nm
VolumePorod 140 nm3

SASDEG7 – ...Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 octamer, 187 kDa Homo sapiens protein
Buffer: 10 mM Hepes, pH 4.5, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.7 nm
Dmax 10.0 nm
VolumePorod 285 nm3

SASDEH7 – ...Calcium

Transient receptor potential channel mucolipin 2 experimental SAS data
CORAL model
Sample: Transient receptor potential channel mucolipin 2 octamer, 187 kDa Homo sapiens protein
Buffer: 10 mM Hepes, pH 4.5, 150 mM NaCl, 0.5 mM CaCl2, pH: 4.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20
Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain. Structure (2019)
Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
RgGuinier 3.7 nm
Dmax 10.0 nm
VolumePorod 280 nm3