Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: Q5ZXE0

SASDSY6 – Outer membrane protein MIP (LpMIP) apo state

UniProt ID: Q5ZXE0 (21-233) Outer membrane protein MIP

Outer membrane protein MIP experimental SAS data

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding International Journal of Biological Macromolecules :126366 (2023)
Wiedemann C, Whittaker J, Carrillo V, Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U

R_g^Guinier	3.1	nm
D_max	10.0	nm
Volume^Porod	66	nm³

SASDSZ6 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK095

UniProt ID: Q5ZXE0 (21-233) Outer membrane protein MIP

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

R_g^Guinier	3.1	nm
D_max	10.2	nm
Volume^Porod	65	nm³

SASDS27 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK236

UniProt ID: Q5ZXE0 (21-233) Outer membrane protein MIP

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

R_g^Guinier	3.1	nm
D_max	9.4	nm
Volume^Porod	63	nm³

SASDWF4 – Outer membrane protein MIP from Legionella pneumophila (LpMIP) in complex with inhibitor NJS224

UniProt ID: Q5ZXE0 (21-233) Outer membrane protein MIP

UniProt ID: None (None-None) (2S)‐2‐{[(2S)‐1‐[(4‐ fluorophenyl)methanesulfonyl]piperidin‐2‐ yl]formamido}‐4‐methyl‐N‐[(pyridin‐3‐ yl)methyl]pentanamide

Outer membrane protein MIP(2S)‐2‐{[(2S)‐1‐[(4‐ fluorophenyl)methanesulfonyl]piperidin‐2‐ yl]formamido}‐4‐methyl‐N‐[(pyridin‐3‐ yl)methyl]pentanamide experimental SAS data

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein (2S)‐2‐{[(2S)‐1‐[(4‐ fluorophenyl)methanesulfonyl]piperidin‐2‐ yl]formamido}‐4‐methyl‐N‐[(pyridin‐3‐ yl)methyl]pentanamide monomer, 1 kDa
Buffer:	20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22

Structure and Dynamics of Macrophage Infectivity Potentiator Proteins from Pathogenic Bacteria and Protozoans Bound to Fluorinated Pipecolic Acid Inhibitors. J Med Chem (2025)
Pérez Carrillo VH, Whittaker JJ, Wiedemann C, Harder JM, Lohr T, Jamithireddy AK, Dajka M, Goretzki B, Joseph B, Guskov A, Harmer NJ, Holzgrabe U, Hellmich UA

R_g^Guinier	2.9	nm
D_max	9.7	nm
Volume^Porod	61	nm³

SASDWG4 – Outer membrane protein MIP from Legionella pneumophila (LpMIP) in complex with inhibitor NJS227

UniProt ID: Q5ZXE0 (21-233) Outer membrane protein MIP

UniProt ID: None (None-None) (2S)‐3‐(4‐fluorophenyl)‐2‐{[(2S)‐1‐[(4‐ fluorophenyl)methanesulfonyl]piperidin‐2‐ yl]formamido}‐N‐[(pyridin‐3‐yl)methyl]propanamide

Outer membrane protein MIP(2S)‐3‐(4‐fluorophenyl)‐2‐{[(2S)‐1‐[(4‐ fluorophenyl)methanesulfonyl]piperidin‐2‐ yl]formamido}‐N‐[(pyridin‐3‐yl)methyl]propanamide experimental SAS data

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein (2S)‐3‐(4‐fluorophenyl)‐2‐{[(2S)‐1‐[(4‐ fluorophenyl)methanesulfonyl]piperidin‐2‐ yl]formamido}‐N‐[(pyridin‐3‐yl)methyl]propanamide monomer, 1 kDa
Buffer:	20 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22

R_g^Guinier	3.0	nm
D_max	10.0	nm
Volume^Porod	65	nm³