Browse by DISSEMINATION: Benchmark (standard proteins)

SASDPH7 – Urate Oxidase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 8.8 nm
VolumePorod 221 nm3

SASDPG7 – Ribonuclease A SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 16 nm3

SASDPF7 – Ribonuclease A SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16
Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 16 nm3

SASDMV3 – Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm

Nominal 100 nm diameter polystyrene spheres experimental SAS data
BODIES model
Sample: Nominal 100 nm diameter polystyrene spheres monomer, 315312 kDa
Buffer: Water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 16
Standard polystyrene nanospheres (concentration series data)
Dima Molodenskiy
RgGuinier 33.0 nm
Dmax 100.0 nm
VolumePorod 520 nm3

SASDMW3 – Thermo Fisher polystyrene nanospheres with a diameter of 20 nm

Nominal 20 nm diameter polystyrene spheres experimental SAS data
BODIES model
Sample: Nominal 20 nm diameter polystyrene spheres 0, 8 kDa
Buffer: Water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 16
Standard polystyrene nanospheres (concentration series data)
Dima Molodenskiy
RgGuinier 8.5 nm
Dmax 25.0 nm
VolumePorod 3 nm3

SASDCQ8 – Bovine serum albumin (BSA)

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.8 nm

SASDCR8 – Glucose isomerase

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 100 mM HEPES, 1 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.7 nm

SASDCS8 – Cytochrome C

Cytochrome C experimental SAS data
Cytochrome C Kratky plot
Sample: Cytochrome C monomer, 12 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 1.3 nm

SASDCD8 – Horse spleen apoferritin (WAXS)

Horse spleen apoferritin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Horse spleen apoferritin 24-mer, 476 kDa Equus caballus protein
Buffer: tbs, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 15
WAXS benchmark on standard proteins
Maxim Petoukhov
RgGuinier 6.3 nm

SASDCE8 – Beta amylase from sweet potato (WAXS)

Beta-amylase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
Buffer: tbs, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 15
WAXS benchmark on standard proteins
Maxim Petoukhov
RgGuinier 4.0 nm