Small Angle Scattering Biological Data Bank SASBDB

Browse by MODEL: Ab initio only

SASDAS5 – ad11 Fab

Sample:	AD11 Fab dimer, 50 kDa Mus musculus protein
Buffer:	50 mM Na-phosphate 1mM ethylenediaminetetraacetic aci, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Oct 20

Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody. J Mol Biol 381(4):881-96 (2008)
Covaceuszach S, Cassetta A, Konarev PV, Gonfloni S, Rudolph R, Svergun DI, Lamba D, Cattaneo A

R_g^Guinier	2.6	nm
D_max	8.0	nm
Volume^Porod	95	nm³

SASDAT5 – NGF

Sample:	NGF dimer, 50 kDa Mus musculus protein
Buffer:	10 mM Na-phosphate 150 mM NaCl, pH: 7.8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Oct 20

R_g^Guinier	3.1	nm
D_max	12.0	nm
Volume^Porod	102	nm³

SASDAU5 – ad11 Fab + NGF

Sample:	AD11 Fab dimer, 50 kDa Mus musculus protein NGF dimer, 50 kDa Mus musculus protein
Buffer:	30 mM Na-phosphate 75 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Dec 20

R_g^Guinier	4.3	nm
D_max	16.0	nm
Volume^Porod	255	nm³

SASDLZ5 – Hepatocyte growth factor/Hepatocyte growth factor receptor (NK1-Met567) complex

Hepatocyte growth factorHepatocyte growth factor receptor experimental SAS data

Sample:	Hepatocyte growth factor dimer, 39 kDa Homo sapiens protein Hepatocyte growth factor receptor dimer, 122 kDa Homo sapiens protein
Buffer:	Phosphate-buffered saline containing EDTA, surfactant P20 and 5 M NaCl, pH: 6
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Jun 26

Engineering the NK1 Fragment of Hepatocyte Growth Factor/Scatter Factor as a MET Receptor Antagonist Journal of Molecular Biology 377(3):616-622 (2008)
Youles M, Holmes O, Petoukhov M, Nessen M, Stivala S, Svergun D, Gherardi E

R_g^Guinier	5.3	nm
D_max	20.0	nm
Volume^Porod	290	nm³

SASDNP2 – Experimental SAXS data for hemoglobin conjucted with six-seven copies of PEG dimer (Hb5)

Human hemoglobin conjugated with six-seven copies of 5-kDa PEG experimental SAS data

Sample:	Human hemoglobin conjugated with six-seven copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic Biophysical Journal 94(1):173-181 (2008)
Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R

R_g^Guinier	3.3	nm
D_max	13.0	nm

SASDNR2 – Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2)

Human hemoglobin conjugated with two copies of 5-kDa PEG experimental SAS data

Sample:	Human hemoglobin conjugated with two copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier	2.8	nm
D_max	13.0	nm

SASDMB7 – Glucosamine-6-phosphate Synthase from Candida albicans

N-acetylglucosamine kinase 1 experimental SAS data

Sample:	N-acetylglucosamine kinase 1 tetramer, 219 kDa Candida albicans (strain … protein
Buffer:	0.2 M magnesium acetate, 0.1 M sodium cacodylate, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Apr 28

The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans. J Mol Biol 372(3):672-88 (2007)
Raczynska J, Olchowy J, Konariev PV, Svergun DI, Milewski S, Rypniewski W

R_g^Guinier	5.1	nm
D_max	16.0	nm
Volume^Porod	421	nm³

SASDA98 – Thyroglobulin in Tris/HCl

Sample:	Thyroglobulin dimer, 606 kDa Bos taurus protein
Buffer:	100 mM Tris/HCl 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 19

Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering Journal of Applied Crystallography 40(s1):s245-s249 (2007)
Mylonas E, Svergun D

R_g^Guinier	7.9	nm
D_max	24.0	nm

SASDMA7 – Prion protein aggregate in solution

Major prion protein experimental SAS data

Sample:	Major prion protein 24-mer, 664 kDa Homo sapiens protein
Buffer:	5 mM sodium acetate, pH: 5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jun 2

Structural characterization of beta-sheeted oligomers formed on the pathway of oxidative prion protein aggregation in vitro. J Struct Biol 157(2):308-20 (2007)
Redecke L, von Bergen M, Clos J, Konarev PV, Svergun DI, Fittschen UE, Broekaert JA, Bruns O, Georgieva D, Mandelkow E, Genov N, Betzel C

R_g^Guinier	9.8	nm
D_max	32.0	nm
Volume^Porod	3320	nm³

SASDAQ5 – Lumazine Synthase

Sample:	Lumazine Synthase, 960 kDa Bacillus subtilis protein
Buffer:	Borate buffer, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Nov 25

Multiple assembly states of lumazine synthase: a model relating catalytic function and molecular assembly. J Mol Biol 362(4):753-70 (2006)
Zhang X, Konarev PV, Petoukhov MV, Svergun DI, Xing L, Cheng RH, Haase I, Fischer M, Bacher A, Ladenstein R, Meining W

R_g^Guinier	6.2	nm
D_max	15.5	nm
Volume^Porod	1450	nm³

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