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50 hits found for Alam

SASDTZ9 – HIV-1 Envelope Glycoprotein SOSIP from a CH505 isolate 53 weeks post-infection at 25 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.4 nm
Dmax 16.7 nm
VolumePorod 768 nm3

SASDU22 – HIV-1 Envelope Glycoprotein SOSIP from a CH505 isolate 78 weeks post-infection at 25 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.1 nm
Dmax 16.0 nm
VolumePorod 709 nm3

SASDU32 – HIV-1 Envelope Glycoprotein SOSIP from a CH505 isolate 100 weeks post-infection at 25 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.2 nm
Dmax 17.2 nm
VolumePorod 770 nm3

SASDU42 – HIV-1 Envelope Glycoprotein SOSIP from a BG505 isolate containing T332N mutation at 25 °C

BG505SOSIP.664T332N Env glycoprotein experimental SAS data
BG505SOSIP.664T332N Env glycoprotein Kratky plot
Sample: BG505SOSIP.664T332N Env glycoprotein trimer, 213 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.0 nm
Dmax 20.7 nm
VolumePorod 712 nm3

SASDJS5 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-monomer

Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial monomer, 70 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Expression and analysis of the SAM-dependent RNA methyltransferase Rsm22 from Saccharomyces cerevisiae Acta Crystallographica Section D Structural Biology 77(6) (2021)
Alam J, Rahman F, Sah-Teli S, Venkatesan R, Koski M, Autio K, Hiltunen J, Kastaniotis A
RgGuinier 3.8 nm
Dmax 13.9 nm
VolumePorod 160 nm3

SASDJT5 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-dimer

dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial dimer, 141 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Expression and analysis of the SAM-dependent RNA methyltransferase Rsm22 from Saccharomyces cerevisiae Acta Crystallographica Section D Structural Biology 77(6) (2021)
Alam J, Rahman F, Sah-Teli S, Venkatesan R, Koski M, Autio K, Hiltunen J, Kastaniotis A
RgGuinier 5.0 nm
Dmax 18.2 nm
VolumePorod 516 nm3

SASDJ26 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-monomer

Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial monomer, 70 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam
RgGuinier 3.8 nm
Dmax 13.9 nm
VolumePorod 160 nm3

SASDJ36 – Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-dimer

dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial dimer, 141 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam
RgGuinier 5.0 nm
Dmax 18.2 nm
VolumePorod 516 nm3

SASDT29 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 25 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.6 nm
Dmax 15.5 nm
VolumePorod 705 nm3

SASDT39 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 35 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.6 nm
Dmax 15.4 nm
VolumePorod 704 nm3

SASDT49 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 44 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 27
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.6 nm
Dmax 15.6 nm
VolumePorod 702 nm3

SASDT59 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 50 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.6 nm
Dmax 15.6 nm
VolumePorod 699 nm3

SASDT69 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1.5 µs post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.6 nm
Dmax 15.5 nm
VolumePorod 706 nm3

SASDT79 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 3 µs post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.6 nm
Dmax 15.5 nm
VolumePorod 706 nm3

SASDT89 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 5 µs post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.6 nm
Dmax 15.5 nm
VolumePorod 706 nm3

SASDH99 – Meiotic localizer of BRCA2 bound to BRME1 - MEILB2a1+2-BRME1 2:2 complex

BRME1Meiotic localizer of BRCA2 experimental SAS data
DAMMIF model
Sample: BRME1 dimer, 23 kDa Mus musculus protein
Meiotic localizer of BRCA2 dimer, 26 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 7
The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells Nature Communications 11(1) (2020)
...Alam I, Livera G, Emmanuelle M, Wang P, Nandakumar J, Davies O, Shibuya H
RgGuinier 5.0 nm
Dmax 19.0 nm
VolumePorod 104 nm3

SASDT99 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 10 µs post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.3 nm
Dmax 15.3 nm
VolumePorod 729 nm3

SASDHA9 – Meiotic localizer of BRCA2 - MEILB2a1+2 dimer

Meiotic localizer of BRCA2 experimental SAS data
DAMMIF model
Sample: Meiotic localizer of BRCA2 dimer, 26 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 7
The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells Nature Communications 11(1) (2020)
...Alam I, Livera G, Emmanuelle M, Wang P, Nandakumar J, Davies O, Shibuya H
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 88 nm3

SASDTA9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 50 µs post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.3 nm
Dmax 15.4 nm
VolumePorod 729 nm3

SASDHB9 – Meiotic localizer of BRCA2 - MEILB2a1+2 octamer

Meiotic localizer of BRCA2 experimental SAS data
DAMMIF model
Sample: Meiotic localizer of BRCA2 octamer, 102 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 7
The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells Nature Communications 11(1) (2020)
...Alam I, Livera G, Emmanuelle M, Wang P, Nandakumar J, Davies O, Shibuya H
RgGuinier 5.7 nm
Dmax 20.0 nm
VolumePorod 368 nm3

SASDTB9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 100 µs post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.3 nm
Dmax 15.3 nm
VolumePorod 730 nm3

SASDHC9 – Meiotic localizer of BRCA2 - MEILB1a2 monomer

Meiotic localizer of BRCA2 experimental SAS data
DAMMIF model
Sample: Meiotic localizer of BRCA2 monomer, 9 kDa Mus musculus protein
Buffer: 20 mM Tris pH 8.0, 150 mM KCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 7
The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells Nature Communications 11(1) (2020)
...Alam I, Livera G, Emmanuelle M, Wang P, Nandakumar J, Davies O, Shibuya H
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 43 nm3

SASDTC9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 500 µs post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.3 nm
Dmax 15.3 nm
VolumePorod 728 nm3

SASDTD9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1 ms (20 Hz) post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.3 nm
Dmax 15.3 nm
VolumePorod 742 nm3

SASDTE9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 1 ms (5 Hz) post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.1 nm
Dmax 15.4 nm
VolumePorod 706 nm3

SASDTF9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 10 ms post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.1 nm
Dmax 15.4 nm
VolumePorod 706 nm3

SASDTG9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate 100 ms post temperature jump (44 °C - 50 °C)

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 29
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.1 nm
Dmax 15.5 nm
VolumePorod 706 nm3

SASDTH9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 30 °C

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 4.1 nm
Dmax 15.3 nm
VolumePorod 768 nm3

SASDTJ9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 35 °C

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.3 nm
VolumePorod 765 nm3

SASDTK9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 40 °C

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.3 nm
VolumePorod 764 nm3

SASDTL9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate at 44 °C

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 28
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.2 nm
Dmax 15.2 nm
VolumePorod 728 nm3

SASDTM9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 5 µs post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.1 nm
VolumePorod 750 nm3

SASDTN9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 10 µs post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.1 nm
VolumePorod 750 nm3

SASDTP9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 50 µs post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.1 nm
VolumePorod 752 nm3

SASDTQ9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 100 µs post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.1 nm
VolumePorod 755 nm3

SASDTR9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 250 µs post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.3 nm
VolumePorod 741 nm3

SASDTS9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 500 µs post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.1 nm
VolumePorod 750 nm3

SASDTT9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 750 µs post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.3 nm
VolumePorod 739 nm3

SASDTU9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 1 ms post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.1 nm
VolumePorod 751 nm3

SASDTV9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 10 ms post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.5 nm
VolumePorod 733 nm3

SASDTW9 – HIV-1 Envelope Glycoprotein SOSIP from the CH848 isolate 100 ms post temperature jump (44 °C - 50 °C)

CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein experimental SAS data
CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH848.3.D0949.10.17chim.6R.DS.SOSIP.664 Env glycoprotein trimer, 211 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 14-ID-B (BioCARS), Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 30
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 15.3 nm
VolumePorod 732 nm3

SASDTX9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate including the 4.1 stabilization mutations at 25 °C

CH505TF.6R.SOSIP.664.v4.1 Env glycoprotein experimental SAS data
CH505TF.6R.SOSIP.664.v4.1 Env glycoprotein Kratky plot
Sample: CH505TF.6R.SOSIP.664.v4.1 Env glycoprotein trimer, 218 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.1 nm
Dmax 18.2 nm
VolumePorod 718 nm3

SASDTY9 – HIV-1 Envelope Glycoprotein SOSIP from a transmitted founder virus of the CH505 isolate at 25 °C

CH505TFchim.6R.SOSIP.664 Env glycoprotein experimental SAS data
CH505TFchim.6R.SOSIP.664 Env glycoprotein Kratky plot
Sample: CH505TFchim.6R.SOSIP.664 Env glycoprotein trimer, 217 kDa HIV-1 group M protein
Buffer: 15 mM HEPES, 150 mM NaCl, pH: 7.1
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 1
Microsecond dynamics control the HIV-1 Envelope conformation. Sci Adv 10(5):eadj0396 (2024)
...Alam SM, Haynes BF, Acharya P, Henderson R
RgGuinier 5.3 nm
Dmax 16.1 nm
VolumePorod 731 nm3

SASDDD8 – class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III

Probable exodeoxyribonuclease III protein XthA experimental SAS data
DAMMIF model
Sample: Probable exodeoxyribonuclease III protein XthA monomer, 32 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 12
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 2.4 nm
Dmax 7.3 nm
VolumePorod 56 nm3

SASDDN9 – Mycobacterium tuberculosis DNA Ligase A

DNA ligase A experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 5.2 nm
Dmax 16.7 nm
VolumePorod 870 nm3

SASDDV9 – Mycobacterium tuberculosis DNA LigaseA with Nicked DNA

DNA ligase ANicked DNA experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Nicked DNA dimer, 16 kDa DNA
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 4.4 nm
Dmax 14.8 nm
VolumePorod 262 nm3

SASDEW3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA)

DNA ligase AProbable exodeoxyribonuclease III protein XthA experimental SAS data
DAMMIF model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 6.2 nm
Dmax 23.9 nm
VolumePorod 662 nm3

SASDEX3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) in presence of nicked ds DNA

DNA ligase AProbable exodeoxyribonuclease III protein XthANicked DNA experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein
Nicked DNA monomer, 8 kDa DNA
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 13
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 4.5 nm
Dmax 17.1 nm
VolumePorod 117 nm3

SASDDQ8 – The complex formed between the class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III (XthA) bound to the BRCT domain from Mycobacterium tuberculosis DNA ligase

Probable exodeoxyribonuclease III protein XthAM. tb. LigA BRCT domain experimental SAS data
DAMFILT model
Sample: Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein
M. tb. LigA BRCT domain monomer, 15 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 9
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 3.7 nm
Dmax 18.5 nm
VolumePorod 112 nm3

SASDD88 – The BRCT domain from Mycobacterium tuberculosis DNA ligase

M.tb. LigA BRCT domain (DNA ligase A) experimental SAS data
DAMMIN model
Sample: M.tb. LigA BRCT domain (DNA ligase A) monomer, 13 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Jun 2
M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 1.6 nm
Dmax 3.7 nm
VolumePorod 23 nm3