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46 hits found for Chataigner

SASDL66 – High mannose neurofascin 155 immunoglobulin domains 1-6, 37.2 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 355 nm3

SASDL76 – High mannose neurofascin 155 immunoglobulin domains 1-6, 73.5 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 5.0 nm
Dmax 21.0 nm
VolumePorod 450 nm3

SASDL86 – High mannose neurofascin 155 immunoglobulin domains 1-6, 19.7 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.5 nm
Dmax 17.0 nm
VolumePorod 290 nm3

SASDL96 – High mannose neurofascin 155 immunoglobulin domains 1-6, 9.5 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.3 nm
Dmax 17.0 nm
VolumePorod 240 nm3

SASDLA6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 5.1 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.2 nm
Dmax 17.0 nm
VolumePorod 207 nm3

SASDLB6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 2.7 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.1 nm
Dmax 17.0 nm
VolumePorod 187 nm3

SASDLC6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 1.3 μM

Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 3.9 nm
Dmax 12.6 nm
VolumePorod 172 nm3

SASDLD6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 109.3 μM

Neurofascin T216A experimental SAS data
Neurofascin T216A Kratky plot
Sample: Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 25
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.3 nm
Dmax 18.0 nm
VolumePorod 212 nm3

SASDLE6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 21.9 μM

Neurofascin T216A experimental SAS data
Neurofascin T216A Kratky plot
Sample: Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 25
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 3.9 nm
Dmax 16.0 nm
VolumePorod 158 nm3

SASDLF6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 5.5 μM

Neurofascin T216A experimental SAS data
Neurofascin T216A Kratky plot
Sample: Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 25
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 150 nm3

SASDLG6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 1.1 μM

Neurofascin T216A experimental SAS data
Neurofascin T216A Kratky plot
Sample: Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jun 25
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 3.6 nm
Dmax 10.6 nm
VolumePorod 139 nm3

SASDLH6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 24.6 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 180 nm3

SASDLJ6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 12.3 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.9 nm
Dmax 23.0 nm
VolumePorod 166 nm3

SASDLK6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 6.2 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.9 nm
Dmax 23.0 nm
VolumePorod 155 nm3

SASDLL6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 3.1 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.9 nm
Dmax 15.8 nm
VolumePorod 118 nm3

SASDLM6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 21.8 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 5.2 nm
Dmax 23.0 nm
VolumePorod 154 nm3

SASDLN6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 10.9 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 150 nm3

SASDLP6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 5.5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 5.0 nm
Dmax 23.0 nm
VolumePorod 146 nm3

SASDLQ6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 2.7 μM

Contactin-1 I433V experimental SAS data
CUSTOM IN-HOUSE model
Sample: Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.9 nm
Dmax 14.5 nm
VolumePorod 100 nm3

SASDLR6 – Complex glycan contactin 1 ectodomain, 20 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 8.1 nm
Dmax 42.0 nm
VolumePorod 520 nm3

SASDLS6 – Complex glycan contactin 1 ectodomain, 10 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 8.0 nm
Dmax 43.0 nm
VolumePorod 530 nm3

SASDLT6 – Complex glycan contactin 1 ectodomain, 5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 8.0 nm
Dmax 43.0 nm
VolumePorod 530 nm3

SASDLU6 – Complex glycan contactin 1 ectodomain, 2.5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 8.0 nm
Dmax 25.7 nm
VolumePorod 350 nm3

SASDLV6 – High mannose glycan contactin 1 ectodomain, 20.5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 7.0 nm
Dmax 34.0 nm
VolumePorod 285 nm3

SASDLW6 – High mannose glycan contactin 1 ectodomain, 11.1 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 6.8 nm
Dmax 27.0 nm
VolumePorod 275 nm3

SASDLX6 – High mannose glycan contactin 1 ectodomain, 5.5 μM

Contactin-1 I433V experimental SAS data
Contactin-1 I433V Kratky plot
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 6.8 nm
Dmax 27.0 nm
VolumePorod 270 nm3

SASDLY6 – High mannose glycan contactin 1 ectodomain, 2.4 μM

Contactin-1 I433V experimental SAS data
CUSTOM IN-HOUSE model
Sample: Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 6.8 nm
Dmax 19.5 nm
VolumePorod 254 nm3

SASDNP8 – High mannose contactin-2 immunoglobulin domains1-6, 1μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.1 nm
Dmax 20.0 nm
VolumePorod 140 nm3

SASDNQ8 – High mannose contactin 2 immunoglobulin domains1-6, 3μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.3 nm
Dmax 20.0 nm
VolumePorod 300 nm3

SASDNR8 – High mannose contactin-2 immunoglobulin domains1-6, 6μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.4 nm
Dmax 21.0 nm
VolumePorod 325 nm3

SASDNS8 – High mannose contactin-2 immunoglobulin domains1-6, 13μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.5 nm
Dmax 21.4 nm
VolumePorod 350 nm3

SASDNT8 – High mannose contactin-2 immunoglobulin domains1-6, 26μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 6.0 nm
Dmax 30.0 nm
VolumePorod 380 nm3

SASDNU8 – High mannose contactin-2 immunoglobulin domains1-6, 52μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 6.1 nm
Dmax 32.0 nm
VolumePorod 408 nm3

SASDNV8 – Complex glycan contactin-2 immunoglobulin domains1-6, 1μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.6 nm
Dmax 25.0 nm
VolumePorod 230 nm3

SASDNW8 – Complex glycan contactin-2 immunoglobulin domains1-6, 3μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.4 nm
Dmax 25.0 nm
VolumePorod 185 nm3

SASDNX8 – Complex glycan contactin-2 immunoglobulin domains1-6, 5μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.5 nm
Dmax 22.0 nm
VolumePorod 353 nm3

SASDNY8 – Complex glycan contactin-2 immunoglobulin domains1-6, 11μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.5 nm
Dmax 22.0 nm
VolumePorod 365 nm3

SASDNZ8 – Complex glycan contactin-2 immunoglobulin domains1-6, 24μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.6 nm
Dmax 22.0 nm
VolumePorod 390 nm3

SASDN29 – Complex glycan contactin-2 immunoglobulin domains1-6, 51μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 7
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 5.8 nm
Dmax 32.0 nm
VolumePorod 412 nm3

SASDN39 – Complex glycan contactin-2 ectodomain, 1 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 9.3 nm
Dmax 45.0 nm
VolumePorod 780 nm3

SASDN49 – Complex glycan contactin-2 ectodomain, 2 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 10.3 nm
Dmax 60.0 nm
VolumePorod 1290 nm3

SASDN59 – Complex glycan contactin-2 ectodomain, 3 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 11.2 nm
Dmax 60.0 nm
VolumePorod 1540 nm3

SASDN69 – Complex glycan contactin-2 ectodomain, 6 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 11.0 nm
Dmax 70.0 nm
VolumePorod 1900 nm3

SASDN79 – Complex glycan contactin-2 ectodomain, 12 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 11.3 nm
Dmax 66.0 nm
VolumePorod 1820 nm3

SASDN89 – Complex glycan contactin-2 ectodomain, 23 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 12.5 nm
Dmax 65.0 nm
VolumePorod 2044 nm3

SASDN99 – Complex glycan contactin-2 ectodomain, 50 μM

Contactin-2 experimental SAS data
Contactin-2 Kratky plot
Sample: Contactin-2 trimer, 389 kDa Mus musculus protein
Buffer: 25 mM HEPES, 500 mM NaCl, 500 mM imidazole, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 17
Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B
RgGuinier 14.2 nm
Dmax 65.0 nm
VolumePorod 2190 nm3