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38 hits found for Deane

SASDKF3 – Human telomere monomer G4 hybrid-2 form (2JSL)

Human telomere G-quadruplex hybrid-2 form experimental SAS data
DAMMIN model
Sample: Human telomere G-quadruplex hybrid-2 form monomer, 8 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Aug 14
The solution structures of higher-order human telomere G-quadruplex multimers. Nucleic Acids Res (2021)
Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.8 nm
VolumePorod 9 nm3

SASDPF3 – Receptor-type tyrosine-protein phosphatase kappa extracellular domains (PTPRK-ECDs)

Receptor-type tyrosine-protein phosphatase kappa experimental SAS data
DAMMIN model
Sample: Receptor-type tyrosine-protein phosphatase kappa monomer, 82 kDa Homo sapiens protein
Buffer: 50 mM MES, 250 mM NaCl, 3% v/v glycerol,, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 13
Determinants of receptor tyrosine phosphatase homophilic adhesion: structural comparison of PTPRK and PTPRM extracellular domains Journal of Biological Chemistry :102750 (2022)
...Deane J
RgGuinier 7.0 nm
Dmax 26.0 nm
VolumePorod 252 nm3

SASDKG3 – Human wild-type telomere 48-mer d(TTAGGG)8

Human Telomere Repeat (TTAGGG)8 experimental SAS data
DAMMIN model
Sample: Human Telomere Repeat (TTAGGG)8 monomer, 15 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Aug 14
The solution structures of higher-order human telomere G-quadruplex multimers. Nucleic Acids Res (2021)
Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO
RgGuinier 1.9 nm
Dmax 6.4 nm
VolumePorod 16 nm3

SASDPG3 – Receptor-type tyrosine-protein phosphatase mu extracellular domains (PTPRM-ECDs)

Receptor-type tyrosine-protein phosphatase mu experimental SAS data
DAMMIN model
Sample: Receptor-type tyrosine-protein phosphatase mu monomer, 82 kDa Homo sapiens protein
Buffer: 50 mM MES, 250 mM NaCl, 3% v/v glycerol,, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 13
Determinants of receptor tyrosine phosphatase homophilic adhesion: structural comparison of PTPRK and PTPRM extracellular domains Journal of Biological Chemistry :102750 (2022)
...Deane J
RgGuinier 7.2 nm
Dmax 26.0 nm
VolumePorod 255 nm3

SASDKH3 – Human wild-type telomere 72mer d(TTAGGG)12

Human Telomere Repeat (TTAGGG)12 experimental SAS data
DAMMIF model
Sample: Human Telomere Repeat (TTAGGG)12 monomer, 23 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Aug 14
The solution structures of higher-order human telomere G-quadruplex multimers. Nucleic Acids Res (2021)
Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 26 nm3

SASDKJ3 – Human telomere DNA d(TTAGGG)96

Human Telomere 96mer experimental SAS data
DAMMIF model
Sample: Human Telomere 96mer monomer, 31 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19
The solution structures of higher-order human telomere G-quadruplex multimers. Nucleic Acids Res (2021)
Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO
RgGuinier 3.2 nm
Dmax 10.9 nm
VolumePorod 38 nm3

SASDB95 – Shigella outer membrane protein IcsA autotransporter

Outer membrane protein IcsA (53-758) experimental SAS data
DAMMIN model
Sample: Outer membrane protein IcsA (53-758) monomer, 72 kDa Shigella flexneri protein
Buffer: 50 mM Tris 150 mM NaCl 10 mM CaCl2 3% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 May 18
The Shigella Virulence Factor IcsA Relieves N-WASP Autoinhibition by Displacing the Verprolin Homology/Cofilin/Acidic (VCA) Domain. J Biol Chem 292(1):134-145 (2017)
...Deane JE
RgGuinier 3.7 nm
Dmax 13.2 nm
VolumePorod 103 nm3

SASDJA6 – Complement C5

Complement C5 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Complement C5 monomer, 186 kDa Homo sapiens protein
Buffer: 20mM Tris pH, 75mM NaCl, and 3% glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 12
The allosteric modulation of Complement C5 by knob domain peptides. Elife 10 (2021)
...Deane CM, Svergun D, Lawson AD, van den Elsen JM
RgGuinier 4.8 nm
Dmax 17.6 nm
VolumePorod 392 nm3

SASDNB7 – C-terminal domain of pUL21 with N-terminal His-tag

Tegument protein UL21 (C-terminal domain) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
...Deane J, Jeffries C, Crump C, Graham S
RgGuinier 2.2 nm
Dmax 8.5 nm
VolumePorod 49 nm3

SASDNC7 – miniCERTL with N-terminal His-tag

Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
...Deane J, Jeffries C, Crump C, Graham S
RgGuinier 2.7 nm
Dmax 9.1 nm
VolumePorod 71 nm3

SASDND7 – Complex of H6-pUL21C and H6-miniCERTL

Tegument protein UL21 (C-terminal domain)Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
...Deane J, Jeffries C, Crump C, Graham S
RgGuinier 3.3 nm
Dmax 13.6 nm
VolumePorod 84 nm3

SASDNE7 – Complex of H6-miniCERTL and full-length pUL21-H6

Tegument protein UL21Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) experimental SAS data
GASBOR model
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT Journal of Biological Chemistry :102589 (2022)
...Deane J, Jeffries C, Crump C, Graham S
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 158 nm3

SASDKW8 – Full-length pUL21 with C-terminal His-tag

Tegument protein UL21 experimental SAS data
Full-length pUL21 with C-terminal His-tag Rg histogram
Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein
Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25
pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread. PLoS Pathog 17(8):e1009824 (2021)
...Deane JE, Firth AE, Jeffries CM, Crump CM, Graham SC
RgGuinier 4.2 nm
Dmax 18.1 nm
VolumePorod 90 nm3

SASDHM3 – Human Telomerase Reverse Transcriptase Core Promoter G-rich Region (hTERT_WT) DNA

Human Telomerase Reverse Transcriptase Core Promoter G-rich Region experimental SAS data
DAMMIF model
Sample: Human Telomerase Reverse Transcriptase Core Promoter G-rich Region monomer, 22 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Oct 5
The hTERT core promoter forms three parallel G-quadruplexes. Nucleic Acids Res (2020)
Monsen RC, DeLeeuw L, Dean WL, Gray RD, Sabo TM, Chakravarthy S, Chaires JB, Trent JO
RgGuinier 2.2 nm
Dmax 8.1 nm
VolumePorod 28 nm3

SASDMJ6 – cMyc-8

cMyc promoter 8-tract G-quadruplex experimental SAS data
CHIMERA model
Sample: cMyc promoter 8-tract G-quadruplex monomer, 11 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.4 nm
Dmax 4.6 nm
VolumePorod 13 nm3

SASDHN3 – Antiparallel Hairpin (AH) DNA

Antiparallel Hairpin experimental SAS data
DAMMIF model
Sample: Antiparallel Hairpin monomer, 21 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 3
The hTERT core promoter forms three parallel G-quadruplexes. Nucleic Acids Res (2020)
Monsen RC, DeLeeuw L, Dean WL, Gray RD, Sabo TM, Chakravarthy S, Chaires JB, Trent JO
RgGuinier 2.5 nm
Dmax 8.1 nm
VolumePorod 28 nm3

SASDMM6 – cMyc-12

cMyc promoter 12-tract G-quadruplex experimental SAS data
cMyc promoter 12-tract G-quadruplex Kratky plot
Sample: cMyc promoter 12-tract G-quadruplex monomer, 23 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 22
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 2.3 nm
Dmax 8.4 nm
VolumePorod 29 nm3

SASDHP3 – Optimized Parallel DNA

Optimized Parallel experimental SAS data
DAMFILT model
Sample: Optimized Parallel monomer, 18 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 3
The hTERT core promoter forms three parallel G-quadruplexes. Nucleic Acids Res (2020)
Monsen RC, DeLeeuw L, Dean WL, Gray RD, Sabo TM, Chakravarthy S, Chaires JB, Trent JO
RgGuinier 1.7 nm
Dmax 5.0 nm
VolumePorod 17 nm3

SASDM36 – c-Myc12 DnaseI Treated G-quadruplex

c-Myc 12-tract G-quadruplex monomer Dnase I treated experimental SAS data
CHIMERA model
Sample: c-Myc 12-tract G-quadruplex monomer Dnase I treated monomer, 22 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Sep 29
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 25 nm3

SASDHQ3 – Human Telomerase Reverse Transcriptase Core Promoter (hTERT) PQS2-PQS3 region, WT_PQS23

hTERT core promoter PQS2-PQS3 region experimental SAS data
DAMFILT model
Sample: hTERT core promoter PQS2-PQS3 region monomer, 14 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 3
The hTERT core promoter forms three parallel G-quadruplexes. Nucleic Acids Res (2020)
Monsen RC, DeLeeuw L, Dean WL, Gray RD, Sabo TM, Chakravarthy S, Chaires JB, Trent JO
RgGuinier 1.7 nm
Dmax 5.4 nm
VolumePorod 18 nm3

SASDMK6 – cKit-8

cKit promoter 8-tract G-quadruplex experimental SAS data
CHIMERA model
Sample: cKit promoter 8-tract G-quadruplex monomer, 12 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.5 nm
Dmax 4.7 nm
VolumePorod 15 nm3

SASDHR3 – Antiparallel Hairpin PQS2-PQS3 segment DNA, AH_PQS23

Antiparallel Hairpin PQS2-PQS3 segment experimental SAS data
DAMFILT model
Sample: Antiparallel Hairpin PQS2-PQS3 segment monomer, 14 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 3
The hTERT core promoter forms three parallel G-quadruplexes. Nucleic Acids Res (2020)
Monsen RC, DeLeeuw L, Dean WL, Gray RD, Sabo TM, Chakravarthy S, Chaires JB, Trent JO
RgGuinier 2.0 nm
Dmax 6.4 nm
VolumePorod 18 nm3

SASDMN6 – cKit-12

cKit promoter 12-tract G-quadruplex experimental SAS data
CHIMERA model
Sample: cKit promoter 12-tract G-quadruplex monomer, 20 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 22
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 26 nm3

SASDM46 – c-Kit12 DNase I Digest

c-Kit 12-tract G-quadruplex monomer Dnase I treated experimental SAS data
c-Kit 12-tract G-quadruplex monomer Dnase I treated Kratky plot
Sample: c-Kit 12-tract G-quadruplex monomer Dnase I treated monomer, 20 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Sep 29
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.6 nm
Dmax 5.7 nm
VolumePorod 16 nm3

SASDML6 – kRas-8

kRas promoter 8-tract G-quadruplex experimental SAS data
CHIMERA model
Sample: kRas promoter 8-tract G-quadruplex monomer, 14 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 16 nm3

SASDM56 – 2KZD

TERT promoter G-quadruplex antiparallel experimental SAS data
CHIMERA model
Sample: TERT promoter G-quadruplex antiparallel monomer, 6 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 13
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.6 nm
VolumePorod 7 nm3

SASDM76 – 201D

DNA oligonucleotide G4(T4G4)3 experimental SAS data
CHIMERA model
Sample: DNA oligonucleotide G4(T4G4)3 monomer, 9 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 12
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 4.1 nm
VolumePorod 10 nm3

SASDM86 – 6GH0

cKIT 2-tetrad promoter GQ antiparallel experimental SAS data
CHIMERA model
Sample: cKIT 2-tetrad promoter GQ antiparallel monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 21
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.0 nm
Dmax 3.1 nm
VolumePorod 8 nm3

SASDM96 – 2GKU

human telomere 24mer hybrid-1 experimental SAS data
CHIMERA model
Sample: human telomere 24mer hybrid-1 monomer, 8 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.8 nm
VolumePorod 8 nm3

SASDMA6 – 5I2V

kRas promoter GQ hybrid experimental SAS data
CHIMERA model
Sample: kRas promoter GQ hybrid monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 4.9 nm
VolumePorod 10 nm3

SASDMB6 – 6L92

WNT promoter GQ hybrid form experimental SAS data
CHIMERA model
Sample: WNT promoter GQ hybrid form monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 21
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.9 nm
VolumePorod 8 nm3

SASDMC6 – 2KQG

cKit promoter GQ parallel experimental SAS data
CHIMERA model
Sample: cKit promoter GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.6 nm
Dmax 3.2 nm
VolumePorod 14 nm3

SASDMD6 – 2LBY

cMyc promoter GQ parallel experimental SAS data
CHIMERA model
Sample: cMyc promoter GQ parallel monomer, 6 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.9 nm
VolumePorod 7 nm3

SASDME6 – 6NEB

cMyc promoter GQ 1:6:1 loop parallel experimental SAS data
CHIMERA model
Sample: cMyc promoter GQ 1:6:1 loop parallel monomer, 9 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.4 nm
Dmax 4.5 nm
VolumePorod 10 nm3

SASDMF6 – 1XAV

cMyc promoter modified GQ parallel experimental SAS data
CHIMERA model
Sample: cMyc promoter modified GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 4.1 nm
VolumePorod 9 nm3

SASDM66 – 2KZE

TERT promoter G-quadruplex parallel experimental SAS data
CHIMERA model
Sample: TERT promoter G-quadruplex parallel monomer, 6 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Aug 11
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 4.2 nm
VolumePorod 8 nm3

SASDMG6 – 5CMX

two-tetrad antiparallel GQ with duplex junction experimental SAS data
CHIMERA model
Sample: two-tetrad antiparallel GQ with duplex junction monomer, 10 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 3
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.5 nm
Dmax 5.2 nm
VolumePorod 12 nm3

SASDMH6 – 2M27

VEGF promoter GQ parallel experimental SAS data
CHIMERA model
Sample: VEGF promoter GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
Monsen RC, DeLeeuw LW, Dean WL, Gray RD, Chakravarthy S, Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 3.8 nm
VolumePorod 8 nm3