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11 hits found for Degenhardt

SASDL26 – CBD12 of CALX1.1 (apo state)

AT07459p experimental SAS data
CBD12 of CALX1.1 (apo state) Rg histogram
Sample: AT07459p monomer, 30 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, 5 mM β-mercaptoethanol, 200 mM NaCl, 1% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8
Molecular insights on CALX-CBD12 inter-domain dynamics from MD simulations, RDCs and SAXS. Biophys J (2021)
...Degenhardt MF, Vitale PAM, Abiko LA, Zacharias M, Sattler M, Oliveira CLP, Salinas RK
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 41 nm3

SASDL36 – CBD12 of CALX1.1 in the calcium-bound state

AT07459p experimental SAS data
CBD12 of CALX1.1 in the calcium-bound state Rg histogram
Sample: AT07459p monomer, 30 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, 5 mM β-mercaptoethanol, 200 mM NaCl, 1% v/v glycerol, 0.8 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Apr 13
Molecular insights on CALX-CBD12 inter-domain dynamics from MD simulations, RDCs and SAXS. Biophys J (2021)
...Degenhardt MF, Vitale PAM, Abiko LA, Zacharias M, Sattler M, Oliveira CLP, Salinas RK
RgGuinier 2.7 nm
Dmax 9.3 nm
VolumePorod 40 nm3

SASDTA7 – RNase P RNA in the presence of 0.1 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 0.1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 6.1 nm
Dmax 21.8 nm
VolumePorod 813 nm3

SASDTB7 – RNase P RNA in the presence of 1.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 5.2 nm
Dmax 15.6 nm
VolumePorod 370 nm3

SASDTC7 – RNase P RNA in the presence of 1.5 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl,1.5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 5.0 nm
Dmax 16.2 nm
VolumePorod 352 nm3

SASDTD7 – RNase P RNA in the presence of 2.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 2.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 5.0 nm
Dmax 15.7 nm
VolumePorod 316 nm3

SASDTE7 – RNase P RNA in the presence of 2.5 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 2.5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.9 nm
Dmax 15.5 nm
VolumePorod 306 nm3

SASDTF7 – RNase P RNA in the presence of 3.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 3 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.9 nm
Dmax 15.5 nm
VolumePorod 301 nm3

SASDTG7 – RNase P RNA in the presence of 5.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.7 nm
Dmax 14.6 nm
VolumePorod 277 nm3

SASDTH7 – RNase P RNA in the presence of 8.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl, 8.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.7 nm
Dmax 14.8 nm
VolumePorod 280 nm3

SASDTJ7 – RNase P RNA in the presence of 10.0 mM MgCl2

RNase P RNA experimental SAS data
RNase P RNA Kratky plot
Sample: RNase P RNA monomer, 135 kDa Geobacillus stearothermophilus RNA
Buffer: 100 mM NaCl,10.0 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BIOSAXS-2000 [duplicate], Center for Cancer Research,National Cancer Institute on 2023 Nov 3
The conformational space of RNase P RNA in solution Nature (2024)
...Degenhardt M, Skeparnias I, Degenhardt H, Bhandari Y, Yu P, Stagno J, Fan L, Zhang J, Wang Y
RgGuinier 4.8 nm
Dmax 14.6 nm
VolumePorod 285 nm3