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17 hits found for Forneris

SASDFU3 – Lysine-specific Demethylase (LSD2)

Lysyne-specific Demethylase LSD2 experimental SAS data
Lysyne-specific Demethylase LSD2 Kratky plot
Sample: Lysyne-specific Demethylase LSD2 monomer, 89 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 11
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
...Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 124 nm3

SASDFV3 – Cytokine-like nuclear factor dehydrogenase domain, NPAC DH (NPAC delta-261)

NPAC dehydrogenase domain experimental SAS data
NPAC dehydrogenase domain Kratky plot
Sample: NPAC dehydrogenase domain tetramer, 125 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 26
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
...Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
RgGuinier 3.5 nm
Dmax 11.0 nm
VolumePorod 167 nm3

SASDFW3 – Cytokine-like nuclear factor dehydrogenase domain, NPAC DH, plus native linker (NPAC delta-205)

NPAC linker+DH (delta-205) experimental SAS data
NPAC linker+DH (delta-205) Kratky plot
Sample: NPAC linker+DH (delta-205) tetramer, 150 kDa Homo sapiens protein
Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 26
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
...Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
RgGuinier 4.0 nm
Dmax 11.4 nm
VolumePorod 240 nm3

SASDFX3 – Semi-synthetic nucleosome core particle (NCP)

Histone H3Histone H4Histone H2aHistone H2b147bp 601 Widom sequence experimental SAS data
Histone H3 Histone H4 Histone H2a Histone H2b 147bp 601 Widom sequence Kratky plot
Sample: Histone H3 dimer, 31 kDa Xenopus laevis protein
Histone H4 dimer, 22 kDa Xenopus laevis protein
Histone H2a dimer, 28 kDa Xenopus laevis protein
Histone H2b dimer, 27 kDa Xenopus laevis protein
147bp 601 Widom sequence monomer, 45 kDa synthetic construct DNA
Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 11
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
...Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
RgGuinier 4.4 nm
Dmax 12.8 nm
VolumePorod 353 nm3

SASDFY3 – Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205)

Lysyne-specific Demethylase LSD2NPAC linker+DH (delta-205)Histone H3Histone H4Histone H2aHistone H2b147bp 601 Widom sequence experimental SAS data
Lysyne-specific Demethylase LSD2 NPAC linker+DH (delta-205) Histone H3 Histone H4 Histone H2a Histone H2b 147bp 601 Widom sequence Kratky plot
Sample: Lysyne-specific Demethylase LSD2 monomer, 89 kDa Homo sapiens protein
NPAC linker+DH (delta-205) tetramer, 150 kDa Homo sapiens protein
Histone H3 dimer, 31 kDa Xenopus laevis protein
Histone H4 dimer, 22 kDa Xenopus laevis protein
Histone H2a dimer, 28 kDa Xenopus laevis protein
Histone H2b dimer, 27 kDa Xenopus laevis protein
147bp 601 Widom sequence monomer, 45 kDa synthetic construct DNA
Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3
Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 11
A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex. Cell Rep 27(2):387-399.e7 (2019)
...Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
RgGuinier 7.8 nm
Dmax 30.4 nm
VolumePorod 1100 nm3

SASDDW4 – Procollagen lysyl hydroxylase LH3 measured using SEC-SAXS

Procollagen lysyl hydroxylase LH3 experimental SAS data
GASBOR model
Sample: Procollagen lysyl hydroxylase LH3 dimer, 180 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 28
Molecular architecture of the multifunctional collagen lysyl hydroxylase and glycosyltransferase LH3. Nat Commun 9(1):3163 (2018)
...Forneris F
RgGuinier 5.1 nm
Dmax 21.0 nm
VolumePorod 268 nm3

SASDES5 – Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 (mmNT-SRCR3) - from SEC-SAXS

Mouse Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Mouse Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 monomer, 13 kDa Mus musculus protein
Buffer: 25 mM HEPES, 0.1 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 20
Structural characterization of the third scavenger receptor cysteine-rich domain of murine neurotrypsin. Protein Sci 28(4):746-755 (2019)
...Forneris F
RgGuinier 1.5 nm
Dmax 4.5 nm
VolumePorod 24 nm3

SASDJX7 – Labrum-Interacting Proteins from Saliva 2 (34k-2) - SEC-SAXS

Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2 experimental SAS data
ALLOSMOD model
Sample: Similar to Aedes aegypti 34 kDa salivary secreted protein 34k-2 monomer, 35 kDa Aedes albopictus protein
Buffer: 25 mM HEPES, 0.1 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Sep 3
A salivary factor binds a cuticular protein and modulates biting by inducing morphological changes in the mosquito labrum. Curr Biol (2022)
...Forneris F, Gabrieli P
RgGuinier 2.7 nm
Dmax 8.4 nm
VolumePorod 50 nm3

SASDJN8 – L19-IL2 dimeric immunocytokine

L19-IL2 dimeric immunocytokine experimental SAS data
ALLOSMOD model
Sample: L19-IL2 dimeric immunocytokine dimer, 84 kDa synthetic construct protein
Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 23
Inference of molecular structure for characterization and improvement of clinical grade immunocytokines Journal of Structural Biology :107696 (2021)
...Forneris F
RgGuinier 4.1 nm
Dmax 11.9 nm
VolumePorod 128 nm3

SASDJP8 – L19L19-IL2 immunocytokine monomer

L19L19-IL2 immunocytokine experimental SAS data
GASBOR model
Sample: L19L19-IL2 immunocytokine monomer, 69 kDa synthetic construct protein
Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 4
Inference of molecular structure for characterization and improvement of clinical grade immunocytokines Journal of Structural Biology :107696 (2021)
...Forneris F
RgGuinier 3.6 nm
Dmax 10.8 nm
VolumePorod 93 nm3

SASDJQ8 – IL12-L19L19 immunocytokine monomer

IL12-L19L19 immunocytokine experimental SAS data
GASBOR model
Sample: IL12-L19L19 immunocytokine monomer, 112 kDa synthetic construct protein
Buffer: 25 mM HEPES/NaOH, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 4
Inference of molecular structure for characterization and improvement of clinical grade immunocytokines Journal of Structural Biology :107696 (2021)
...Forneris F
RgGuinier 4.5 nm
Dmax 14.2 nm
VolumePorod 224 nm3

SASDLM9 – Minimal hepatocyte growth factor mimic K1K1

minimal hepatocyte growth factor mimic K1K1 experimental SAS data
GASBOR model
Sample: minimal hepatocyte growth factor mimic K1K1 monomer, 19 kDa synthetic construct protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 3
Dimerization of kringle 1 domain from hepatocyte growth factor/scatter factor provides a potent MET receptor agonist Life Science Alliance 5(12):e202201424 (2022)
...Forneris F, Adriaenssens E, Drobecq H, Iamele L, Dubuquoy L, Melnyk O, Gherardi E, de Jonge H, Vicogne J
RgGuinier 2.2 nm
Dmax 6.6 nm
VolumePorod 22 nm3

SASDLN9 – Hepatocyte growth factor HGF/SF receptor - fragment MET567

Hepatocyte growth factor receptor experimental SAS data
GASBOR model
Sample: Hepatocyte growth factor receptor monomer, 62 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 27
Dimerization of kringle 1 domain from hepatocyte growth factor/scatter factor provides a potent MET receptor agonist Life Science Alliance 5(12):e202201424 (2022)
...Forneris F, Adriaenssens E, Drobecq H, Iamele L, Dubuquoy L, Melnyk O, Gherardi E, de Jonge H, Vicogne J
RgGuinier 3.2 nm
Dmax 11.7 nm
VolumePorod 126 nm3

SASDLP9 – Hepatocyte growth factor HGF/SF receptor - fragment MET567 in complex with the minimal hepatocyte growth factor mimic K1K1

minimal hepatocyte growth factor mimic K1K1Hepatocyte growth factor receptor experimental SAS data
CORAL model
Sample: minimal hepatocyte growth factor mimic K1K1 monomer, 19 kDa synthetic construct protein
Hepatocyte growth factor receptor monomer, 62 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 23
Dimerization of kringle 1 domain from hepatocyte growth factor/scatter factor provides a potent MET receptor agonist Life Science Alliance 5(12):e202201424 (2022)
...Forneris F, Adriaenssens E, Drobecq H, Iamele L, Dubuquoy L, Melnyk O, Gherardi E, de Jonge H, Vicogne J
RgGuinier 3.7 nm
Dmax 14.3 nm
VolumePorod 136 nm3

SASDN78 – Pikachurin N-terminal FnIII(1-2) fragment (SEC-SAXS)

Pikachurin N-terminal FnIII(1-2) domains experimental SAS data
GASBOR model
Sample: Pikachurin N-terminal FnIII(1-2) domains monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179 Science Signaling 16(795) (2023)
...Forneris F, Martemyanov K
RgGuinier 3.4 nm
Dmax 16.4 nm
VolumePorod 44 nm3

SASDN68 – Pikachurin C-terminal LG3 domain (SEC-SAXS)

Pikachurin experimental SAS data
GASBOR model
Sample: Pikachurin monomer, 22 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179 Science Signaling 16(795) (2023)
...Forneris F, Martemyanov K
RgGuinier 2.2 nm
Dmax 8.2 nm
VolumePorod 48 nm3

SASDN88 – Pikachurin C-terminal EGF-LG3 fragment (SEC-SAXS)

Pikachurin experimental SAS data
GASBOR model
Sample: Pikachurin monomer, 26 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Mar 3
Structure of the photoreceptor synaptic assembly of the extracellular matrix protein pikachurin with the orphan receptor GPR179 Science Signaling 16(795) (2023)
...Forneris F, Martemyanov K
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 52 nm3