Small Angle Scattering Biological Data Bank SASBDB


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12 hits found for Goretzki

SASDSY6 – Outer membrane protein MIP (LpMIP) apo state

Outer membrane protein MIP experimental SAS data

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

Legionella pneumophila macrophage infectivity potentiator protein appendage domains modulate protein dynamics and inhibitor binding International Journal of Biological Macromolecules :126366 (2023)
...Goretzki B, Dajka M, Tebbe F, Harder J, Krajczy P, Joseph B, Hausch F, Guskov A, Hellmich U

R_g^Guinier	3.1	nm
D_max	10.0	nm
Volume^Porod	66	nm³

SASDSZ6 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK095

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

R_g^Guinier	3.1	nm
D_max	10.2	nm
Volume^Porod	65	nm³

SASDS27 – Outer membrane protein MIP (LpMIP) with bound inhibitor JK236

Sample:	Outer membrane protein MIP dimer, 46 kDa Legionella pneumophila subsp. … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 10 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Mar 16

R_g^Guinier	3.1	nm
D_max	9.4	nm
Volume^Porod	63	nm³

SASDQE8 – G. gallus TRPV4 Ankyrin Repeat Domain

Transient receptor potential cation channel subfamily V member 4 experimental SAS data

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 28 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

Crosstalk between regulatory elements in disordered TRPV4 N-terminus modulates lipid-dependent channel activity Nature Communications 14(1) (2023)
Goretzki B, Wiedemann C, McCray B, Schäfer S, Jansen J, Tebbe F, Mitrovic S, Nöth J, Cabezudo A, Donohue J, Jeffries C, Steinchen W, Stengel F, Sumner C, Hummer G, Hellmich U

R_g^Guinier	2.3	nm
D_max	11.5	nm
Volume^Porod	41	nm³

SASDQF8 – G. gallus TRPV4 N-terminal Domain

G. gallus TRPV4 N-terminal Domain Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 43 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	3.7	nm
D_max	19.0	nm
Volume^Porod	59	nm³

SASDQG8 – G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111

G. gallus TRPV4 N-terminal Domain, mutant 107-KRWRR-111 to 107-AAWAA-111 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 42 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	4.0	nm
D_max	19.5	nm
Volume^Porod	78	nm³

SASDQH8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54

G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N54 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 37 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.6	nm
D_max	9.5	nm
Volume^Porod	64	nm³

SASDQJ8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97

G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N97 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 33 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.5	nm
D_max	11.9	nm
Volume^Porod	41	nm³

SASDQK8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N104

Transient receptor potential cation channel subfamily V member 4 Kratky plot

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 32 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.8	nm
D_max	13.0	nm
Volume^Porod	49	nm³

SASDQL8 – G. gallus TRPV4 N-terminal Domain, deletion mutant ∆N120

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 29 kDa Gallus gallus protein
Buffer:	20 mM Tris, 300 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Mar 16

R_g^Guinier	2.7	nm
D_max	12.5	nm
Volume^Porod	43	nm³

SASDQM8 – G. gallus TRPV4 intrinsically disordered N-terminus

G. gallus TRPV4 intrinsically disordered N-terminus Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 15 kDa Gallus gallus protein
Buffer:	20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	3.2	nm
D_max	14.5	nm
Volume^Porod	59	nm³

SASDQN8 – G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111

G. gallus TRPV4 intrinsically disordered N-terminus, mutation 107-KRWRR-111 to 107-AAWAA-111 Rg histogram

Sample:	Transient receptor potential cation channel subfamily V member 4 monomer, 14 kDa Gallus gallus protein
Buffer:	20 mM Tris, 100 mM NaCl, 10 mM DTT, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 16

R_g^Guinier	3.3	nm
D_max	14.5	nm
Volume^Porod	37	nm³

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