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6 hits found for Gross

SASDNJ5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1)

3-phosphoinositide-dependent protein kinase 1 experimental SAS data
DAMMIF model
Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
...Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia ...
RgGuinier 3.5 nm
Dmax 11.2 nm
VolumePorod 107 nm3

SASDNK5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1) in the presence of HYG8 (2-O-benzoyl-Ins(1,3,4,5,6)P5)

3-phosphoinositide-dependent protein kinase 12-O-benzoyl-Ins(1,3,4,5,6)P5 experimental SAS data
DAMMIF model
Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein
2-O-benzoyl-Ins(1,3,4,5,6)P5 monomer, 1 kDa synthetic construct
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, 1 μM HYG8, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
...Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia ...
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 98 nm3

SASDNL5 – Double mutant catalytic domain of 3-phosphoinositide-dependent protein kinase 1 (PDK1 50–359; Y188G Q292A)

3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) experimental SAS data
SREFLEX model
Sample: 3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
...Gross L, Riley A, Zehnder K, Ghode A, Klinke S, Anand G, Paris K, Winkel A, Herbrand A, Godage H, Cozier G, Süß E, Schulze J, Pastor-Flores D, Bollini M, Cappellari M, Svergun D, Gräwert M, Aramendia ...
RgGuinier 2.4 nm
Dmax 7.0 nm
VolumePorod 57 nm3

SASDU88 – SEC-SAXS of the fusion protein MPB-MabR(Rv2242) - monomeric peak

Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)Uncharacterized protein Rv2242 experimental SAS data
ALPHAFOLD model
Sample: Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) monomer, 40 kDa Escherichia coli (strain … protein
Uncharacterized protein Rv2242 monomer, 43 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH:
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3
Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family Heliyon :e40494 (2024)
...Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R
RgGuinier 2.8 nm
Dmax 8.5 nm
VolumePorod 113 nm3

SASDU98 – SEC-SAXS of the fusion protein MPB-MabR(Rv2242) - dimeric peak

Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)Uncharacterized protein Rv2242 experimental SAS data
ALPHAFOLD model
Sample: Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) dimer, 80 kDa Escherichia coli (strain … protein
Uncharacterized protein Rv2242 dimer, 86 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH:
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3
Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family Heliyon :e40494 (2024)
...Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R
RgGuinier 3.7 nm
Dmax 10.8 nm
VolumePorod 345 nm3

SASDUA8 – SEC-SAXS of the fusion protein MPB-MabR(Rv2242) - tetrameric peak

Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)Uncharacterized protein Rv2242 experimental SAS data
ALPHAFOLD model
Sample: Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A) tetramer, 161 kDa Escherichia coli (strain … protein
Uncharacterized protein Rv2242 tetramer, 171 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH:
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3
Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family Heliyon :e40494 (2024)
...Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R
RgGuinier 5.2 nm
Dmax 15.8 nm
VolumePorod 826 nm3