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13 hits found for Johansen

SASDM42 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
Johansen NT, Bonaccorsi M, Bengtsen T, Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Li...
RgGuinier 4.2 nm
Dmax 13.9 nm

SASDM52 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles with bound Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
Johansen NT, Bonaccorsi M, Bengtsen T, Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Li...
RgGuinier 4.3 nm
Dmax 13.9 nm

SASDM62 – Cobalt/magnesium transport protein CorA in matched-out deuterated nanodiscs without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
Johansen NT, Bonaccorsi M, Bengtsen T, Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Li...
RgGuinier 4.8 nm
Dmax 14.2 nm

SASDM72 – Cobalt/magnesium transport protein CorA in match-out deuterated nanodiscs with bound Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
Johansen NT, Bonaccorsi M, Bengtsen T, Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Li...
RgGuinier 4.9 nm
Dmax 14.3 nm

SASDL33 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with paused-flow SEC-SANS

Proton-gated ion channel experimental SAS data
GROMACS model
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 22
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
...Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 274 nm3

SASDL43 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 3.0 measured with paused-flow SEC-SANS

Proton-gated ion channel experimental SAS data
GROMACS model
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM citrate, 150 mM NaCl, 0.5 mM match-out deuterated DDM, pH: 3
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Aug 22
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
...Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 3.8 nm
Dmax 12.7 nm
VolumePorod 279 nm3

SASDL53 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with continuous-flow SEC-SANS

Proton-gated ion channel experimental SAS data
Proton-gated ion channel Kratky plot
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 20
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
...Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 3.8 nm
Dmax 12.0 nm
VolumePorod 235 nm3

SASDL63 – Gloeobacter violaceus Ligand-Gated Ion Channel (GLIC) at pH 7.5 measured with cuvette-mode SANS

Proton-gated ion channel experimental SAS data
Proton-gated ion channel Kratky plot
Sample: Proton-gated ion channel pentamer, 183 kDa Gloeobacter violaceus (strain … protein
Buffer: D2O, 20 mM Tris, 150 mM NaCl, 0.5 mM matched-out deuterated DDM,, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2019 Jun 21
Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering Proceedings of the National Academy of Sciences 118(37):e2108006118 (2021)
...Johansen N, Martel A, Porcar L, Arleth L, Howard R, Lindahl E
RgGuinier 4.0 nm
Dmax 17.7 nm
VolumePorod 225 nm3

SASDDY5 – AMPA subtype ionotropic Glutamate receptor GluA2 in the resting state (apo), in stealth DDM detergents

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2017 Sep 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
...Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.0 nm
Dmax 17.9 nm
VolumePorod 396 nm3

SASDDZ5 – AMPA subtype ionotropic Glutamate receptor GluA2 in the AMPA bound state, in stealth DDM detergents, pH 7.5

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 1 mM AMPA, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2016 Oct 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
...Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.3 nm
Dmax 18.4 nm
VolumePorod 407 nm3

SASDD26 – AMPA subtype ionotropic Glutamate receptor GluA2 in the AMPA bound state, in stealth DDM detergents, pH 5.5

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 10 mM AMPA, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 5.5
Experiment: SANS data collected at KWS1, FRM2 on 2017 Sep 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
...Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.5 nm
Dmax 18.9 nm
VolumePorod 899 nm3

SASDD36 – AMPA subtype ionotropic Glutamate receptor GluA2 in the GYKI-53655 bound state, in stealth DDM detergents

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 1 mM GYKI-53655, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2016 Oct 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
...Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.3 nm
Dmax 18.6 nm
VolumePorod 384 nm3

SASDQP6 – Nanodisc with POPC and circularized membrane scaffolding protein (csMSP1E3D1)

circularized Membrane scaffolding protein 1 E3 D11-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: circularized Membrane scaffolding protein 1 E3 D1 , 62 kDa protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) None, lipid
Buffer: 20 mM Tris-HCl pH 7.5, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5
Circularized and solubility‐enhanced MSP s facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution The FEBS Journal 286(9):1734-1751 (2019)
Johansen N, Tidemand F, Nguyen T, Rand K, Pedersen M, Arleth L
RgGuinier 5.8 nm
Dmax 14.5 nm