Search

  
Advanced search  

7 hits found for Kawakami

SASDUC2 – TIP120 (designed based on Truncated Icosahedral Protein composed of 60-mer fusion proteins)

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsC-terminal split fragment of a single subunit of Truncated Isosahedral Protein composed of 60-mer fusion proteins experimental SAS data
DAMMIN model
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 360 kDa synthetic construct protein
C-terminal split fragment of a single subunit of Truncated Isosahedral Protein composed of 60-mer fusion proteins , 715 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
Fusion then fission: splitting and reassembly of an artificial fusion-protein nanocage. Chem Commun (Camb) (2024)
...Kawakami N, Arai R, Adachi N, Ikeda A, Senda T, Miyamoto K
RgGuinier 9.2 nm
Dmax 22.5 nm
VolumePorod 3480 nm3

SASDUD2 – mTIP120-Ba (metal-ion induced 120-mer complex of TIP120 mutant (K26E in C-terminal subunit) with Ba ions)

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsK26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins experimental SAS data
DAMMIN model
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 360 kDa synthetic construct protein
K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 857 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 4 mM BaCl2, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
Fusion then fission: splitting and reassembly of an artificial fusion-protein nanocage. Chem Commun (Camb) (2024)
...Kawakami N, Arai R, Adachi N, Ikeda A, Senda T, Miyamoto K
RgGuinier 9.4 nm
Dmax 22.4 nm
VolumePorod 3320 nm3

SASDUE2 – TIP120 mutant K26E in CoreC (C-terminal split fragment of a fusion protein that assembles to Truncated Icosahedral protein composed of TIP60 ) subunit with EDTA

N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteinsK26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins experimental SAS data
N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins Kratky plot
Sample: N-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 360 kDa synthetic construct protein
K26E mutant of C-terminal split fragment of a single subunit of Truncated Icosahedral Protein composed of 60-mer fusion proteins , 857 kDa synthetic construct protein
Buffer: 25 mM HEPES, 100 mM NaCl, 4 mM BaCl2, 32 mM EDTA, 5%(v/v) glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 Nov 27
Fusion then fission: splitting and reassembly of an artificial fusion-protein nanocage. Chem Commun (Camb) (2024)
...Kawakami N, Arai R, Adachi N, Ikeda A, Senda T, Miyamoto K
RgGuinier 4.7 nm
Dmax 13.9 nm
VolumePorod 110 nm3

SASDPV2 – original TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil experimental SAS data
DAMMIN model
Sample: Fusion protein of LSm and MyoX-coil , 1066 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23
Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions. J Am Chem Soc (2022)
...Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K
RgGuinier 9.1 nm
Dmax 22.7 nm

SASDPW2 – mTIP60-Ba (metal-ion induced 60-mer complex of TIP60 (K67E) mutant with barium ions)

TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions)Barium ion experimental SAS data
DAMMIN model
Sample: TIP60 (K67E) mutant (metal-ion induced 60-mer complex with barium ions) , 1066 kDa Artificial protein protein
Barium ion 0, 8 kDa
Buffer: 25 mM HEPES, 100 mM NaCl, 5% glycerol, 5 mM BaCl2, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23
Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions. J Am Chem Soc (2022)
...Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K
RgGuinier 9.6 nm
Dmax 21.8 nm

SASDPX2 – TIP60 (K67E) mutant (dimer) with EDTA

TIP60 (K67E) mutant with EDTA experimental SAS data
DAMMIN model
Sample: TIP60 (K67E) mutant with EDTA dimer, 36 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2021 May 23
Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions. J Am Chem Soc (2022)
...Kawakami N, Arai R, Adachi N, Moriya T, Kawasaki M, Miyamoto K
RgGuinier 3.7 nm
Dmax 12.0 nm

SASDDZ8 – Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil experimental SAS data
DAMMIN model
Sample: Fusion protein of LSm and MyoX-coil , 1066 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Nov 11
Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces. Angew Chem Int Ed Engl (2018)
Kawakami N, Kondo H, Matsuzawa Y, Hayasaka K, Nasu E, Sasahara K, Arai R, Miyamoto K
RgGuinier 9.4 nm
Dmax 23.0 nm