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32 hits found for Miller

SASDCA3 – Zinc finger and BTB domain-containing protein 38 (ZBTB38) complexed with methylated C-terminal ZBTB38 binding sequence

Zinc finger and BTB domain-containing protein 38methylated C-terminal ZBTB38 binding sequence experimental SAS data
MONSA model
Sample: Zinc finger and BTB domain-containing protein 38 monomer, 17 kDa Homo sapiens protein
methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment: SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Nov 11
The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
...Miller SA, Buck-Koehntop BA
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 50 nm3

SASDCB3 – Methylated C-terminal ZBTB38 binding sequence (mCZ38BS)

methylated C-terminal ZBTB38 binding sequence experimental SAS data
DAMMIN model
Sample: methylated C-terminal ZBTB38 binding sequence monomer, 17 kDa DNA
Buffer: 10 mM Tris, 1 mM tris(2-carboxy-ethyl)phosphine (TCEP), 0.05% NaN3, 10% D2O, pH: 6.8
Experiment: SAXS data collected at Anton Paar SAXSess, Department of Chemistry, University of Utah on 2016 Oct 27
The C-Terminal Zinc Fingers of ZBTB38 are Novel Selective Readers of DNA Methylation. J Mol Biol 430(3):258-271 (2018)
...Miller SA, Buck-Koehntop BA
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 23 nm3

SASDMK3 – Mycobacterium phage TipsytheTRex apo protein

Immunity repressor experimental SAS data
BILBOMD model
Sample: Immunity repressor monomer, 24 kDa Mycobacterium phage TipsytheTRex protein
Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10
A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
...Miller JM, Gainey MD, Wallen JR
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 43 nm3

SASDML3 – Mycobacterium phage TipsytheTRex immunity repressor bound to 24mer DNA

Immunity repressor24mer dsDNA experimental SAS data
BILBOMD model
Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein
24mer dsDNA monomer, 15 kDa DNA
Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10
A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
...Miller JM, Gainey MD, Wallen JR
RgGuinier 2.4 nm
Dmax 9.0 nm
VolumePorod 43 nm3

SASDMM3 – Mycobacterium phage TipsytheTRex immunity repressor mixed with 13mer DNA

Immunity repressor13mer dsDNA experimental SAS data
BILBOMD model
Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein
13mer dsDNA monomer, 8 kDa DNA
Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 10
A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
...Miller JM, Gainey MD, Wallen JR
RgGuinier 2.0 nm
Dmax 8.5 nm
VolumePorod 18 nm3

SASDHR4 – A structural characterization of poly(aspartic acid) hydrolase-1 from Sphingomonas sp. KT-1

Poly(Aspartic acid) hydrolase-1 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Poly(Aspartic acid) hydrolase-1 dimer, 65 kDa Sphingomonas sp. KT-1 protein
Buffer: 20 mM Tris pH 7.0, 100 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27
Structural Characterization of Sphingomonas sp. KT-1 PahZ1-Catalyzed Biodegradation of Thermally Synthesized Poly(aspartic acid) ACS Sustainable Chemistry & Engineering (2020)
...Miller J, Wallen J, Weiland M
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 66 nm3

SASDP47 – Glucose Isomerase at a neutral pH and 5 MPa (1 atm) of pressure

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 25 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 253 nm3

SASDP57 – Glucose Isomerase at a neutral pH and 100 MPa (1000 atm) of pressure

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 3% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.5 nm
Dmax 10.6 nm
VolumePorod 267 nm3

SASDP67 – Bovine serum albumin at neutral pH, 4 °C, and 5 MPa (1 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.7 nm
Dmax 8.5 nm
VolumePorod 99 nm3

SASDP77 – Bovine serum albumin at neutral pH, 4 °C, and 100 MPa (1000 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 94 nm3

SASDP87 – Bovine serum albumin at neutral pH, 50 °C, and 5 MPa (1 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 108 nm3

SASDP97 – Bovine serum albumin at neutral pH, 50 °C, and 100 MPa (1000 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 104 nm3

SASDPA7 – Acidic leucine-rich nuclear phosphoprotein 32 family member A (pp32) at 4 °C and 5 MPa (1 atm)

Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) experimental SAS data
Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) Kratky plot
Sample: Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) monomer, 29 kDa Homo sapiens protein
Buffer: 25 mM BisTRIS, 10 mM NaCl, 5 mM DTT, pH: 6.8
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 1.8 nm
Dmax 6.0 nm
VolumePorod 31 nm3

SASDPB7 – Acidic leucine-rich nuclear phosphoprotein 32 family member A (pp32) at 4 °C and 100 MPa (1000 atm)

Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) experimental SAS data
Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) Kratky plot
Sample: Acidic leucine-rich nuclear phosphoprotein 32 family member A (L60A mutant) monomer, 29 kDa Homo sapiens protein
Buffer: 25 mM BisTRIS, 10 mM NaCl, 5 mM DTT, pH: 6.8
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.0 nm
Dmax 7.5 nm
VolumePorod 37 nm3

SASDLS7 – Mycobacterium phage TipsytheTRex immunity repressor bound to DNA

Immunity repressor21mer dsDNA experimental SAS data
BILBOMD model
Sample: Immunity repressor monomer, 21 kDa Mycobacterium phage TipsytheTRex protein
21mer dsDNA monomer, 13 kDa DNA
Buffer: 20 mM Tris pH 7.5, 0.5 M NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10
A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence. Nat Commun 13(1):4105 (2022)
...Miller JM, Gainey MD, Wallen JR
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 41 nm3

SASDJV4 – X-ray repair cross-complementing proteins 5ΔCTR and 6

X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5 ΔCTR experimental SAS data
BILBOMD model
Sample: X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein
X-ray repair cross-complementing protein 5 ΔCTR monomer, 64 kDa Homo sapiens protein
Buffer: 50 mM Hepes, 50 mM KCl, 5 mM MgCl2, 5% glycerol and 0.2 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 12
Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
...Miller SP, Tainer JA
RgGuinier 3.8 nm
Dmax 12.5 nm
VolumePorod 240 nm3

SASDG76 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)Serine/threonine-protein phosphatase PP1-alpha catalytic subunit experimental SAS data
BILBOMD model
Sample: Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 May 31
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Miller SP, Tainer JA, Holmes CFB, Glover JNM
RgGuinier 3.1 nm
Dmax 11.3 nm
VolumePorod 157 nm3

SASDNV7 – Obscurin Ig domains 11/12 at neutral pH

Obscurin experimental SAS data
Obscurin Kratky plot
Sample: Obscurin monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Nov 18
The N-terminus of obscurin is flexible in solution. Proteins (2022)
...Miller CJ, Wright NT
RgGuinier 3.7 nm
Dmax 18.0 nm
VolumePorod 66 nm3

SASDJU4 – X-ray repair cross-complementing proteins 5 and 6

X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5 experimental SAS data
BILBOMD model
Sample: X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein
X-ray repair cross-complementing protein 5 monomer, 83 kDa Homo sapiens protein
Buffer: 50 mM Hepes, 50 mM KCl, 5 mM MgCl2, 5% glycerol and 0.2 mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 12
Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
...Miller SP, Tainer JA
RgGuinier 4.2 nm
Dmax 14.9 nm
VolumePorod 253 nm3

SASDG86 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended

Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)Serine/threonine-protein phosphatase PP1-alpha catalytic subunit experimental SAS data
BILBOMD model
Sample: Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 May 31
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Miller SP, Tainer JA, Holmes CFB, Glover JNM
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 180 nm3

SASDNU7 – Obscurin Ig domains 12/13 at neutral pH

Obscurin experimental SAS data
Obscurin Kratky plot
Sample: Obscurin monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 18
The N-terminus of obscurin is flexible in solution. Proteins (2022)
...Miller CJ, Wright NT
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 28 nm3

SASDJW4 – X-ray repair cross-complementing proteins 5 and 6-DNA

X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5Y-DNA experimental SAS data
BILBOMD model
Sample: X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein
X-ray repair cross-complementing protein 5 monomer, 83 kDa Homo sapiens protein
Y-DNA monomer, 18 kDa DNA
Buffer: 50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 0.01% sodium azide, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Jan 8
Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
...Miller SP, Tainer JA
RgGuinier 4.1 nm
Dmax 14.8 nm
VolumePorod 280 nm3

SASDG66 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

Serine/threonine-protein phosphatase PP1-alpha catalytic subunitInhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 25
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Miller SP, Tainer JA, Holmes CFB, Glover JNM
RgGuinier 3.0 nm
Dmax 10.9 nm
VolumePorod 116 nm3

SASDNW7 – Obscurin Ig domains 12/13, short at neutral pH

Obscurin Ig domains 12/13 short experimental SAS data
MULTIFOXS model
Sample: Obscurin Ig domains 12/13 short monomer, 21 kDa Homo sapiens protein
Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 20
The N-terminus of obscurin is flexible in solution. Proteins (2022)
...Miller CJ, Wright NT
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 25 nm3

SASDJX4 – DNA-dependent protein kinase catalytic subunit (DNA-PKcs)

DNA-dependent protein kinase catalytic subunit experimental SAS data
BILBOMD model
Sample: DNA-dependent protein kinase catalytic subunit monomer, 468 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 0.01% sodium azide, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 30
Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
...Miller SP, Tainer JA
RgGuinier 5.6 nm
Dmax 15.6 nm
VolumePorod 1040 nm3

SASDG56 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended

Serine/threonine-protein phosphatase PP1-alpha catalytic subunitInhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 25
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Miller SP, Tainer JA, Holmes CFB, Glover JNM
RgGuinier 3.4 nm
Dmax 12.4 nm
VolumePorod 132 nm3

SASDNX7 – Obscurin Ig domains 12/13, long at neutral pH

Obscurin Ig domains 12/13 long experimental SAS data
MULTIFOXS model
Sample: Obscurin Ig domains 12/13 long monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Tris, 50 mM NaCl, 0.35 mM NaN3, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Oct 20
The N-terminus of obscurin is flexible in solution. Proteins (2022)
...Miller CJ, Wright NT
RgGuinier 2.9 nm
Dmax 10.0 nm
VolumePorod 26 nm3

SASDJY4 – Autophosphorylated DNA-dependent protein kinase (DNA-PKcs)

DNA-dependent protein kinase catalytic subunit experimental SAS data
BILBOMD model
Sample: DNA-dependent protein kinase catalytic subunit monomer, 468 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 100 mM KCl, 5% (v/v) glycerol, 0.2 mM EDTA containing 0.1 mM benzamidine, 0.2 mM PMSF and 0.2 µg/ml pepstatin, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Jan 8
Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
...Miller SP, Tainer JA
RgGuinier 5.5 nm
Dmax 16.1 nm
VolumePorod 918 nm3

SASDG96 – Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

Serine/threonine-protein phosphatase PP1-alpha catalytic subunitApoptosis-stimulating of p53 protein 2 experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Apoptosis-stimulating of p53 protein 2 monomer, 26 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 31
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Miller SP, Tainer JA, Holmes CFB, Glover JNM
RgGuinier 2.9 nm
Dmax 10.2 nm
VolumePorod 144 nm3

SASDJZ4 – DNA-dependent protein kinase/ X-ray repair cross-complementing protein 5 and 6 complex bound to DNA (DNA-PK monomer )

X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5DNA-dependent protein kinase catalytic subunitdsDNA experimental SAS data
BILBOMD model
Sample: X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein
X-ray repair cross-complementing protein 5 monomer, 83 kDa Homo sapiens protein
DNA-dependent protein kinase catalytic subunit monomer, 468 kDa Homo sapiens protein
dsDNA dimer, 21 kDa DNA
Buffer: 50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 0.01% sodium azide, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 30
Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
...Miller SP, Tainer JA
RgGuinier 6.5 nm
Dmax 23.1 nm
VolumePorod 1090 nm3

SASDGA6 – Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended

Serine/threonine-protein phosphatase PP1-alpha catalytic subunitApoptosis-stimulating of p53 protein 2 experimental SAS data
BILBOMD model
Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein
Apoptosis-stimulating of p53 protein 2 monomer, 26 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 31
Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis. Structure 27(10):1485-1496.e4 (2019)
...Miller SP, Tainer JA, Holmes CFB, Glover JNM
RgGuinier 3.3 nm
Dmax 11.7 nm
VolumePorod 172 nm3

SASDJ25 – DNA-dependent protein kinase/ X-ray repair cross-complementing protein 5 and 6 complex bound to DNA (DNA-PK monomer/dimer)

X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5DNA-dependent protein kinase catalytic subunitdsDNA experimental SAS data
BILBOMD model
Sample: X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein
X-ray repair cross-complementing protein 5 monomer, 83 kDa Homo sapiens protein
DNA-dependent protein kinase catalytic subunit monomer, 468 kDa Homo sapiens protein
dsDNA dimer, 21 kDa DNA
Buffer: 50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 0.01% sodium azide, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 30
Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
...Miller SP, Tainer JA
RgGuinier 7.5 nm
Dmax 29.4 nm
VolumePorod 1440 nm3