Small Angle Scattering Biological Data Bank SASBDB


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8 hits found for Morishima

SASDNJ2 – Circadian clock KaiA-KaiB-KaiC ternary protein complex measured by SEC-SAXS

Circadian clock protein KaiACircadian clock protein KaiBCircadian clock protein kinase KaiC (S431D mutant) experimental SAS data

Sample:	Circadian clock protein KaiA dodecamer, 393 kDa Synechococcus elongatus (strain … protein Circadian clock protein KaiB hexamer, 71 kDa Synechococcus elongatus (strain … protein Circadian clock protein kinase KaiC (S431D mutant) hexamer, 357 kDa Synechococcus elongatus (strain … protein
Buffer:	50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, pH: 7.8
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Dec 8

Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach. Commun Biol 5(1):184 (2022)
...Morishima K, Martel A, Porcar L, Sato N, Yogo R, Tominaga T, Inoue R, Yagi-Utsumi M, Okuda A, Shimizu M, Urade R, Terauchi K, Kono H, Yagi H, Kato K, Sugiyama M

R_g^Guinier	7.0	nm
D_max	24.5	nm
Volume^Porod	1650	nm³

SASDNK2 – Circadian clock hKaiA-75dKaiB-75dKaiC ternary protein complex measured by SEC-SANS in 100% D2O

75% deuterated Circadian clock protein KaiBCircadian clock protein KaiA75% deuterated Circadian clock protein kinase KaiC (S431D mutant) experimental SAS data

Sample:	75% deuterated Circadian clock protein KaiB hexamer, 71 kDa Synechococcus elongatus (strain … protein Circadian clock protein KaiA dodecamer, 393 kDa Synechococcus elongatus (strain … protein 75% deuterated Circadian clock protein kinase KaiC (S431D mutant) hexamer, 357 kDa Synechococcus elongatus (strain … protein
Buffer:	50 mM sodium phosphate buffer, 150 mm NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D2O, pH: 7.8
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Sep 19

R_g^Guinier	7.8	nm
D_max	25.6	nm
Volume^Porod	1620	nm³

SASDWA3 – Ovalbumin, Monomer from SEC-SANS

Sample:	Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer:	100 mM Tris-HCl, 100 mM NaCl in 100% v/v D₂O, pH: 7.5
Experiment:	SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Apr 22

Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux Journal of Applied Crystallography 58(2) (2025)
Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M

R_g^Guinier	2.0	nm
D_max	6.5	nm

SASDWB3 – Bovine serum albumin, monomer from SEC-SANS

Albumin (natural variant A214T) experimental SAS data

Sample:	Albumin (natural variant A214T) monomer, 66 kDa Bos taurus protein
Buffer:	100 mM Tris-HCl, 100 mM NaCl in 100% v/v D₂O, pH: 7.5
Experiment:	SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Apr 22

R_g^Guinier	2.6	nm
D_max	8.7	nm

SASDWC3 – Apoferritin, from SEC-SANS

Ferritin light chain experimental SAS data

Sample:	Ferritin light chain 24-mer, 476 kDa Equus caballus protein
Buffer:	100 mM Tris-HCl, 100 mM NaCl in 100% v/v D₂O, pH: 7.5
Experiment:	SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Apr 22

R_g^Guinier	5.4	nm
D_max	23.1	nm

SASDWD3 – Cyanobacterial circadian clock protein KaiB-KaiC complex (hydrogenated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiBCircadian clock oscillator protein KaiC experimental SAS data

Circadian clock oscillator protein KaiB Circadian clock oscillator protein KaiC Kratky plot

Sample:	Circadian clock oscillator protein KaiB hexamer, 71 kDa Synechococcus sp. (strain … protein Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer:	50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment:	SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2

R_g^Guinier	4.9	nm
D_max	14.4	nm

SASDWE3 – Cyanobacterial circadian clock protein KaiB-KaiC complex (71% deuterated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Sample:	Circadian clock oscillator protein KaiB hexamer, 71 kDa Synechococcus sp. (strain … protein Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer:	50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment:	SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2

R_g^Guinier	4.3	nm
D_max	11.7	nm

SASDWF3 – Circadian clock protein KaiC (hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiC experimental SAS data

Circadian clock oscillator protein KaiC Kratky plot

Sample:	Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer:	50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment:	SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2

R_g^Guinier	4.4	nm
D_max	11.9	nm

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