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2 hits found for Paissoni

SASDKH2 – E2F1/DP1 in complex with a 15-mer DNA oligo

Transcription factor E2F115-mer DNA oligo of the human E2F1 promoterE2F dimerization partner 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Transcription factor E2F1 monomer, 20 kDa Homo sapiens protein
15-mer DNA oligo of the human E2F1 promoter monomer, 9 kDa DNA
E2F dimerization partner 1 monomer, 29 kDa Homo sapiens protein
Buffer: 50 mM Tris-Cl pH 8.0, 150 mM NaCl and 2 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 19
High Conformational Flexibility of the E2F1/DP1/DNA Complex. J Mol Biol 433(18):167119 (2021)
...Paissoni C, Chaves-Sanjuan A, Nardini M, Mantovani R, Gnesutta N, Camilloni C
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 99 nm3

SASDSN7 – Full length human mature gelsolin in closed state

Gelsolin experimental SAS data
GROMACS model
Sample: Gelsolin monomer, 85 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Feb 24
Accurate and Efficient SAXS/SANS Implementation Including Solvation Layer Effects Suitable for Molecular Simulations. J Chem Theory Comput (2023)
...Paissoni C, Bollati M, de Rosa M, Capelli R, Camilloni C
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 128 nm3