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19 hits found for Sauter

SASDDF7 – Armless mitochondrial tRNA-Arg from Romanomermis culicivorax

Arginyl transfer RNA experimental SAS data
PYMOL model
Sample: Arginyl transfer RNA monomer, 13 kDa Romanomermis culicivorax RNA
Buffer: 50 mM HEPES-NaOH 10 mM MgCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Dec 16
Small but large enough: structural properties of armless mitochondrial tRNAs from the nematode Romanomermis culicivorax. Nucleic Acids Res (2018)
...Sauter C, Mörl M
RgGuinier 2.1 nm
Dmax 6.7 nm
VolumePorod 20 nm3

SASDDG7 – Armless mitochondrial tRNA-Ile from Romanomermis culicivorax

Isoleucyl transfer RNA experimental SAS data
PYMOL model
Sample: Isoleucyl transfer RNA monomer, 15 kDa Romanomermis culicivorax RNA
Buffer: 50 mM HEPES-NaOH 10 mM MgCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Dec 16
Small but large enough: structural properties of armless mitochondrial tRNAs from the nematode Romanomermis culicivorax. Nucleic Acids Res (2018)
...Sauter C, Mörl M
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 20 nm3

SASDQ47 – N-terminal GST-like domain of Plasmodium berghei tRNA import protein

tRNA import protein tRIP experimental SAS data
CORAL model
Sample: tRNA import protein tRIP dimer, 49 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
...Sauter C, Frugier M
RgGuinier 2.9 nm
Dmax 11.0 nm
VolumePorod 68 nm3

SASDUL9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 11.6 nm
VolumePorod 23 nm3

SASDQ37 – N-terminal GST-like domain of Plasmodium berghei cytosolic glutamyl-tRNA synthetase

Glutamate--tRNA ligase experimental SAS data
OLIGOMER model
Sample: Glutamate--tRNA ligase dimer, 57 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
...Sauter C, Frugier M
RgGuinier 3.0 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDUM9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 13.8 nm
VolumePorod 18 nm3

SASDQ57 – The scaffold of Plasmodium berghei multi aminoacyl-tRNA synthetase complexes

Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase experimental SAS data
CORAL model
Sample: Chimeric construct containing the GST-like domains of P. berghei tRNA import protein and glutamyl-tRNA synthetase dimer, 105 kDa Plasmodium berghei ANKA protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
...Sauter C, Frugier M
RgGuinier 3.9 nm
Dmax 14.1 nm
VolumePorod 145 nm3

SASDUQ9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.8 nm
VolumePorod 22 nm3

SASDQ67 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, Q-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and glutaminyl-tRNA synthetase

Glutamine--tRNA ligaseGlutamate--tRNA ligasetRNA import protein tRIP experimental SAS data
CORAL model
Sample: Glutamine--tRNA ligase dimer, 46 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
tRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
...Sauter C, Frugier M
RgGuinier 4.6 nm
Dmax 17.1 nm
VolumePorod 198 nm3

SASDUP9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.0 nm
Dmax 14.4 nm
VolumePorod 31 nm3

SASDQ77 – Plasmodium berghei multi aminoacyl-tRNA synthetase complex, M-complex containing the GST-like domains of tRNA import protein, glutamyl-tRNA synthetase and methionyl-tRNA synthetase

Methionine--tRNA ligasetRNA import protein tRIPGlutamate--tRNA ligase experimental SAS data
CORAL model
Sample: Methionine--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
tRNA import protein tRIP dimer, 48 kDa Plasmodium berghei (strain … protein
Glutamate--tRNA ligase dimer, 55 kDa Plasmodium berghei (strain … protein
Buffer: 25 mM HEPES-NaOH, 300 mM NaCl, 5% (v/v) glycerol, 0.005% (m/v) n-dodecyl beta-D-maltoside, 5 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 9
Solution x-ray scattering highlights discrepancies in Plasmodium multi-aminoacyl-tRNA synthetase complexes. Protein Sci :e4564 (2023)
...Sauter C, Frugier M
RgGuinier 4.7 nm
Dmax 18.3 nm
VolumePorod 217 nm3

SASDUN9 – Wild-type Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 29
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.9 nm
VolumePorod 23 nm3

SASDUR9 – Br-labelled Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.0 nm
Dmax 12.3 nm
VolumePorod 28 nm3

SASDUS9 – Wild-type Elastin-like Polypeptide (ELP) in 0.5 M Urea

Elastin-like polypeptide experimental SAS data
Elastin-like polypeptide Kratky plot
Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 10.7 nm
VolumePorod 47 nm3

SASDUT9 – Br-labelled Elastin-like Polypeptide (ELP) in 0.5 M Urea

Elastin-like polypeptide labeled with unnatural amino acid diBrK experimental SAS data
Elastin-like polypeptide labeled with unnatural amino acid diBrK Kratky plot
Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 18.4 nm
VolumePorod 43 nm3

SASDUV9 – Wild-type Elastin-like Polypeptide (ELP) in 2 M Urea

Elastin-like polypeptide experimental SAS data
Elastin-like polypeptide Kratky plot
Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.5 nm
Dmax 18.0 nm
VolumePorod 53 nm3

SASDUU9 – Br-labelled Elastin-like Polypeptide (ELP) in 2 M Urea

Elastin-like polypeptide labeled with unnatural amino acid diBrK experimental SAS data
Elastin-like polypeptide labeled with unnatural amino acid diBrK Kratky plot
Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.5 nm
Dmax 14.8 nm
VolumePorod 48 nm3

SASDUW9 – Wild-type Elastin-like Polypeptide (ELP) in 6 M Urea

Elastin-like polypeptide experimental SAS data
Elastin-like polypeptide Kratky plot
Sample: Elastin-like polypeptide monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.7 nm
Dmax 21.7 nm
VolumePorod 52 nm3

SASDUX9 – Br-labelled Elastin-like Polypeptide (ELP) in 6 M Urea

Elastin-like polypeptide labeled with unnatural amino acid diBrK experimental SAS data
Elastin-like polypeptide labeled with unnatural amino acid diBrK Kratky plot
Sample: Elastin-like polypeptide labeled with unnatural amino acid diBrK monomer, 13 kDa synthetic construct protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 2
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
...Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.8 nm
Dmax 18.1 nm
VolumePorod 47 nm3