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8 hits found for Schanda

SASDEF2 – Mitochondrial import inner membrane translocase complex TIM9·10

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CORAL model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Tris, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDEG2 – Mitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial GTP/GDP carrier protein 1 experimental SAS data
DAMMIF model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 hexamer, 61 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 hexamer, 61 kDa Saccharomyces cerevisiae protein
Mitochondrial GTP/GDP carrier protein 1 monomer, 33 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Tris, 150mM NaCl, imidiazole, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Schanda P
RgGuinier 4.5 nm
Dmax 16.0 nm
VolumePorod 272 nm3

SASDD93 – ATP-dependent Clp protease ATP-binding subunit ClpC1

ATP-dependent Clp protease ATP-binding subunit ClpC1 experimental SAS data
OTHER model
Sample: ATP-dependent Clp protease ATP-binding subunit ClpC1 , 95 kDa Mycobacterium tuberculosis protein
Buffer: Hepes 50 mM pH 7.5, KCl 100 mM, glycerol 10%, MgCl2 4 mM and ATP 1 mM, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 18
The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis. J Biol Chem 293(22):8379-8393 (2018)
...Schanda P, Fraga H
RgGuinier 7.6 nm
Dmax 25.0 nm
VolumePorod 2156 nm3

SASDDA3 – ATP-dependent Clp protease ATP-binding subunit ClpC1, second state

ATP-dependent Clp protease ATP-binding subunit ClpC1 experimental SAS data
ATP-dependent Clp protease ATP-binding subunit ClpC1 Kratky plot
Sample: ATP-dependent Clp protease ATP-binding subunit ClpC1 , 95 kDa Mycobacterium tuberculosis protein
Buffer: Hepes 50 mM pH 7.5, KCl 100 mM, glycerol 10%, MgCl2 4 mM and ATP 1 mM, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 18
The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis. J Biol Chem 293(22):8379-8393 (2018)
...Schanda P, Fraga H
RgGuinier 7.9 nm
Dmax 25.1 nm
VolumePorod 2416 nm3

SASDH28 – Mitochondrial import inner membrane translocase TIM 9·10·12

Mitochondrial import inner membrane translocase subunit TIM12Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM12 monomer, 12 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM9 dimer, 20 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM MES buffer (2-(N-morpholino)ethanesulfonic acid), pH 6.5, 50 mM NaCl Cloning,, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2017 May 8
Architecture and subunit dynamics of the mitochondrial TIM9·10·12 chaperone (2020)
...Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDH89 – Mitochondrial TIM8·13 chaperone in complex with the Tim23 membrane protein precursor

Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13Mitochondrial import inner membrane translocase subunit TIM23 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 24
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Schanda P
RgGuinier 3.1 nm
Dmax 11.3 nm

SASDJP4 – Mitochondrial import inner membrane translocase subunits TIM9-TIM10 in complex with TIM23

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial import inner membrane translocase subunit TIM23 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 May 18
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Schanda P
RgGuinier 3.2 nm
Dmax 7.7 nm
VolumePorod 146 nm3

SASDJQ4 – Mitochondrial import inner membrane translocase subunits TIM8-TIM13

Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Schanda P
RgGuinier 3.1 nm
Dmax 10.9 nm
VolumePorod 136 nm3