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4 hits found for Selmer

SASDJ55 – S-adenosylmethionine (SAM) lyase Svi3-3

Phage-encoded SAM lyase Svi3-3 (including N-terminal His6-tag and Tev cleavage site) experimental SAS data
Phage-encoded SAM lyase Svi3-3 (including N-terminal His6-tag and Tev cleavage site) Kratky plot
Sample: Phage-encoded SAM lyase Svi3-3 (including N-terminal His6-tag and Tev cleavage site) trimer, 56 kDa Unknown environmental phage protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Dec 9
Structure and mechanism of a phage-encoded SAM lyase revises catalytic function of enzyme family. Elife 10 (2021)
...Selmer M
RgGuinier 2.5 nm
Dmax 9.3 nm
VolumePorod 85 nm3

SASDJ65 – S-adenosylmethionine (SAM) lyase Svi3-3 in presence of SAM

Phage-encoded SAM lyase Svi3-3 (including N-terminal His6-tag and Tev cleavage site) experimental SAS data
Phage-encoded SAM lyase Svi3-3 (including N-terminal His6-tag and Tev cleavage site) Kratky plot
Sample: Phage-encoded SAM lyase Svi3-3 (including N-terminal His6-tag and Tev cleavage site) trimer, 56 kDa Unknown environmental phage protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 5 mM SAM, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Dec 9
Structure and mechanism of a phage-encoded SAM lyase revises catalytic function of enzyme family. Elife 10 (2021)
...Selmer M
RgGuinier 2.5 nm
Dmax 9.1 nm
VolumePorod 89 nm3

SASDQ95 – The complex formed between E. coli S-adenosylmethionine synthase and wild-type S-adenosyl-L-methionine lyase from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Jun 17
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
...Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.5 nm
VolumePorod 325 nm3

SASDQA5 – The complex formed between E. coli S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase (E68Q/Q94A) experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase (E68Q/Q94A) tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 May 7
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
...Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 326 nm3