Small Angle Scattering Biological Data Bank SASBDB


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7 hits found for Tosi

SASDE78 – Solution structure of the diadenylate cyclase/phosphoglucosamine mutase (DacA/GlmM) complex from Staphylococcus aureus

Diadenylate cyclasePhosphoglucosamine mutase experimental SAS data

Sample:	Diadenylate cyclase dimer, 39 kDa Staphylococcus aureus protein Phosphoglucosamine mutase dimer, 99 kDa Staphylococcus aureus protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 7

Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM. PLoS Pathog 15(1):e1007537 (2019)
Tosi T, Hoshiga F, Millership C, Singh R, Eldrid C, Patin D, Mengin-Lecreulx D, Thalassinos K, Freemont P, Gründling A

R_g^Guinier	3.9	nm
D_max	12.1	nm
Volume^Porod	204	nm³

SASDE88 – Solution structure of phosphoglucosamine mutase (GlmM) from Staphylococcus aureus

Phosphoglucosamine mutase experimental SAS data

Sample:	Phosphoglucosamine mutase dimer, 99 kDa Staphylococcus aureus protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 7

R_g^Guinier	3.7	nm
D_max	12.5	nm
Volume^Porod	134	nm³

SASDE98 – Solution structure of Diadenylate cyclase (DacA) from Staphylococcus aureus

Diadenylate cyclase experimental SAS data

Sample:	Diadenylate cyclase dimer, 39 kDa Staphylococcus aureus protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 7

R_g^Guinier	2.6	nm
D_max	8.6	nm
Volume^Porod	57	nm³

SASDL25 – Bacillus subtilis complex of diadenylate cyclase (CdaA:cytoplasmic domain) and phosphoglucosamine mutase (GlmM)

Cyclic di-AMP synthase CdaAPhosphoglucosamine mutase experimental SAS data

Cyclic di-AMP synthase CdaA Phosphoglucosamine mutase Kratky plot

Sample:	Cyclic di-AMP synthase CdaA dimer, 44 kDa Bacillus subtilis (strain … protein Phosphoglucosamine mutase dimer, 101 kDa Bacillus subtilis (strain … protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 21

Structural basis for the inhibition of the Bacillus subtilis c-di-AMP cyclase CdaA by the phosphoglucomutase GlmM Journal of Biological Chemistry :101317 (2021)
...Tosi T, Millership C, Hoshiga F, Morgan R, Freemont P, Gründling A

R_g^Guinier	4.5	nm
D_max	16.2	nm
Volume^Porod	250	nm³

SASDMQ5 – Bacillus subtilis complex of diadenylate cyclase (CdaA:cytoplasmic domain) in complex with a truncated version of phosphoglucosamine mutase (GlmMF369)

Sample:	Cyclic di-AMP synthase CdaA dimer, 44 kDa Bacillus subtilis (strain … protein Phosphoglucosamine mutase dimer, 83 kDa Bacillus subtilis protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Aug 2

R_g^Guinier	3.7	nm
D_max	15.1	nm
Volume^Porod	156	nm³

SASDLZ4 – Bacillus subtilis phosphoglucosmine mutase GlmM

Sample:	Phosphoglucosamine mutase dimer, 101 kDa Bacillus subtilis (strain … protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 21

R_g^Guinier	3.7	nm
D_max	12.2	nm
Volume^Porod	140	nm³

SASDLY4 – Bacillus subtilis diadenylate cyclase CdaA: cytoplasmic domain

Cyclic di-AMP synthase CdaA experimental SAS data

Sample:	Cyclic di-AMP synthase CdaA dimer, 44 kDa Bacillus subtilis (strain … protein
Buffer:	30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Jul 21

R_g^Guinier	2.6	nm
D_max	8.9	nm
Volume^Porod	60	nm³