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17 hits found for Wen

SASDR32 – Human derived autoantibody mAb2G7 at pH 8.0

Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VHHuman derived autoantibody mAb2G7 light chain, mAb2G7 VL experimental SAS data
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL Kratky plot
Sample: Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
Buffer: phosphate buffered saline, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Wen H, Zhang J, Zhou Q, Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 5.0 nm
Dmax 16.0 nm
VolumePorod 260 nm3

SASDR42 – Human derived autoantibody mAb5F6 at pH 8.0

Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VHHuman derived autoantibody mAb2G7 light chain, mAb2G7 VL experimental SAS data
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL Kratky plot
Sample: Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
Buffer: phosphate buffered saline, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Wen H, Zhang J, Zhou Q, Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 5.0 nm
Dmax 15.8 nm
VolumePorod 252 nm3

SASDR52 – Human GluN1-GluN2A NMDA receptor at pH 8.0

Glutamate receptor ionotropic, NMDA 1Glutamate receptor ionotropic, NMDA 2A experimental SAS data
Glutamate receptor ionotropic, NMDA 1 Glutamate receptor ionotropic, NMDA 2A Kratky plot
Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Wen H, Zhang J, Zhou Q, Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 6.6 nm
Dmax 20.4 nm
VolumePorod 1180 nm3

SASDR62 – Human GluN1-GluN2A NMDA receptor in complex with human derived autoantibody mAb2G7 at pH 8.0

Glutamate receptor ionotropic, NMDA 1Glutamate receptor ionotropic, NMDA 2AHuman derived autoantibody mAb2G7 heavy chain, mAb2G7 VHHuman derived autoantibody mAb2G7 light chain, mAb2G7 VL experimental SAS data
Glutamate receptor ionotropic, NMDA 1 Glutamate receptor ionotropic, NMDA 2A Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL Kratky plot
Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Wen H, Zhang J, Zhou Q, Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 7.7 nm
Dmax 25.4 nm
VolumePorod 1260 nm3

SASDR72 – Human GluN1-GluN2A NMDA receptor in complex with human derived autoantibody mAb5F6 at pH 8.0

Glutamate receptor ionotropic, NMDA 1Glutamate receptor ionotropic, NMDA 2AHuman derived autoantibody mAb5F6 heavy chain, mAb5F6 VHHuman derived autoantibody mAb5F6 light chain, mAb5F6 VL experimental SAS data
Glutamate receptor ionotropic, NMDA 1 Glutamate receptor ionotropic, NMDA 2A Human derived autoantibody mAb5F6 heavy chain, mAb5F6 VH Human derived autoantibody mAb5F6 light chain, mAb5F6 VL Kratky plot
Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
Human derived autoantibody mAb5F6 heavy chain, mAb5F6 VH dimer, 104 kDa Homo sapiens protein
Human derived autoantibody mAb5F6 light chain, mAb5F6 VL dimer, 52 kDa protein
Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Wen H, Zhang J, Zhou Q, Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 9.9 nm
Dmax 31.7 nm
VolumePorod 2500 nm3

SASDUL2 – mRNA capping enzyme small subunit

Virus termination factor small subunit experimental SAS data
DAMFILT model
Sample: Virus termination factor small subunit monomer, 33 kDa Monkeypox virus (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 Jan 4
Structural basis of the monkeypox virus mRNA cap N7 methyltransferase complex. Emerg Microbes Infect 13(1):2369193 (2024)
...Wen Y, Shen Y, Zhang Y, Zhang L, Zhao G, Ding J, Li J
RgGuinier 2.4 nm
Dmax 6.8 nm
VolumePorod 51 nm3

SASDUM2 – mRNA capping enzyme MTase

Virus termination factor small subunitmRNA-capping enzyme catalytic subunit experimental SAS data
DAMFILT model
Sample: Virus termination factor small subunit monomer, 33 kDa Monkeypox virus (strain … protein
mRNA-capping enzyme catalytic subunit monomer, 35 kDa Monkeypox virus (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 Jan 4
Structural basis of the monkeypox virus mRNA cap N7 methyltransferase complex. Emerg Microbes Infect 13(1):2369193 (2024)
...Wen Y, Shen Y, Zhang Y, Zhang L, Zhao G, Ding J, Li J
RgGuinier 2.9 nm
Dmax 10.1 nm
VolumePorod 106 nm3

SASDL35 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (12 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 6.6 nm
Dmax 23.3 nm
VolumePorod 320 nm3

SASDL45 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (6 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 6.6 nm
Dmax 22.9 nm
VolumePorod 325 nm3

SASDL55 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (4 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 6.4 nm
Dmax 21.9 nm
VolumePorod 315 nm3

SASDL65 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (3 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 6.3 nm
Dmax 19.9 nm
VolumePorod 315 nm3

SASDL75 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Tetramer analyzed by SVD-EFA SEC-SAXS

Survival motor neuron proteinMaltose/maltodextrin-binding periplasmic protein experimental SAS data
Survival motor neuron protein Maltose/maltodextrin-binding periplasmic protein Kratky plot
Sample: Survival motor neuron protein tetramer, 15 kDa Drosophila melanogaster protein
Maltose/maltodextrin-binding periplasmic protein tetramer, 174 kDa Escherichia coli (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 3.9 nm
Dmax 11.3 nm
VolumePorod 340 nm3

SASDL85 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Octamer analyzed by SVD-EFA SEC-SAXS

Maltose/maltodextrin-binding periplasmic proteinSurvival motor neuron protein experimental SAS data
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Kratky plot
Sample: Maltose/maltodextrin-binding periplasmic protein octamer, 347 kDa Escherichia coli (strain … protein
Survival motor neuron protein octamer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 5.6 nm
Dmax 17.9 nm
VolumePorod 800 nm3

SASDL95 – Drosophila Melanogaster Gemin2-SMN: Octamer analyzed by SVD-EFA SEC-SAXS

Protein Gemin2Survival motor neuron protein experimental SAS data
Protein Gemin2 Survival motor neuron protein Kratky plot
Sample: Protein Gemin2 octamer, 230 kDa Drosophila melanogaster protein
Survival motor neuron protein octamer, 197 kDa Drosophila melanogaster protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG
RgGuinier 7.8 nm
Dmax 28.7 nm
VolumePorod 2660 nm3

SASDK48 – Histidine kinase AdeS - DHp-CA Domain

Histidine kinase experimental SAS data
MULTIFOXS model
Sample: Histidine kinase dimer, 51 kDa Acinetobacter baumannii protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Apr 21
Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS. iScience 24(5):102476 (2021)
...Wen Y
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 85 nm3

SASDK58 – Histidine kinase AdeS - cytoplasmic domain

Histidine kinase experimental SAS data
OTHER model
Sample: Histidine kinase hexamer, 186 kDa Acinetobacter baumannii protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 19
Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS. iScience 24(5):102476 (2021)
...Wen Y
RgGuinier 4.6 nm
Dmax 16.3 nm
VolumePorod 378 nm3

SASDP69 – Acinetobacter baumannii carbonic anhydrase PaaY trimer

Bacterial transferase hexapeptide repeat protein experimental SAS data
CORAL model
Sample: Bacterial transferase hexapeptide repeat protein trimer, 66 kDa Acinetobacter baumannii (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jan 29
Mechanistic and structural insights into the bifunctional enzyme PaaY from Acinetobacter baumannii. Structure (2023)
...Wen Y
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 102 nm3