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12 hits found for Actin, cytoplasmic 1

SASDUX3 – F-actin in F-buffer at an actin concentration of 2 mg/mL

Actin, cytoplasmic 1 experimental SAS data
PYMOL model
Sample: Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: ...1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 13.4 nm
Dmax 48.0 nm

SASDUY3 – ...1:32 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: ...1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 12.2 nm

SASDUZ3 – ...1:16 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: ...1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 14.5 nm

SASDU24 – ...1:8 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: ...1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 13.6 nm

SASDU34 – ...1:4 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: ...1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 8.8 nm

SASDU44 – ...1:2 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: ...1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 7.3 nm

SASDU54 – ...1:1 molar ratio in F-actin buffer

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: ...1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 5.2 nm

SASDU64 – ...1:2 molar ratio from high to low ionic strength

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer: ...1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 4.7 nm
Dmax 25.0 nm
VolumePorod 262 nm3

SASDU74 – ...1:1 molar ratio from high to low ionic strength

GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer: ...1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 5.2 nm
Dmax 25.0 nm
VolumePorod 266 nm3

SASDU84 – ...1:2 molar ratio in low ionic strength (SEC-SAXS)

GelsolinActin, cytoplasmic 1 experimental SAS data
GASBOR model
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer: ...1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 4.4 nm
Dmax 25.0 nm
VolumePorod 241 nm3

SASDU94 – ...1:1 molar ratio in low ionic strength (SEC-SAXS)

GelsolinActin, cytoplasmic 1 experimental SAS data
GASBOR model
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer: ...1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...13 Sep 1
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 4.6 nm
Dmax 25.0 nm
VolumePorod 243 nm3

SASDFD6 – ...cytoplasmic 1 actin complex

Protein kinase YopOActin, cytoplasmic 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Protein kinase YopO monomer, 63 kDa Yersinia enterocolitica protein
Actin, cytoplasmic 1 monomer, 42 kDa Homo sapiens protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at ...12, PETRA III on ...17 May 6
Studying Conformational Changes of the Yersinia Type-III-Secretion Effector YopO in Solution by Integrative Structural Biology. Structure 27(9):1416-1426.e3 (2019)
Peter MF, Tuukkanen AT, Heubach CA, Selsam A, Duthie FG, Svergun DI, Schiemann O, Hagelueken G
RgGuinier 3.6 nm
Dmax 12.3 nm
VolumePorod 147 nm3