Small Angle Scattering Biological Data Bank SASBDB


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6 hits found for Bifunctional hemolysin/adenylate cyclase

SASDJS3 – ...cyclase toxin CyaA RTX block II-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 57 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L

R_g^Guinier	4.0	nm
D_max	13.3	nm
Volume^Porod	94	nm³

SASDJT3 – ...cyclase toxin CyaA RTX block III-V

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 45 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

R_g^Guinier	3.4	nm
D_max	12.1	nm
Volume^Porod	85	nm³

SASDJU3 – ...cyclase toxin CyaA RTX block IV-V

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 32 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

R_g^Guinier	2.7	nm
D_max	8.6	nm
Volume^Porod	45	nm³

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDCK9 – ...Cyclase Toxin (CyaA)

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A

R_g^Guinier	2.6	nm
D_max	9.1	nm
Volume^Porod	60	nm³

SASDCL9 – ...Cyclase Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19