Search

  
Advanced search  

4 hits found for Circadian clock protein KaiB

SASDNJ2 – Circadian clock KaiA-KaiB-KaiC ternary protein complex measured by SEC-SAXS

Circadian clock protein KaiACircadian clock protein KaiBCircadian clock protein kinase KaiC (S431D mutant) experimental SAS data
OTHER model
Sample: Circadian clock protein KaiA dodecamer, 393 kDa Synechococcus elongatus (strain … protein
Circadian clock protein KaiB hexamer, 71 kDa Synechococcus elongatus (strain … protein
Circadian clock protein kinase KaiC (S431D mutant) hexamer, 357 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, pH: 7.8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Dec 8
...circadian clock complex by an integrated experimental-computational approach. Commun Biol 5(1):184 (2022)
Yunoki Y, Matsumoto A, Morishima K, Martel A, Porcar L, Sato N, Yogo R, Tominaga T, Inoue R, Yagi-Utsumi M, Okuda A, Shimizu M, Urade R, Terauchi K, Kono H, Yagi H, Kato K, Sugiyama M
RgGuinier 7.0 nm
Dmax 24.5 nm
VolumePorod 1650 nm3

SASDNK2 – Circadian clock hKaiA-75dKaiB-75dKaiC ternary protein complex measured by SEC-SANS in 100% D2O

75% deuterated Circadian clock protein KaiBCircadian clock protein KaiA75% deuterated Circadian clock protein kinase KaiC (S431D mutant) experimental SAS data
OTHER model
Sample: ...Circadian clock protein KaiB hexamer, 71 kDa Synechococcus elongatus (strain … protein
Circadian clock protein KaiA dodecamer, 393 kDa Synechococcus elongatus (strain … protein
...Circadian clock protein kinase KaiC (S431D mutant) hexamer, 357 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mm NaCl, 5 mM MgCl2, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D2O, pH: 7.8
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Sep 19
...circadian clock complex by an integrated experimental-computational approach. Commun Biol 5(1):184 (2022)
Yunoki Y, Matsumoto A, Morishima K, Martel A, Porcar L, Sato N, Yogo R, Tominaga T, Inoue R, Yagi-Utsumi M, Okuda A, Shimizu M, Urade R, Terauchi K, Kono H, Yagi H, Kato K, Sugiyama M
RgGuinier 7.8 nm
Dmax 25.6 nm
VolumePorod 1620 nm3

SASDWD3 – ...circadian clock protein KaiB-KaiC complex (hydrogenated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiBCircadian clock oscillator protein KaiC experimental SAS data
Circadian clock oscillator protein KaiB Circadian clock oscillator protein KaiC Kratky plot
Sample: Circadian clock oscillator protein KaiB hexamer, 71 kDa Synechococcus sp. (strain … protein
Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2
Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux Journal of Applied Crystallography 58(2) (2025)
Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M
RgGuinier 4.9 nm
Dmax 14.4 nm

SASDWE3 – ...circadian clock protein KaiB-KaiC complex (71% deuterated-KaiB and hydrogenated-KaiC in 100% v/v D₂O ) measured by SEC-SANS

Circadian clock oscillator protein KaiBCircadian clock oscillator protein KaiC experimental SAS data
Circadian clock oscillator protein KaiB Circadian clock oscillator protein KaiC Kratky plot
Sample: Circadian clock oscillator protein KaiB hexamer, 71 kDa Synechococcus sp. (strain … protein
Circadian clock oscillator protein KaiC hexamer, 356 kDa Synechococcus elongatus (strain … protein
Buffer: 50 mM sodium phosphate buffer, 150 mM NaCl, 5 mM MgCl₂, 0.5 mM EDTA, 1 mM ATP, 1 mM DTT, 50 mM arginine, 50 mM glutamine, in 100% D₂O, pH: 7.8
Experiment: SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Jul 2
Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux Journal of Applied Crystallography 58(2) (2025)
Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M
RgGuinier 4.3 nm
Dmax 11.7 nm