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26 hits found for DNA ligase A

...ASDJ52 – DNA repair protein XRCC1ΔN monomer/dimer

DNA repair protein XRCC1ΔN experimental SAS data
BILBOMD model
Sample: DNA repair protein XRCC1ΔN dimer, 76 kDa ...apiens protein
Buffer: ...aCl, 10% glycerol, 2 mM DTT, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2011 Jul 15
An atypical BRCT-BRCT interaction with the XRCC1 scaffold protein compacts human DNA Ligase IIIα within a flexible DNA repair complex. ...Acids Res (2020)
...ammel M, Rashid I, Sverzhinsky A, Pourfarjam Y, Tsai MS, Ellenberger T, Pascal JM, Kim IK, Tainer JA, Tomkinson AE
RgGuinier 5.4 nm
Dmax 19.5 nm
VolumePorod 170 nm3

...ASDJ62 – DNA repair protein XRCC1 monomer /dimer

DNA repair protein XRCC1 experimental SAS data
BILBOMD model
Sample: DNA repair protein XRCC1 , 69 kDa ...apiens protein
Buffer: ...aCl, 20 mM Tris-HCl, pH 7.5, 2% glycerol, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 19
An atypical BRCT-BRCT interaction with the XRCC1 scaffold protein compacts human DNA Ligase IIIα within a flexible DNA repair complex. ...Acids Res (2020)
...ammel M, Rashid I, Sverzhinsky A, Pourfarjam Y, Tsai MS, Ellenberger T, Pascal JM, Kim IK, Tainer JA, Tomkinson AE
RgGuinier 6.3 nm
Dmax 26.9 nm
VolumePorod 330 nm3

...ASDJ72 – DNA Ligase IIIα monomer/dimer

DNA ligase 3 (DNA ligase III alpha) experimental SAS data
BILBOMD model
Sample: DNA ligase 3 (DNA ligase III alpha) , ...apiens protein
Buffer: ...aCl, 10% glycerol, 2 mM DTT, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...ar 31
An atypical BRCT-BRCT interaction with the XRCC1 scaffold protein compacts human DNA Ligase IIIα within a flexible DNA repair complex. ...Acids Res (2020)
...ammel M, Rashid I, Sverzhinsky A, Pourfarjam Y, Tsai MS, Ellenberger T, Pascal JM, Kim IK, Tainer JA, Tomkinson AE
RgGuinier 6.1 nm
Dmax 21.0 nm
VolumePorod 240 nm3

...ASDJ82 – DNA repair protein XRCC1 - DNA Ligase IIIα complex

DNA repair protein XRCC1DNA ligase 3 (DNA ligase III alpha) experimental SAS data
BILBOMD model
Sample: DNA repair protein XRCC1 , 69 kDa ...apiens protein
DNA ligase 3 (DNA ligase III alpha) , ...apiens protein
Buffer: ...aCl, 5 mM MgCl₂,  0.2 mM PMSF, 1 mM benzamidine, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on ...Aug 18
An atypical BRCT-BRCT interaction with the XRCC1 scaffold protein compacts human DNA Ligase IIIα within a flexible DNA repair complex. ...Acids Res (2020)
...ammel M, Rashid I, Sverzhinsky A, Pourfarjam Y, Tsai MS, Ellenberger T, Pascal JM, Kim IK, Tainer JA, Tomkinson AE
RgGuinier 6.2 nm
Dmax 27.9 nm
VolumePorod 675 nm3

...ASDBR3 – ...A mutant): conjugated to Ubiquitin

E3 ubiquitin-protein ligase RNF8Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-C experimental SAS data
MES-FOXS model
Sample: ...ligase RNF8 dimer, 35 kDa ...apiens protein
...ating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa ...apiens protein
Polyubiquitin-C dimer, 17 kDa ...apiens protein
Buffer: ...aCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8
...Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...ail IH, Edwards RA, Hura GL, Xiao AT, Tainer JA, Hendzel MJ, Glover JN
RgGuinier 4.5 nm
Dmax 18.9 nm
VolumePorod 119 nm3

...ASDBS3 – ...A mutant) and Mms2: conjugated to Ubiquitin

E3 ubiquitin-protein ligase RNF8Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CUbiquitin-conjugating enzyme E2 variant 2 experimental SAS data
MES-FOXS model
Sample: ...ligase RNF8 dimer, 35 kDa ...apiens protein
...ating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa ...apiens protein
Polyubiquitin-C dimer, 17 kDa ...apiens protein
...ating enzyme E2 variant 2 dimer, 34 kDa ...apiens protein
Buffer: ...aCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8
...Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...ail IH, Edwards RA, Hura GL, Xiao AT, Tainer JA, Hendzel MJ, Glover JN
RgGuinier 5.4 nm
Dmax 23.8 nm
VolumePorod 214 nm3

...ASDBT3 – ...ant) complexed with Ubc13 (C87K, K92A mutant): conjugated to Ubiquitin

Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CE3 ubiquitin-protein ligase RNF8 mutant (L451D) experimental SAS data
MES-FOXS model
Sample: ...ating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa ...apiens protein
Polyubiquitin-C dimer, 17 kDa ...apiens protein
...ligase RNF8 mutant (L451D) dimer, 35 kDa ...apiens protein
Buffer: ...aCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8
...Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...ail IH, Edwards RA, Hura GL, Xiao AT, Tainer JA, Hendzel MJ, Glover JN
RgGuinier 4.3 nm
Dmax 18.9 nm
VolumePorod 111 nm3

...ASDBU3 – ...ant) complexed with Ubc13 (C87K, K92A mutant) and Mms2: conjugated to Ubiquitin

Ubiquitin-conjugating enzyme E2 N double mutant (C87K, K92A)Polyubiquitin-CUbiquitin-conjugating enzyme E2 variant 2E3 ubiquitin-protein ligase RNF8 mutant (L451D) experimental SAS data
MES-FOXS model
Sample: ...ating enzyme E2 N double mutant (C87K, K92A) dimer, 36 kDa ...apiens protein
Polyubiquitin-C dimer, 17 kDa ...apiens protein
...ating enzyme E2 variant 2 dimer, 34 kDa ...apiens protein
...ligase RNF8 mutant (L451D) dimer, 35 kDa ...apiens protein
Buffer: ...aCl 0.01 mM ZnSO4 1 mM DTT, pH: 6.8
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 8
...Ligase Stimulates Ubc13 E2 Conjugating Activity That Is Essential for DNA Double Strand Break Signaling and BRCA1 Tumor Suppressor Recruitment. J Biol Chem 291(18):9396-410 (2016)
...ail IH, Edwards RA, Hura GL, Xiao AT, Tainer JA, Hendzel MJ, Glover JN
RgGuinier 5.2 nm
Dmax 23.8 nm
VolumePorod 192 nm3

...ASDFG6 – ...able exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex

Beta sliding clampProbable exodeoxyribonuclease III protein XthADNA ligase A experimental SAS data
DAMMIF model
Sample: ...a sliding clamp dimer, 86 kDa ...acterium tuberculosis protein
...able exodeoxyribonuclease III protein XthA monomer, 31 kDa ...acterium tuberculosis protein
DNA ligase A monomer, 76 kDa ...acterium tuberculosis protein
Buffer: ...aCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at BM29, ESRF on ...ay 17
...ation of futile ligation during early steps of BER in M. tuberculosis is carried out by a β-clamp-XthA-LigA tri-component complex ...ational Journal of Biological Macromolecules (2022)
...a A, Afsar M, Khanam T, Kumar N, Ali F, Kumar S, Jahan F, Ramachandran R
RgGuinier 5.8 nm
Dmax 25.3 nm
VolumePorod 501 nm3

...ASDFH6 – ...able exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate

Probable exodeoxyribonuclease III protein XthADNA ligase ABeta sliding clampDNA ligase A nicked DNA substrate experimental SAS data
DAMMIF model
Sample: ...able exodeoxyribonuclease III protein XthA monomer, 31 kDa ...acterium tuberculosis protein
DNA ligase A monomer, 76 kDa ...acterium tuberculosis protein
...a sliding clamp dimer, 86 kDa ...acterium tuberculosis protein
DNA ligase A nicked DNA substrate dimer, 16 kDa DNA
Buffer: ...aCl , 2 mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at BM29, ESRF on ...ay 17
...ation of futile ligation during early steps of BER in M. tuberculosis is carried out by a β-clamp-XthA-LigA tri-component complex ...ational Journal of Biological Macromolecules (2022)
...a A, Afsar M, Khanam T, Kumar N, Ali F, Kumar S, Jahan F, Ramachandran R
RgGuinier 5.8 nm
Dmax 19.1 nm
VolumePorod 496 nm3

...ASDU57 – DNA repair protein XRCC4 in complex with BRCT domains of DNA ligase 4

DNA repair protein XRCC4DNA ligase 4 experimental SAS data
DNA repair protein XRCC4 DNA ligase 4 Kratky plot
Sample: DNA repair protein XRCC4 dimer, 77 kDa ...apiens protein
DNA ligase 4 monomer, 31 kDa ...apiens protein
Buffer: ...A, 1 mM DTT, pH: 6.5
Experiment: ...AXS data collected at SWING, SOLEIL on ...ar 13
...alent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro. ...at Struct Mol Biol (2024)
...A, Brenière M, Cisneros-Aguirre M, Pelupessy P, Wang Z, Carlier L, Bouvignies G, Cortes P, Aggarwal AK, Blackledge M, Gueroui Z, Belle V, Stark JM, Modesti M, Ferrage F
RgGuinier 5.8 nm
Dmax 23.0 nm
VolumePorod 248 nm3

...ASDJN2 – ...DNA phosphodiesterase 1 (TDP1)

Tyrosyl-DNA phosphodiesterase 1 experimental SAS data
BILBOMD model
Sample: ...DNA phosphodiesterase 1 monomer, 71 kDa ...apiens protein
Buffer: ...aCl, 20 mM Tris-HCl, pH 7.5, 2% glycerol,, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 26
...action of DNA repair protein tyrosyl DNA phosphodiesterase 1 and the DNA ligase III catalytic domain is regulated by phosphorylation of its flexible N-terminus. J Biol Chem :100921 (2021)
...ashid I, Hammel M, Sverzhinsky A, Tsai MS, Pascal JM, Tainer JA, Tomkinson AE
RgGuinier 3.2 nm
Dmax 11.7 nm
VolumePorod 143 nm3

...ASDEU5 – Adenylation domain of DNA ligase A

DNA ligase A experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 38 kDa ...acterium tuberculosis protein
Buffer: ...aCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at Anton Paar SAXSpace, ...al Drug Research Institute on 2018 Sep 27
...alt bridges at the subdomain interfaces of the adenylation domain and active-site residues of Mycobacterium tuberculosis NAD + -dependent DNA ligase A (MtbLigA) are importa... Acta Crystallographica Section D Structural Biology 77(6) (2021)
Afsar M, Shukla A, Kumar N, Ramachandran R
RgGuinier 2.4 nm
Dmax 8.8 nm
VolumePorod 69 nm3

...ASDDD8 – ...ass II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III

Probable exodeoxyribonuclease III protein XthA experimental SAS data
DAMMIF model
Sample: ...able exodeoxyribonuclease III protein XthA monomer, 32 kDa ...acterium tuberculosis protein
Buffer: ...aCl 5mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at BM29, ESRF on ...ay 12
...ass II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. ...Acids Res (2020)
...anam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 2.4 nm
Dmax 7.3 nm
VolumePorod 56 nm3

...ASDJQ2 – ...ated tyrosyl-DNA phosphodiesterase 1 (TDP1-Ppi)

Tyrosyl-DNA phosphodiesterase 1 (dephosphorylated) experimental SAS data
BILBOMD model
Sample: ...DNA phosphodiesterase 1 (dephosphorylated) monomer, 71 kDa ...apiens protein
Buffer: ...aCl, 20 mM Tris-HCl, pH 7.5, 2% glycerol, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jun 26
...action of DNA repair protein tyrosyl DNA phosphodiesterase 1 and the DNA ligase III catalytic domain is regulated by phosphorylation of its flexible N-terminus. J Biol Chem :100921 (2021)
...ashid I, Hammel M, Sverzhinsky A, Tsai MS, Pascal JM, Tainer JA, Tomkinson AE
RgGuinier 3.2 nm
Dmax 16.5 nm
VolumePorod 141 nm3

...ASDEC6 – Adenylation Domain of DNA ligase A with NAD+

DNA ligase A experimental SAS data
DAMMIF model
Sample: DNA ligase A monomer, 38 kDa ...acterium tuberculosis protein
Buffer: ...aCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at Anton Paar SAXSpace, ...al Drug Research Institute on 2018 Oct 6
...alt bridges at the subdomain interfaces of the adenylation domain and active-site residues of Mycobacterium tuberculosis NAD + -dependent DNA ligase A (MtbLigA) are importa... Acta Crystallographica Section D Structural Biology 77(6) (2021)
Afsar M, Shukla A, Kumar N, Ramachandran R
RgGuinier 2.4 nm
Dmax 6.2 nm
VolumePorod 94 nm3

...ASDDN9 – ...acterium tuberculosis DNA Ligase A

DNA ligase A experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa ...acterium tuberculosis protein
Buffer: ...aCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at BM29, ESRF on ...ay 13
...ass II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. ...Acids Res (2020)
...anam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 5.2 nm
Dmax 16.7 nm
VolumePorod 870 nm3

...ASDJP2 – ...ated tyrosyl-DNA phosphodiesterase 1 (TDP1 149-608)

Tyrosyl-DNA phosphodiesterase 1 (149-608) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...DNA phosphodiesterase 1 (149-608) monomer, 53 kDa ...apiens protein
Buffer: ...aCl, 20 mM Tris-HCl, pH 7.5, 2% glycerol,, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 18
...action of DNA repair protein tyrosyl DNA phosphodiesterase 1 and the DNA ligase III catalytic domain is regulated by phosphorylation of its flexible N-terminus. J Biol Chem :100921 (2021)
...ashid I, Hammel M, Sverzhinsky A, Tsai MS, Pascal JM, Tainer JA, Tomkinson AE
RgGuinier 2.3 nm
Dmax 7.0 nm
VolumePorod 80 nm3

...ASDG65 – Adenylation domain of DNA ligase A (LigA)

DNA ligase A experimental SAS data
GASBOR model
Sample: DNA ligase A monomer, 37 kDa ...acterium tuberculosis protein
Buffer: ...aCl 2mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at Anton Paar SAXSpace, ...al Drug Research Institute on ...ay 21
...alt bridges at the subdomain interfaces of the adenylation domain and active-site residues of Mycobacterium tuberculosis NAD + -dependent DNA ligase A (MtbLigA) are importa... Acta Crystallographica Section D Structural Biology 77(6) (2021)
Afsar M, Shukla A, Kumar N, Ramachandran R
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 68 nm3

...ASDDV9 – ...acterium tuberculosis DNA LigaseA with Nicked DNA

DNA ligase ANicked DNA experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa ...acterium tuberculosis protein
...DNA dimer, 16 kDa DNA
Buffer: ...aCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at BM29, ESRF on ...ay 13
...ass II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. ...Acids Res (2020)
...anam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 4.4 nm
Dmax 14.8 nm
VolumePorod 262 nm3

...ASDJS2 – ...ated DNA ligase 3 bound to tyrosyl-DNA phosphodiesterase 1 (LigIIIα(170-755)/TDP1 complex)

Tyrosyl-DNA phosphodiesterase 1Isoform 3 of DNA ligase 3 (DNA ligase III alpha) experimental SAS data
BILBOMD model
Sample: ...DNA phosphodiesterase 1 monomer, 71 kDa ...apiens protein
...DNA ligase 3 (DNA ligase III alpha) monomer, 68 kDa ...apiens protein
Buffer: ...aCl, 40 mM HEPES, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Jul 5
...action of DNA repair protein tyrosyl DNA phosphodiesterase 1 and the DNA ligase III catalytic domain is regulated by phosphorylation of its flexible N-terminus. J Biol Chem :100921 (2021)
...ashid I, Hammel M, Sverzhinsky A, Tsai MS, Pascal JM, Tainer JA, Tomkinson AE
RgGuinier 4.5 nm
Dmax 18.0 nm
VolumePorod 211 nm3

...ASDEW3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA)

DNA ligase AProbable exodeoxyribonuclease III protein XthA experimental SAS data
DAMMIF model
Sample: DNA ligase A monomer, 76 kDa ...acterium tuberculosis protein
...able exodeoxyribonuclease III protein XthA monomer, 33 kDa ...acterium tuberculosis protein
Buffer: ...aCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at BM29, ESRF on ...ay 13
...ass II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. ...Acids Res (2020)
...anam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 6.2 nm
Dmax 23.9 nm
VolumePorod 662 nm3

...ASDJR2 – DNA ligase 3 bound to tyrosyl-DNA phosphodiesterase 1 (LigIIIα/TDP1 complex)

DNA ligase 3 (DNA ligase III alpha)Tyrosyl-DNA phosphodiesterase 1 experimental SAS data
BILBOMD model
Sample: DNA ligase 3 (DNA ligase III alpha) , ...apiens protein
...DNA phosphodiesterase 1 monomer, 71 kDa ...apiens protein
Buffer: ...aCl, 40 mM HEPES, pH: 7.5
Experiment: ...AXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Sep 10
...action of DNA repair protein tyrosyl DNA phosphodiesterase 1 and the DNA ligase III catalytic domain is regulated by phosphorylation of its flexible N-terminus. J Biol Chem :100921 (2021)
...ashid I, Hammel M, Sverzhinsky A, Tsai MS, Pascal JM, Tainer JA, Tomkinson AE
RgGuinier 6.5 nm
Dmax 25.5 nm
VolumePorod 790 nm3

...ASDEX3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) in presence of nicked ds DNA

DNA ligase AProbable exodeoxyribonuclease III protein XthANicked DNA experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 76 kDa ...acterium tuberculosis protein
...able exodeoxyribonuclease III protein XthA monomer, 33 kDa ...acterium tuberculosis protein
...DNA monomer, 8 kDa DNA
Buffer: ...aCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at BM29, ESRF on ...ay 13
...ass II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. ...Acids Res (2020)
...anam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 4.5 nm
Dmax 17.1 nm
VolumePorod 117 nm3

...ASDDQ8 – ...ass II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III (XthA) bound to the BRCT domain from Mycobacterium tuberculosis DNA ligase

Probable exodeoxyribonuclease III protein XthAM. tb. LigA BRCT domain experimental SAS data
DAMFILT model
Sample: ...able exodeoxyribonuclease III protein XthA monomer, 33 kDa ...acterium tuberculosis protein
...A BRCT domain monomer, 15 kDa ...acterium tuberculosis protein
Buffer: ...aCl 5mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at BM29, ESRF on ...ar 9
...ass II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. ...Acids Res (2020)
...anam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 3.7 nm
Dmax 18.5 nm
VolumePorod 112 nm3

...ASDD88 – ...ain from Mycobacterium tuberculosis DNA ligase

M.tb. LigA BRCT domain (DNA ligase A) experimental SAS data
DAMMIN model
Sample: ...A BRCT domain (DNA ligase A) monomer, 13 kDa ...acterium tuberculosis protein
Buffer: ...aCl 5mM β-mercaptoethanol, pH: 8
Experiment: ...AXS data collected at Anton Paar SAXSpace, ...al Drug Research Institute on 2018 Jun 2
...ass II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision repair. ...Acids Res (2020)
...anam T, Afsar M, Shukla A, Alam F, Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R
RgGuinier 1.6 nm
Dmax 3.7 nm
VolumePorod 23 nm3