Small Angle Scattering Biological Data Bank SASBDB


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29 hits found for DNA protection during starvation protein

SASDKD4 – ...DNA protection during starvation protein (Dps)/DNA co-crystallization

DNA protection during starvation protein experimental SAS data

DNA protection during starvation protein Kratky plot

Sample:	DNA protection during starvation protein dodecamer, 224 kDa Escherichia coli (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 27

...DNA co-crystallization in stressed cells: an in vitro structural study by small-angle X-ray scattering and cryo-electron tomography. FEBS Lett 593(12):1360-1371 (2019)
Dadinova LA, Chesnokov YM, Kamyshinsky RA, Orlov IA, Petoukhov MV, Mozhaev AA, Soshinskaya EY, Lazarev VN, Manuvera VA, Orekhov AS, Vasiliev AL, Shtykova EV

SASDKE4 – ...DNA protection during starvation protein (Dps)-DNA сo-сrystals

Sample:	DNA protection during starvation protein dodecamer, 224 kDa Escherichia coli (strain … protein
Buffer:	10 mM Tris-HCl, 100 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Oct 28

...DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering. Biomolecules 10(1) (2019)
Kamyshinsky R, Chesnokov Y, Dadinova L, Mozhaev A, Orlov I, Petoukhov M, Orekhov A, Shtykova E, Vasiliev A

SASDKF4 – DNA binding protein of starvation (DPS)

Sample:	DNA protection during starvation protein dodecamer, 225 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 26

...DNA-Dps Complexes. J Mol Biol :166930 (2021)
Dubrovin EV, Dadinova LA, Petoukhov MV, Yu Soshinskaya E, Mozhaev AA, Klinov DV, Schäffer TE, Shtykova EV, Batishchev OV

R_g^Guinier	3.9	nm
D_max	14.0	nm
Volume^Porod	314	nm³

SASDQT4 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS (Ferritin superfamily) experimental SAS data

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

Controlled modulation of the dynamics of the Deinococcus grandis Dps N-terminal tails by divalent metals. Protein Sci :e4567 (2023)
Guerra JPL, Blanchet CE, Vieira BJC, Waerenborgh JC, Jones NC, Hoffmann SV, Pereira AS, Tavares P

R_g^Guinier	4.5	nm
D_max	20.0	nm
Volume^Porod	454	nm³

SASDQU4 – DNA-binding protein from starved cells: DgrDpsWT + 6 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	18.4	nm
Volume^Porod	440	nm³

SASDQV4 – DNA-binding protein from starved cells: DgrDpsWT + 12 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	15.5	nm
Volume^Porod	464	nm³

SASDQW4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	14.1	nm
Volume^Porod	439	nm³

SASDQX4 – DNA-binding protein from starved cells: DgrDpsWT + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	14.4	nm
Volume^Porod	427	nm³

SASDKY4 – DNA binding protein of starvation (no EDTA)

Sample:	DNA protection during starvation protein dodecamer, 225 kDa Escherichia coli protein
Buffer:	20мМ Tris-HCl, 100 мМ NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 19

...DNA-Dps Complexes. J Mol Biol :166930 (2021)
Dubrovin EV, Dadinova LA, Petoukhov MV, Yu Soshinskaya E, Mozhaev AA, Klinov DV, Schäffer TE, Shtykova EV, Batishchev OV

R_g^Guinier	3.8	nm
D_max	11.9	nm
Volume^Porod	277	nm³

SASDQY4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl, 5 mM EDTA

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS, 230 mM NaCl, 5 mM EDTA, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.5	nm
Volume^Porod	453	nm³

SASDQZ4 – DNA-binding protein from starved cells: DgrDpsWT + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.2	nm
D_max	13.9	nm
Volume^Porod	458	nm³

SASDQ25 – DNA-binding protein from starved cells: DgrDpsWT + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	19.3	nm
Volume^Porod	438	nm³

SASDQ35 – DNA-binding protein from starved cells: DgrDps-D43A mutant in 50 mM MOPS pH 7.0, 230 mM NaCl

DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation experimental SAS data

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.8	nm
Volume^Porod	451	nm³

SASDQ45 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	19.1	nm
Volume^Porod	412	nm³

SASDQ55 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 48 Zn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.4	nm
D_max	20.7	nm
Volume^Porod	409	nm³

SASDQ65 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Co2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	20.6	nm
Volume^Porod	404	nm³

SASDQ75 – DNA-binding protein from starved cells: DgrDps-D43A mutant + 24 Mn2+ in 50 mM MOPS pH 7.0, 230 mM NaCl

Sample:	DNA protection during starvation, DPS-D43A (Ferritin superfamily) dodecamer D43A mutation dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Nov 8

R_g^Guinier	4.5	nm
D_max	19.5	nm
Volume^Porod	431	nm³

SASDPX7 – DNA protection during starvation protein (Dps) loaded with FeSO4⋅7H2O in the amount corresponding to 50 iron atoms per dodecamer. Anomalous SAXS spanning the X-ray Fe-absorption edge 7.100-7.133 keV.

Sample:	DNA protection during starvation protein dodecamer, 224 kDa Escherichia coli (strain … protein
Buffer:	50 mM NaCl, 0.5 mM EDTA, 50 mM Tris-HCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Oct 5

...Protein Dps Moscow University Physics Bulletin 77(6):858-867 (2023)
Gordienko A, Mozhaev A, Gibizova V, Dadinova L

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	32	nm³

SASDNY7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 50 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength International Journal of Molecular Sciences 23(9):4871 (2022)
Guerra J, Blanchet C, Vieira B, Almeida A, Waerenborgh J, Jones N, Hoffmann S, Tavares P, Pereira A

R_g^Guinier	4.4	nm
D_max	16.1	nm
Volume^Porod	409	nm³

SASDPY7 – DNA protection during starvation protein (Dps) loaded with FeSO4⋅7H2O in the amount corresponding to 500 iron atoms per dodecamer. Anomalous SAXS spanning the X-ray Fe-absorption edge 7.100-7.133 keV.

Sample:	DNA protection during starvation protein dodecamer, 224 kDa Escherichia coli (strain … protein
Buffer:	50 mM NaCl, 0.5 mM EDTA, 50 mM Tris-HCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Oct 5

...Protein Dps Moscow University Physics Bulletin 77(6):858-867 (2023)
Gordienko A, Mozhaev A, Gibizova V, Dadinova L

R_g^Guinier	7.4	nm
D_max	12.0	nm

SASDNZ7 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 80 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 80 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.5	nm
D_max	17.1	nm
Volume^Porod	475	nm³

SASDPZ7 – DNA protection during starvation protein (Dps) loaded with FeSO4⋅7H2O in the amount corresponding to 2000 iron atoms per dodecamer. Anomalous SAXS spanning the X-ray Fe-absorption edge 7.100-7.133 keV...

Sample:	DNA protection during starvation protein dodecamer, 224 kDa Escherichia coli (strain … protein
Buffer:	50 mM NaCl, 0.5 mM EDTA, 50 mM Tris-HCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Oct 5

...Protein Dps Moscow University Physics Bulletin 77(6):858-867 (2023)
Gordienko A, Mozhaev A, Gibizova V, Dadinova L

R_g^Guinier	7.1	nm
D_max	14.0	nm

SASDN28 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 230 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.5	nm
D_max	20.6	nm
Volume^Porod	438	nm³

SASDN38 – DNA-binding protein from starved cells: DgrDpsWT in 50 mM MOPS, 480 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS (Ferritin superfamily) dodecamer, 270 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 480 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	4.8	nm
D_max	20.5	nm
Volume^Porod	430	nm³

SASDN48 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 50 mM NaCl pH 7.0

DNA protection during starvation, DPS-∆N (Ferritin superfamily) experimental SAS data

Sample:	DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 50 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	3.8	nm
D_max	9.3	nm
Volume^Porod	290	nm³

SASDN58 – DNA-binding protein from starved cells (tailless): DgrDps∆N in 50 mM MOPS, 230 mM NaCl pH 7.0

Sample:	DNA protection during starvation, DPS-∆N (Ferritin superfamily) dodecamer, 218 kDa Deinococcus grandis protein
Buffer:	50 mM MOPS-NaOH, 230 mM NaCl, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Oct 22

R_g^Guinier	3.8	nm
D_max	8.8	nm
Volume^Porod	291	nm³

SASDBG7 – ...DNA binding protein under starvation conditions (SEC-SAXS)

DNA protection during starvation protein 1 experimental SAS data

Sample:	DNA protection during starvation protein 1 dodecamer, 276 kDa Deinococcus radiodurans R1 protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions. J Mol Biol 429(5):667-687 (2017)
Santos SP, Cuypers MG, Round A, Finet S, Narayanan T, Mitchell EP, Romão CV

R_g^Guinier	4.2	nm
D_max	12.8	nm
Volume^Porod	437	nm³

SASDBH7 – ...DNA binding protein under starvation conditions (SEC-SAXS)

N-terminal truncated DNA protection during starvation protein 1 experimental SAS data

Sample:	...DNA protection during starvation protein 1 dodecamer, 216 kDa Deinococcus radiodurans R1 protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

R_g^Guinier	3.9	nm
D_max	10.0	nm
Volume^Porod	291	nm³

SASDBF7 – ...DNA binding protein under starvation conditions (SEC-SAXS)

N-terminal truncated DNA protection during starvation protein 2 experimental SAS data

Sample:	...DNA protection during starvation protein 2 dodecamer, 279 kDa Deinococcus radiodurans R1 protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

R_g^Guinier	4.2	nm
D_max	12.7	nm
Volume^Porod	445	nm³

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