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14 hits found for DNA-directed RNA polymerase

SASDJT2 – DNA-directed RNA polymerase (RPO41)

DNA-directed RNA polymerase experimental SAS data
DNA-directed RNA polymerase Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Sep 25
...RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 4.4 nm
Dmax 12.5 nm
VolumePorod 295 nm3

SASDJU2 – DNA-directed RNA polymerase (RPO41) in complex with rRNA adenine N(6)-methyltransferase (PfKsgA1)

DNA-directed RNA polymeraserRNA adenine N (6) methyl transferase experimental SAS data
DNA-directed RNA polymerase rRNA adenine N (6) methyl transferase Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
...RNA adenine N (6) methyl transferase monomer, 39 kDa Plasmodium falciparum protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2020 Jan 10
...RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 5.5 nm
Dmax 15.0 nm
VolumePorod 393 nm3

SASDJV2 – DNA-directed RNA polymerase (RPO41) in complex with mitochondrial transcription factor 1 (MTF1)

DNA-directed RNA polymeraseMitochondrial transcription factor 1 experimental SAS data
DNA-directed RNA polymerase Mitochondrial transcription factor 1 Kratky plot
Sample: DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Mitochondrial transcription factor 1 monomer, 40 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Dec 7
...RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S
RgGuinier 4.5 nm
Dmax 14.4 nm
VolumePorod 292 nm3

SASDCZ2 – ...DNA-directed RNA polymerase II subunit RPB1 (Rtt103 1-246-phospho-mimetic complex)

Regulator of Ty1 transposition protein 103DNA-directed RNA polymerase II subunit RPB1 experimental SAS data
CORAL model
Sample: Regulator of Ty1 transposition protein 103 dimer, 57 kDa Saccharomyces cerevisiae protein
DNA-directed RNA polymerase II subunit RPB1 monomer, 13 kDa Saccharomyces cerevisiae protein
Buffer: 25 mM KH2PO4, 300 mM KCl, 10 mM BME, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 Apr 12
...RNAPII CTDsome with Rtt103. Proc Natl Acad Sci U S A 114(42):11133-11138 (2017)
Jasnovidova O, Klumpler T, Kubicek K, Kalynych S, Plevka P, Stefl R
RgGuinier 5.3 nm
Dmax 21.0 nm
VolumePorod 561 nm3

SASDJK4 – RNA polymerase core complex

DNA-directed RNA polymerase subunit alphaDNA-directed RNA polymerase subunit betaDNA-directed RNA polymerase subunit beta'DNA-directed RNA polymerase subunit omega experimental SAS data
DAMFILT model
Sample: DNA-directed RNA polymerase subunit alpha dimer, 78 kDa Mycobacterium tuberculosis protein
DNA-directed RNA polymerase subunit beta monomer, 129 kDa Mycobacterium tuberculosis protein
DNA-directed RNA polymerase subunit beta' monomer, 147 kDa Mycobacterium tuberculosis protein
DNA-directed RNA polymerase subunit omega monomer, 12 kDa Mycobacterium tuberculosis protein
Buffer: 10 mM Tris-Cl, 100 mM NaCl, 10 mM MgCl2, 0.1 mM EDTA, 5% Glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 8
...RNA polymerase core and UvrD complex: a key component in transcription coupled DNA repair
Ravishankar Ramachandran
RgGuinier 5.1 nm
Dmax 19.3 nm
VolumePorod 262 nm3

SASDJL4 – RNA polymerase core subunit in complex with ATP-dependent DNA helicase UvrD1

DNA-directed RNA polymerase subunit alphaDNA-directed RNA polymerase subunit betaDNA-directed RNA polymerase subunit beta'DNA-directed RNA polymerase subunit omegaATP-dependent DNA helicase UvrD1 experimental SAS data
DAMMIF model
Sample: DNA-directed RNA polymerase subunit alpha dimer, 75 kDa Mycobacterium tuberculosis protein
DNA-directed RNA polymerase subunit beta monomer, 129 kDa Mycobacterium tuberculosis protein
DNA-directed RNA polymerase subunit beta' monomer, 147 kDa Mycobacterium tuberculosis protein
DNA-directed RNA polymerase subunit omega monomer, 12 kDa Mycobacterium tuberculosis protein
ATP-dependent DNA helicase UvrD1 monomer, 85 kDa Mycobacterium tuberculosis protein
Buffer: 10 mM Tris-Cl, 100 mM NaCl, 10 mM MgCl2, 0.1 mM EDTA, 5% Glycerol, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 11
...RNA polymerase core and UvrD complex: a key component in transcription coupled DNA repair
Ravishankar Ramachandran
RgGuinier 7.5 nm
Dmax 22.2 nm
VolumePorod 898 nm3

SASDF58 – ...RNA polymerase, RNAP (B. subtilis), 10mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
...RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 38 nm3

SASDF68 – ...RNA polymerase, RNAP (B. subtilis), 200 mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
...RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 3.9 nm
Dmax 20.0 nm
VolumePorod 56 nm3

SASDF78 – ...RNA polymerase, RNAP (B. subtilis), 400 mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
...RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 4.2 nm
Dmax 20.0 nm
VolumePorod 63 nm3

SASDF88 – ...RNA polymerase, RNAP (B. subtilis), 800 mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
...RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 4.5 nm
Dmax 21.0 nm
VolumePorod 77 nm3

SASDF98 – ...RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
...RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 4.3 nm
Dmax 19.5 nm
VolumePorod 58 nm3

SASDFA8 – ...RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
...RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 4.6 nm
Dmax 22.0 nm
VolumePorod 76 nm3

SASDFB8 – ...RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
...RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 4.5 nm
Dmax 24.0 nm
VolumePorod 78 nm3

SASDFC8 – ...RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 800mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
...RNA Polymerase. J Am Chem Soc (2019)
Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zi...
RgGuinier 4.5 nm
Dmax 21.0 nm
VolumePorod 74 nm3