Small Angle Scattering Biological Data Bank SASBDB


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6 hits found for Histone H2A type 1

SASDJ96 – Trinucleosomes from Xenopus laevis (African clawed frog)

Histone H3Histone H4Histone H2A type 1Histone H2BNon-linker Ended Trinucleosome DNA experimental SAS data

Histone H3 Histone H4 Histone H2A type 1 Histone H2B Non-linker Ended Trinucleosome DNA Kratky plot

Sample:	Histone H3 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B monomer, 14 kDa Xenopus laevis protein Non-linker Ended Trinucleosome DNA monomer, 172 kDa DNA
Buffer:	...150 mM NaCl 1 mM EDTA 1 mM DTT 50% w/v sucrose, pH: 7.5
Experiment:	SAXS data collected at ...1, Cornell High Energy Synchrotron Source (CHESS) on ...16 Mar 11

Solution structure(s) of trinucleosomes from contrast variation SAXS Nucleic Acids Research (2021)
Mauney A, Muthurajan U, Luger K, Pollack L

R_g^Guinier	12.9	nm
D_max	41.8	nm

SASDCT6 – 12N12 nucleosome in 60% sucrose with ADP-BeF3

169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)Histone H2A type 1Histone H2B 1.1Histone H3.2Histone H4 experimental SAS data

12N12 nucleosome in 60% sucrose with ADP-BeF3 Rg histogram

Sample:	169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein Histone H3.2 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer:	10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2), pH: 7.8
Experiment:	SAXS data collected at ...1, Cornell High Energy Synchrotron Source (CHESS) on ...15 Oct 24

...1 chromatin remodeler stimulates DNA unwrapping from the nucleosome. Nucleic Acids Res 46(10):4978-4990 (2018)
Tokuda JM, Ren R, Levendosky RF, Tay RJ, Yan M, Pollack L, Bowman GD

R_g^Guinier	4.8	nm
D_max	14.0	nm

SASDCU6 – ...1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose without any nucleotides added (Apo)

Chromodomain-helicase-DNA-binding protein 1169 bp DNA (145 bp Widom 601, flanked by 12bp DNA)Histone H2A type 1Histone H2B 1.1Histone H3.2Histone H4 experimental SAS data

Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose without any nucleotides added (Apo) Rg histogram

Sample:	...1 dimer, 266 kDa Saccharomyces cerevisiae protein 169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein Histone H3.2 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer:	10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, pH: 7.8
Experiment:	SAXS data collected at ...1, Cornell High Energy Synchrotron Source (CHESS) on ...15 Oct 24

...1 chromatin remodeler stimulates DNA unwrapping from the nucleosome. Nucleic Acids Res 46(10):4978-4990 (2018)
Tokuda JM, Ren R, Levendosky RF, Tay RJ, Yan M, Pollack L, Bowman GD

R_g^Guinier	5.2	nm
D_max	12.8	nm

SASDCV6 – ...1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with ADP-BeF3

Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with ADP-BeF3 Rg histogram

Sample:	...1 dimer, 266 kDa Saccharomyces cerevisiae protein 169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein Histone H3.2 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer:	10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, ADP-BeF3 (0.5 mM ADP, 4 mM NaF, 0.6 mM BeCl2), pH: 7.8
Experiment:	SAXS data collected at ...1, Cornell High Energy Synchrotron Source (CHESS) on ...15 Oct 24

...1 chromatin remodeler stimulates DNA unwrapping from the nucleosome. Nucleic Acids Res 46(10):4978-4990 (2018)
Tokuda JM, Ren R, Levendosky RF, Tay RJ, Yan M, Pollack L, Bowman GD

R_g^Guinier	5.3	nm
D_max	16.5	nm

SASDCW6 – ...1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with AMP-PNP

Chd1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with AMP-PNP Rg histogram

Sample:	...1 dimer, 266 kDa Saccharomyces cerevisiae protein 169 bp DNA (145 bp Widom 601, flanked by 12bp DNA) monomer, 52 kDa DNA Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein Histone H2B 1.1 monomer, 14 kDa Xenopus laevis protein Histone H3.2 monomer, 15 kDa Xenopus laevis protein Histone H4 monomer, 11 kDa Xenopus laevis protein
Buffer:	10 mM Tris, 100 mM NaCl, 2 mM MgCl2, 0.1 mM EDTA, 1 mM DTT, 60% (w/v) sucrose, 0.5 mM AMP-PNP, pH: 7.8
Experiment:	SAXS data collected at ...1, Cornell High Energy Synchrotron Source (CHESS) on ...15 Oct 24

...1 chromatin remodeler stimulates DNA unwrapping from the nucleosome. Nucleic Acids Res 46(10):4978-4990 (2018)
Tokuda JM, Ren R, Levendosky RF, Tay RJ, Yan M, Pollack L, Bowman GD

R_g^Guinier	5.6	nm
D_max	16.7	nm

SASDNR7 – ...1-136) -H2A/H2B histone oligomer complex

Peptidyl-prolyl cis-trans isomerase FKBP43Histone H2A type 1-B/EHistone H2B type 1-C/E/F/G/I experimental SAS data

Sample:	Peptidyl-prolyl cis-trans isomerase FKBP43 pentamer, 81 kDa Arabidopsis thaliana protein Histone H2A type 1-B/E monomer, 14 kDa Homo sapiens protein Histone H2B type 1-C/E/F/G/I monomer, 14 kDa Homo sapiens protein
Buffer:	...1 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on ...1 Apr 28

...histone interaction. Biochim Biophys Acta Gene Regul Mech 1865(7):194872 (2022)
Singh AK, Saharan K, Baral S, Vasudevan D

R_g^Guinier	4.1	nm
D_max	13.9	nm
Volume^Porod	194	nm³

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SASDJ96 – Trinucleosomes from Xenopus laevis (African clawed frog)

SASDCT6 – 12N12 nucleosome in 60% sucrose with ADP-BeF3

SASDCU6 – ...1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose without any nucleotides added (Apo)

SASDCV6 – ...1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with ADP-BeF3

SASDCW6 – ...1-12N12, chromatin remodeler--nucleosome complex, in 60% sucrose with AMP-PNP

SASDNR7 – ...1-136) -H2A/H2B histone oligomer complex