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10 hits found for Importin subunit alpha-1

SASDER2 – ...Importin Beta Binding domain (IBB) from importin subunit alpha-1 without denaturant

Importin subunit alpha-1 experimental SAS data
Unlabeled Importin Beta Binding domain (IBB) from importin subunit alpha-1 without denaturant Rg histogram
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 30 nm3

SASDES2 – ...Importin Beta Binding Domain (IBB-Alexa488/Alexa594) from importin subunit alpha-1 without denaturant

Importin subunit alpha-1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Importin subunit alpha-1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 9
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 2.9 nm
Dmax 13.7 nm
VolumePorod 29 nm3

SASDET2 – ...Importin Beta Binding Domain (IBB) from importin subunit alpha-1 with denaturant

Importin subunit alpha-1 experimental SAS data
Unlabeled Importin Beta Binding Domain (IBB) from importin subunit alpha-1 with denaturant Rg histogram
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 15.3 nm
VolumePorod 34 nm3

SASDEU2 – ...Importin Beta Binding Domain from importin subunit alpha-1 (IBB-Alexa488/Alexa594) with denaturant

Importin subunit alpha-1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Importin subunit alpha-1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 13.3 nm
VolumePorod 29 nm3

SASDUL9 – ...Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 11.6 nm
VolumePorod 23 nm3

SASDUM9 – ...Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 0.5 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 0.5 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.6 nm
Dmax 13.8 nm
VolumePorod 18 nm3

SASDUQ9 – ...Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.8 nm
VolumePorod 22 nm3

SASDUP9 – ...Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 2 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 2 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jul 1
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.0 nm
Dmax 14.4 nm
VolumePorod 31 nm3

SASDUN9 – ...Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea

Importin subunit alpha-1 experimental SAS data
Importin subunit alpha-1 Kratky plot
Sample: Importin subunit alpha-1 monomer, 9 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Jun 29
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 2.7 nm
Dmax 11.9 nm
VolumePorod 23 nm3

SASDUR9 – ...Importin Beta Binding domain (IBB) from importin subunit alpha-1 in 6 M Urea

importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK experimental SAS data
importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK Kratky plot
Sample: importin-beta-binding domain of importin subunit alpha-1 labelled with unnatural amino acid diBrK monomer, 8 kDa Homo sapiens protein
Buffer: phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 19
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins. Proc Natl Acad Sci U S A 121(50):e2415220121 (2024)
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
RgGuinier 3.0 nm
Dmax 12.3 nm
VolumePorod 28 nm3