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16 hits found for Replicase polyprotein 1a

SASDP23 – ...polyprotein monomer

Replicase polyprotein 1a experimental SAS data
DAMMIF model
Sample: Replicase polyprotein 1a monomer, 60 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
...polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 3.5 nm
Dmax 15.6 nm
VolumePorod 102 nm3

SASDP33 – ...polyprotein dimer

Replicase polyprotein 1a experimental SAS data
Replicase polyprotein 1a Kratky plot
Sample: Replicase polyprotein 1a dimer, 119 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
...polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 4.6 nm
Dmax 19.1 nm
VolumePorod 203 nm3

SASDKJ4 – SARS-CoV-2 nsp7 and nsp8

Replicase polyprotein 1a - nsp7Replicase polyprotein 1a - nsp8 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a - nsp7 , 18 kDa Severe acute respiratory … protein
Replicase polyprotein 1a - nsp8 , 44 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 19
Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes Science Advances 7(10):eabf1004 (2021)
Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 92 nm3

SASDJG5 – SARS-CoV-2 Main Protease

3C-like proteinase from SARS-CoV-2 replicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...replicase polyprotein 1a dimer, 68 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 1 mM DTT, 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2020 Jun 1
Crystallographic structure of wild-type SARS-CoV-2 main protease acyl-enzyme intermediate with physiological C-terminal autoprocessing site. Nat Commun 11(1):5877 (2020)
Lee J, Worrall LJ, Vuckovic M, Rosell FI, Gentile F, Ton AT, Caveney NA, Ban F, Cherkasov A, Paetzel M, Strynadka NCJ
RgGuinier 2.7 nm
Dmax 8.8 nm
VolumePorod 93 nm3

SASDJH5 – SARS-CoV-2 Main Protease, P9T mutant

3C-like proteinase from SARS-CoV-2 replicase polyprotein 1a, PT9 mutant experimental SAS data
SREFLEX model
Sample: ...replicase polyprotein 1a, PT9 mutant monomer, 34 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 1 mM DTT, 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2020 Jun 1
Crystallographic structure of wild-type SARS-CoV-2 main protease acyl-enzyme intermediate with physiological C-terminal autoprocessing site. Nat Commun 11(1):5877 (2020)
Lee J, Worrall LJ, Vuckovic M, Rosell FI, Gentile F, Ton AT, Caveney NA, Ban F, Cherkasov A, Paetzel M, Strynadka NCJ
RgGuinier 2.4 nm
Dmax 7.2 nm
VolumePorod 54 nm3

SASDP56 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 4.1 nm

SASDP66 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 500 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 98 nm3

SASDP76 – SARS-CoV-2 non-structural protein 8 (nsp8, dimer) in 1000 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 100 nm3

SASDP86 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 6.3 nm
Dmax 24.0 nm

SASDP96 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 500 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 5.2 nm
Dmax 18.0 nm

SASDPA6 – SARS-CoV-2 non-structural protein 8 (nsp8, tetramer) in 1000 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data
Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot
Sample: Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment: SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19
Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J
RgGuinier 4.8 nm
Dmax 16.0 nm

SASDKU6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14 complex (nsp10/nsp14 )

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 2.9 nm
Dmax 12.2 nm
VolumePorod 73 nm3

SASDKV6 – SARS-CoV-2 non-structural protein 10/non-structural protein 16 complex (nsp10/nsp16)

Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 2.0 nm
Dmax 8.0 nm
VolumePorod 28 nm3

SASDKW6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14/non-structural protein 16 triplex (nsp10/nsp14/nsp16)

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 4.7 nm
Dmax 11.4 nm
VolumePorod 113 nm3

SASDMJ9 – ...polyprotein

Replicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R
RgGuinier 2.1 nm

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer: 10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2
Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R
RgGuinier 3.0 nm