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11 hits found for Response regulator

SASDKA6 – ...regulator A (FrrA)

TetR/AcrR family transcriptional regulator experimental SAS data
CORAL model
Sample: ...regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein
Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
...Regulator FrrA. FEBS J (2021)
Werner N, Werten S, Hoppen J, Palm GJ, Göttfert M, Hinrichs W
RgGuinier 3.4 nm
Dmax 9.0 nm
VolumePorod 93 nm3

SASDKB6 – ...regulator A (FrrA) in the presence of genistein

TetR/AcrR family transcriptional regulator5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one experimental SAS data
TetR/AcrR family transcriptional regulator 5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one Kratky plot
Sample: ...regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein
5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one monomer, 0 kDa
Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, 0.08 mM genistein, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 27
...Regulator FrrA. FEBS J (2021)
Werner N, Werten S, Hoppen J, Palm GJ, Göttfert M, Hinrichs W
RgGuinier 3.4 nm
Dmax 8.7 nm
VolumePorod 96 nm3

SASDKC6 – ...regulator A (FrrA) in the presence of naringenin

TetR/AcrR family transcriptional regulator5,7-dihydroxy-2-(4-hydroxyphenyl)chroman-4-one experimental SAS data
TetR/AcrR family transcriptional regulator 5,7-dihydroxy-2-(4-hydroxyphenyl)chroman-4-one Kratky plot
Sample: ...regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein
5,7-dihydroxy-2-(4-hydroxyphenyl)chroman-4-one monomer, 0 kDa
Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, 1 mM naringenin, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
...Regulator FrrA. FEBS J (2021)
Werner N, Werten S, Hoppen J, Palm GJ, Göttfert M, Hinrichs W
RgGuinier 3.2 nm
Dmax 8.6 nm
VolumePorod 91 nm3

SASDCJ6 – ...Response regulator (fragment 20-298) from Methylococcus capsulatus str. Bath, Northeast Structural Genomics Consortium Target McR175G

MmoQ experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: MmoQ monomer, 32 kDa Methylococcus capsulatus protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
Small angle X-ray scattering as a complementary tool for high-throughput structural studies. Biopolymers 95(8):517-30 (2011)
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 62 nm3

SASDAA7 – ...response regulator

Histidine protein kinaseResponse regulator experimental SAS data
BUNCH model
Sample: Histidine protein kinase dimer, 54 kDa Streptococcus pneumoniae protein
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 4.0 nm
Dmax 16.0 nm
VolumePorod 175 nm3

SASDAB7 – Complex ComE-comcde

comcdeResponse regulator experimental SAS data
OTHER model
Sample: comcde , 24 kDa Streptococcus pneumoniae DNA
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 11
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 122 nm3

SASDAC7 – Complex LytTR-comcde

comcdeResponse regulator experimental SAS data
SASREF model
Sample: comcde , 24 kDa Streptococcus pneumoniae DNA
Response regulator dimer, 29 kDa Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Jun 2
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 74 nm3

SASDQP8 – OxyS sRNA in HEPES buffer pH 6.8, 50 mM NaCl

Stress response regulating small RNA OxyS experimental SAS data
OTHER [STATIC IMAGE] model
Sample: ...response regulating small RNA OxyS monomer, 36 kDa Escherichia coli RNA
Buffer: 50 mM HEPES pH 6.8, 50 mM NaCl, pH: 6.8
Experiment: SAXS data collected at Austrian SAXS beamline 5.2L, ELETTRA on 2022 Jan 31
...response sRNA RNA :rna.079618.123 (2023)
Stih V, Amenitsch H, Plavec J, Podbevsek P
RgGuinier 4.8 nm
Dmax 15.5 nm
VolumePorod 68 nm3

SASDCH9 – Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) with GDP

Sensory box/response regulator experimental SAS data
CORAL model
Sample: ...response regulator dimer, 136 kDa Mycobacterium smegmatis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 2 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jun 26
The GDP-switched GAF domain of DcpA modulates the concerted synthesis/hydrolysis of c-di-GMP in Mycobacterium smegmatis. Biochem J 475(7):1295-1308 (2018)
Chen HJ, Li N, Luo Y, Jiang YL, Zhou CZ, Chen Y, Li Q
RgGuinier 5.0 nm
Dmax 20.0 nm
VolumePorod 299 nm3

SASDCJ9 – Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) without GDP

Sensory box/response regulator experimental SAS data
CORAL model
Sample: ...response regulator dimer, 136 kDa Mycobacterium smegmatis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 2 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jun 26
The GDP-switched GAF domain of DcpA modulates the concerted synthesis/hydrolysis of c-di-GMP in Mycobacterium smegmatis. Biochem J 475(7):1295-1308 (2018)
Chen HJ, Li N, Luo Y, Jiang YL, Zhou CZ, Chen Y, Li Q
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 271 nm3

SASDMM9 – Response Regulator RocR from Pseudomonas aeruginosa

RocR experimental SAS data
OTHER model
Sample: RocR tetramer, 171 kDa Pseudomonas aeruginosa (strain … protein
Buffer: 50 mM Tris–HCl, 250 mM NaCl, 10 mM imidazole, 5% glycerol, 0.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 9
...Regulatory Mechanism of the Response Regulator RocR from Pseudomonas aeruginosa in Cyclic Di-GMP Signaling Journal of Bacteriology 194(18):4837-4846 (2012)
Chen M, Kotaka M, Vonrhein C, Bricogne G, Rao F, Chuah M, Svergun D, Schneider G, Liang Z, Lescar J
RgGuinier 3.7 nm
Dmax 11.0 nm