Small Angle Scattering Biological Data Bank SASBDB


Advanced search

20 hits found for Structural polyprotein

SASDPY2 – ...structural protein 7-8 (nsp7-8) polyprotein monomer

Replicase polyprotein 1ab experimental SAS data

Sample:	...polyprotein 1ab monomer, 31 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15

...structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E

R_g^Guinier	2.5	nm
D_max	8.8	nm
Volume^Porod	50	nm³

SASDPZ2 – ...structural protein 7-8 (nsp7-8) polyprotein dimer

Sample:	...polyprotein 1ab dimer, 63 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15

R_g^Guinier	3.3	nm
D_max	11.8	nm
Volume^Porod	110	nm³

SASDP23 – ...structural protein 7-11 (nsp7-11) polyprotein monomer

Replicase polyprotein 1a experimental SAS data

Sample:	...polyprotein 1a monomer, 60 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

R_g^Guinier	3.5	nm
D_max	15.6	nm
Volume^Porod	102	nm³

SASDP33 – ...structural protein 7-11 (nsp7-11) polyprotein dimer

Sample:	...polyprotein 1a dimer, 119 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14

R_g^Guinier	4.6	nm
D_max	19.1	nm
Volume^Porod	203	nm³

SASDP56 – ...structural protein 8 (nsp8, dimer) in 250 mM NaCl buffer

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) experimental SAS data

Replicase polyprotein 1a (Non-structural protein 8, SARS-CoV-2) Kratky plot

Sample:	...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2. Commun Biol 5(1):925 (2022)
Xu J, Jiang X, Zhang Y, Dong Y, Ma C, Jiang H, Zuo T, Chen R, Ke Y, Cheng H, Wang H, Liu J

R_g^Guinier

4.1

SASDP66 – ...structural protein 8 (nsp8, dimer) in 500 mM NaCl buffer

Sample:	...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	98	nm³

SASDP76 – ...structural protein 8 (nsp8, dimer) in 1000 mM NaCl buffer

Sample:	...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) dimer, 44 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	3.8	nm
D_max	14.0	nm
Volume^Porod	100	nm³

SASDP86 – ...structural protein 8 (nsp8, tetramer) in 250 mM NaCl buffer

Sample:	...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 250 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	6.3	nm
D_max	24.0	nm

SASDP96 – ...structural protein 8 (nsp8, tetramer) in 500 mM NaCl buffer

Sample:	...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 500 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	5.2	nm
D_max	18.0	nm

SASDPA6 – ...structural protein 8 (nsp8, tetramer) in 1000 mM NaCl buffer

Sample:	...polyprotein 1a (Non-structural protein 8, SARS-CoV-2) tetramer, 88 kDa Severe acute respiratory … protein
Buffer:	20 mM HEPES pH 7.4, 1000 mM NaCl, pH: 7.4
Experiment:	SANS data collected at BL01-Small Angle Neutron Scattering, China Spallation Neutron Source on 2021 Jul 19

R_g^Guinier	4.8	nm
D_max	16.0	nm

SASDUS6 – Amino-terminal domain of Infectious Bursal Disease Virus VP3 at pH8.0

Structural polyprotein experimental SAS data

Sample:	Structural polyprotein dimer, 26 kDa Infectious bursal disease … protein
Buffer:	50 mM Tris, 500 mM NaCl, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Sep 27

Infectious Bursal Disease Virus VP3
Diego Ferrero

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	52	nm³

SASDKT6 – ...structural protein 14 (nsp14)

Replicase polyprotein 1ab (non-structural protein 14) experimental SAS data

Sample:	...polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...

R_g^Guinier	2.7	nm
D_max	9.6	nm
Volume^Porod	80	nm³

SASDKU6 – ...structural protein 10/non-structural protein 14 complex (nsp10/nsp14 )

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10) experimental SAS data

Sample:	...polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein ...polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

R_g^Guinier	2.9	nm
D_max	12.2	nm
Volume^Porod	73	nm³

SASDKV6 – ...structural protein 10/non-structural protein 16 complex (nsp10/nsp16)

Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data

Sample:	...polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein ...polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

R_g^Guinier	2.0	nm
D_max	8.0	nm
Volume^Porod	28	nm³

SASDKW6 – ...structural protein 10/non-structural protein 14/non-structural protein 16 triplex (nsp10/nsp14/nsp16)

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data

Sample:	...polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein ...polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein ...polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer:	50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Dec 11

R_g^Guinier	4.7	nm
D_max	11.4	nm
Volume^Porod	113	nm³

SASDS69 – ...structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot

Sample:	...polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Nov 25

...Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F

R_g^Guinier	1.7	nm
D_max	5.9	nm
Volume^Porod	21	nm³

SASDS79 – ...structural protein 10 (Nsp10: C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) Kratky plot

Sample:	...polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Nov 25

R_g^Guinier	1.6	nm
D_max	5.4	nm
Volume^Porod	19	nm³

SASDS89 – ...structural protein 10 (Nsp10: N- and C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) experimental SAS data

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) Kratky plot

Sample:	...polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Nov 25

R_g^Guinier	1.5	nm
D_max	5.1	nm
Volume^Porod	16	nm³

SASDS99 – ...structural protein 10 (Nsp10)

Sample:	...polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 6

R_g^Guinier	1.7	nm
D_max	5.9	nm
Volume^Porod	19	nm³

SASDSA9 – ...structural protein 10 (Nsp10)

Sample:	...polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
Buffer:	10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Feb 6

R_g^Guinier	1.7	nm
D_max	5.6	nm
Volume^Porod	19	nm³

Search