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14 hits found for saposin-a

SASDCX6 – T2 SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineMechanosensitive channel T2 experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine , 47 kDa synthetic construct
Mechanosensitive channel T2 heptamer, 230 kDa Thermoplasma volcanium protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 5.4 nm
Dmax 18.0 nm
VolumePorod 686 nm3

SASDCY6 – T2 in b-DDM micelle (SEC-SAXS)

Mechanosensitive channel T2n-Dodecyl-β-D-Maltopyranoside experimental SAS data
DAMMIF model
Sample: Mechanosensitive channel T2 heptamer, 230 kDa Thermoplasma volcanium protein
n-Dodecyl-β-D-Maltopyranoside , 77 kDa synthetic construct
Buffer: 20 mM Tris 150 mM NaCl 5 % glycerol 0.03 % DDM, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 May 29
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 5.6 nm
Dmax 16.5 nm
VolumePorod 896 nm3

SASDCZ6 – DtpA SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineDipeptide and tripeptide permease A experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine , 47 kDa synthetic construct
Dipeptide and tripeptide permease A monomer, 51 kDa Escherichia coli protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 288 nm3

SASDC27 – Saposin nanoparticle (SapNP) SapA:DOPC

saposin-a1,2-dioleoyl-sn-glycero-3-phosphocholine experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
1,2-dioleoyl-sn-glycero-3-phosphocholine , 47 kDa synthetic construct
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.1 nm
Dmax 11.5 nm
VolumePorod 96 nm3

SASDC37 – Saposin nanoparticle (SapNP) SapA:SoyPI

saposin-aL-α-phosphatidylinositol (soy) experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
L-α-phosphatidylinositol (soy) , 52 kDa Glycine max
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 3.6 nm
Dmax 8.5 nm
VolumePorod 68 nm3

SASDC47 – Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) experimental SAS data
DAMMIF model
Sample: saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) , 46 kDa synthetic construct
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 15
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.1 nm
Dmax 10.5 nm
VolumePorod 69 nm3

SASDJL9 – Saposin-A monomer

Prosaposin (Saposin A) experimental SAS data
Prosaposin (Saposin A) Kratky plot
Sample: Prosaposin (Saposin A) monomer, 10 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 1.5 nm
Dmax 5.0 nm
VolumePorod 23 nm3

SASDJM9 – Saposin-A plus MNA C12 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol)

Prosaposin (Saposin-A)1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol experimental SAS data
Prosaposin (Saposin-A) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol Kratky plot
Sample: ...Saposin-A) dimer, 19 kDa Homo sapiens protein
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol decamer, 12 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.3 nm
Dmax 6.2 nm

SASDJN9 – Saposin-A plus MNA C13 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol)

Prosaposin (Saposin-A)1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol experimental SAS data
Prosaposin (Saposin-A) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol Kratky plot
Sample: ...Saposin-A) dimer, 19 kDa Homo sapiens protein
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol decamer, 12 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.5 nm
Dmax 6.7 nm

SASDJP9 – Saposin-A plus LMNG (2,2-didecylpropane-1,3-bis-β-D-maltopyranoside)

Prosaposin (Saposin-A)2,2-didecylpropane-1,3-bis-β-D-maltopyranoside experimental SAS data
Prosaposin (Saposin-A) 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside Kratky plot
Sample: ...Saposin-A) dimer, 19 kDa Homo sapiens protein
2,2-didecylpropane-1,3-bis-β-D-maltopyranoside 0, 15 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.5 nm
Dmax 7.0 nm

SASDJQ9 – Saposin-B, R232W mutant

Prosaposin (Saposin-B, R232W mutant) experimental SAS data
Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.0 nm
Dmax 5.8 nm

SASDJR9 – Saposin-B, R232W mutant, plus MNA C12 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol)

1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitolProsaposin (Saposin-B, R232W mutant) experimental SAS data
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol decamer, 12 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.3 nm
Dmax 6.9 nm

SASDJS9 – Saposin-B, R232W mutant, plus MNA C13 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol)

1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitolProsaposin (Saposin-B, R232W mutant) experimental SAS data
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol decamer, 12 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.6 nm
Dmax 7.7 nm

SASDJT9 – Saposin-B, R232W mutant, plus LMNG (2,2-didecylpropane-1,3-bis-β-D-maltopyranoside)

2,2-didecylpropane-1,3-bis-β-D-maltopyranosideProsaposin (Saposin-B, R232W mutant) experimental SAS data
2,2-didecylpropane-1,3-bis-β-D-maltopyranoside Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside 0, 15 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.8 nm
Dmax 8.9 nm