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66 hits found for benchmark

SASDG52 – 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 30 nm filter extrusion

1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
Konarev P, Gruzinov A, Mertens H, Svergun D
Dmax 66.0 nm

SASDG62 – 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 50 nm filter extrusion

1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
Konarev P, Gruzinov A, Mertens H, Svergun D
Dmax 81.5 nm

SASDG72 – 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 100 nm filter extrusion

1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
Konarev P, Gruzinov A, Mertens H, Svergun D
Dmax 111.0 nm

SASDG82 – 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 200 nm filter extrusion

1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
Konarev P, Gruzinov A, Mertens H, Svergun D
Dmax 147.0 nm

SASDG92 – 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles: 30 nm filter extrusion

1,2-dipalmitoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
Konarev P, Gruzinov A, Mertens H, Svergun D
Dmax 66.0 nm

SASDGA2 – 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles: 50 nm filter extrusion

1,2-dipalmitoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
Konarev P, Gruzinov A, Mertens H, Svergun D
Dmax 105.0 nm

SASDAB2 – Cytochrome c from equine heart

Cytochrome cHeme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cytochrome c monomer, 12 kDa Equus caballus protein
Heme C monomer, 1 kDa
Buffer: 25 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Apr 8
Standard proteins
Cy M Jeffries
RgGuinier 1.3 nm
Dmax 3.7 nm
VolumePorod 12 nm3

SASDGB2 – 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles: 100 nm filter extrusion

1,2-dipalmitoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
Konarev P, Gruzinov A, Mertens H, Svergun D
Dmax 112.0 nm

SASDGC2 – 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles: 200 nm filter extrusion

1,2-dipalmitoyl-sn-glycero-3-phosphocholine experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 9
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data Journal of Applied Crystallography 54(1) (2021)
Konarev P, Gruzinov A, Mertens H, Svergun D
Dmax 180.0 nm

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...
RgGuinier 3.3 nm
Dmax 9.2 nm
VolumePorod 229 nm3

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

Calmodulin-1 experimental SAS data
4Ca2+-calmodulin - Xenopus laevis Rg histogram
Sample: Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...
RgGuinier 2.2 nm
Dmax 7.2 nm
VolumePorod 25 nm3

SASDPH3 – Benchmarking extraction methods of scattering patterns of poorly resolved components from a SEC-SAXS dataset of BSA.

Bovine Serum Albumin experimental SAS data
Bovine Serum Albumin Kratky plot
Sample: Bovine Serum Albumin trimer, 199 kDa Bos taurus protein
Buffer: 20 mM Hepes, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Sep 24
SEC-SAXS: Experimental set-up and software developments build up a powerful tool. Methods Enzymol 677:221-249 (2022)
Pérez J, Thureau A, Vachette P
RgGuinier 5.2 nm
Dmax 17.6 nm
VolumePorod 370 nm3

SASDCJ3 – Bovine Serum Albumin

Serum albumin experimental SAS data
DAMMIN model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...
RgGuinier 2.8 nm
Dmax 8.7 nm
VolumePorod 101 nm3

SASDMV3 – Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm

Nominal 100 nm diameter polystyrene spheres experimental SAS data
BODIES model
Sample: Nominal 100 nm diameter polystyrene spheres monomer, 315312 kDa
Buffer: Water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 16
Standard polystyrene nanospheres (concentration series data)
Dima Molodenskiy
RgGuinier 33.0 nm
Dmax 100.0 nm
VolumePorod 520 nm3

SASDMW3 – Thermo Fisher polystyrene nanospheres with a diameter of 20 nm

Nominal 20 nm diameter polystyrene spheres experimental SAS data
BODIES model
Sample: Nominal 20 nm diameter polystyrene spheres 0, 8 kDa
Buffer: Water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 16
Standard polystyrene nanospheres (concentration series data)
Dima Molodenskiy
RgGuinier 8.5 nm
Dmax 25.0 nm
VolumePorod 3 nm3

SASDUA4 – Ribonuclease A Updated Consensus SAXS Data

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 1.5 nm
Dmax 5.0 nm

SASDUB4 – Urate Oxidase Updated Consensus SAXS Data

Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 3.2 nm
Dmax 9.3 nm

SASDUC4 – Xylose Isomerase Updated Consensus SAXS Data

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 3.3 nm
Dmax 10.1 nm

SASDUD4 – Xylanase Updated Consensus SAXS Data

Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 1.6 nm
Dmax 5.2 nm

SASDUE4 – Lysozyme Updated Consensus SAXS Data

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 1.5 nm
Dmax 4.7 nm

SASDPP4 – Consensus SAXS Profile - Ribonuclease A

Ribonuclease pancreatic experimental SAS data
DAMMIN model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.5 nm
Dmax 4.9 nm
VolumePorod 18 nm3

SASDPQ4 – Consensus SAXS Profile - Urate Oxidase

Uricase experimental SAS data
DAMMIN model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 3.2 nm
Dmax 9.2 nm
VolumePorod 220 nm3

SASDPR4 – Consensus SAXS Profile - Xylose Isomerase

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: ConsensusBuffer_50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 3.3 nm
Dmax 10.1 nm
VolumePorod 243 nm3

SASDPS4 – Consensus SAXS Profile - Xylanase

Endo-1,4-beta-xylanase experimental SAS data
DAMMIN model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.6 nm
Dmax 5.1 nm
VolumePorod 27 nm3

SASDBT4 – Bovine serum albumin monomer (measured by SEC-SAXS)

Bovine serum albumin, monomer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 20 mM Tris 142 mM NaCl 5 % Glycerol 1mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Bovine Serum Albumin measured by SEC-SAXS
Martha Brennich
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 118 nm3

SASDPT4 – Consensus SAXS Profile - Lysozyme

Lysozyme C experimental SAS data
DAMMIN model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 19 nm3

SASDPU4 – Consensus SANS Profile - Ribonuclease A in 100% v/v D2O buffer

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.4 nm
Dmax 4.4 nm

SASDPV4 – Consensus SANS Profile - Lysozyme in 100% v/v D2O buffer

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.2 nm
Dmax 3.8 nm

SASDPW4 – Consensus SANS Profile - Xylanase in 100% v/v D2O buffer

Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.5 nm
Dmax 4.4 nm

SASDPX4 – Consensus SANS Profile - Urate oxidase in 100% v/v D2O buffer

Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 3.1 nm
Dmax 9.3 nm

SASDPY4 – Consensus SANS Profile - Xylose isomerase in 100% v/v D2O buffer

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 3.1 nm
Dmax 9.5 nm

SASDPZ4 – Consensus SANS Profile - Lysozyme in H2O buffer

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.4 nm
Dmax 4.8 nm

SASDP25 – Consensus SANS Profile - Ribonuclease A in H2O buffer

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.5 nm
Dmax 4.1 nm

SASDP35 – Consensus SANS Profile - Xylanase in H2O buffer

Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 1.6 nm
Dmax 4.3 nm

SASDP45 – Consensus SANS Profile - Urate oxidase in H2O buffer

Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 3.2 nm
Dmax 9.1 nm

SASDP55 – Consensus SANS Profile - Xylose isomerase in H2O buffer

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24
...benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...
RgGuinier 3.3 nm
Dmax 9.7 nm

SASDA96 – Lysozyme

Lysozyme C experimental SAS data
DAMMIF model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 20 mM Sodium Acetate/HEPES, pH: 6.8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Feb 17
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 24 nm3

SASDAA6 – Human serum albumin monomer and mixtures

Human serum albumin monomer experimental SAS data
DAMMIF model
Sample: Human serum albumin monomer monomer, 66 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 22
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 103 nm3

SASDAB6 – Xylose Isomerase

Xylose Isomerase experimental SAS data
DAMMIF model
Sample: Xylose Isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 20 mM HEPES 200 mM Na2SO4 50 mM K2SO4 500 % v/v D2O 1 mM MgCl2, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 10
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 3.2 nm
Dmax 9.1 nm
VolumePorod 234 nm3

SASDPF7 – Ribonuclease A SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16
...benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.6 nm
VolumePorod 16 nm3

SASDPG7 – Ribonuclease A SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Ribonuclease pancreatic experimental SAS data
Ribonuclease pancreatic Kratky plot
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
...benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 16 nm3

SASDPH7 – Urate Oxidase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15
...benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 8.8 nm
VolumePorod 221 nm3

SASDPJ7 – Urate Oxidase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Urate Oxidase (Uricase) from Aspergillus flavus experimental SAS data
Urate Oxidase (Uricase) from Aspergillus flavus Kratky plot
Sample: Urate Oxidase (Uricase) from Aspergillus flavus tetramer, 137 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, 1 % v/v glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
...benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 9.0 nm
VolumePorod 214 nm3

SASDPK7 – Xylose isomerase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 15
...benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.2 nm
Dmax 14.1 nm
VolumePorod 228 nm3

SASDPL7 – Xylose isomerase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, 1 % v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
...benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 3.3 nm
Dmax 14.1 nm
VolumePorod 233 nm3

SASDPM7 – Xylanase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16
...benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.6 nm
Dmax 4.8 nm
VolumePorod 25 nm3

SASDPN7 – Xylanase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Endo-1,4-beta-xylanase experimental SAS data
Endo-1,4-beta-xylanase Kratky plot
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8
...benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun
RgGuinier 1.6 nm
Dmax 4.5 nm
VolumePorod 24 nm3

SASDCD8 – Horse spleen apoferritin (WAXS)

Horse spleen apoferritin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Horse spleen apoferritin 24-mer, 476 kDa Equus caballus protein
Buffer: tbs, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 15
...benchmark on standard proteins
Maxim Petoukhov
RgGuinier 6.3 nm

SASDCE8 – Beta amylase from sweet potato (WAXS)

Beta-amylase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Beta-amylase tetramer, 224 kDa Ipomoea batatas protein
Buffer: tbs, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 15
...benchmark on standard proteins
Maxim Petoukhov
RgGuinier 4.0 nm

SASDCF8 – Bovine serum albumin (WAXS)

Serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: Hepes, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 25
...benchmark on standard proteins
Maxim Petoukhov
RgGuinier 2.7 nm

SASDCG8 – Bovine carbonic anhydrase (WAXS)

Carbonic anhydrase 2 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: Tbs, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 16
...benchmark on standard proteins
Maxim Petoukhov
RgGuinier 1.9 nm

SASDCH8 – Horse heart cytochrome C (WAXS)

Cytochrome c experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cytochrome c monomer, 12 kDa Equus caballus protein
Buffer: tbs, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jul 16
...benchmark on standard proteins
Maxim Petoukhov
RgGuinier 1.2 nm

SASDCJ8 – Glucose isomerase (WAXS)

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer: 100 mM tris pH 8.0, 100 mM NaCl, 1 mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 17
...benchmark on standard proteins
Maxim Petoukhov
RgGuinier 3.2 nm

SASDCK8 – Lysozyme (WAXS)

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM Sodium Acetate pH 4.0, 50 mM NaCl, pH: 4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 May 31
...benchmark on standard proteins
Maxim Petoukhov
RgGuinier 1.4 nm

SASDCL8 – Horse myoglobin (WAXS)

Myoglobin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Myoglobin monomer, 17 kDa Equus caballus protein
Buffer: 100 mM tris pH 7.5, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
...benchmark on standard proteins
Maxim Petoukhov
RgGuinier 1.6 nm

SASDCQ8 – Bovine serum albumin (BSA)

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.8 nm

SASDCR8 – Glucose isomerase

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 100 mM HEPES, 1 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.7 nm

SASDCS8 – Cytochrome C

Cytochrome C experimental SAS data
Cytochrome C Kratky plot
Sample: Cytochrome C monomer, 12 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 1.3 nm

SASDAA8 – Chymotrypsinogen A in Tris/HCl

Chymotrypsinogen A experimental SAS data
CRYSOL model
Sample: Chymotrypsinogen A monomer, 26 kDa Bos taurus protein
Buffer: 100 mM Tris/HCl 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 19
Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering Journal of Applied Crystallography 40(s1):s245-s249 (2007)
Mylonas E, Svergun D
RgGuinier 1.9 nm
Dmax 5.0 nm

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data
DAMMIN model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 1.8 nm
Dmax 5.1 nm
VolumePorod 37 nm3

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 98 nm3

SASDA68 – Aldolase in Tris/HCl

Fructose-bisphosphate aldolase A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Fructose-bisphosphate aldolase A tetramer, 157 kDa Oryctolagus cuniculus protein
Buffer: 100 mM Tris/HCl 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 19
Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering Journal of Applied Crystallography 40(s1):s245-s249 (2007)
Mylonas E, Svergun D
RgGuinier 3.6 nm
Dmax 10.5 nm

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 4.0 nm
Dmax 13.2 nm
VolumePorod 211 nm3

SASDFS8 – Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data
DAMMIN model
Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 3.3 nm
Dmax 9.3 nm
VolumePorod 201 nm3

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data
DAMMIN model
Sample: Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 5.4 nm
Dmax 12.5 nm
VolumePorod 679 nm3

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 95 nm3