Small Angle Scattering Biological Data Bank SASBDB


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187 hits found for Pollack

SASDKF2 – Mixed-sequence DNA dimer (25 base pair DNA)

Mixed-sequence 25 base-paired DNA experimental SAS data

Mixed-sequence 25 base-paired DNA Kratky plot

Sample:	Mixed-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer:	1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

The structural plasticity of nucleic acid duplexes revealed by WAXS and MD. Sci Adv 7(17) (2021)
...Pollack L, Kirmizialtin S

R_g^Guinier	2.0	nm
D_max	7.8	nm
Volume^Porod	17	nm³

SASDHG2 – 12 base-paired RNA double helix (RNA12) with 30 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data

12 base-paired RNA double helix Kratky plot

Sample:	12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer:	30 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

1.4

SASDKG2 – AT-sequence DNA dimer (25 base pair DNA)

AT-sequence 25 base-paired DNA experimental SAS data

AT-sequence 25 base-paired DNA Kratky plot

Sample:	AT-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer:	1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

The structural plasticity of nucleic acid duplexes revealed by WAXS and MD. Sci Adv 7(17) (2021)
...Pollack L, Kirmizialtin S

R_g^Guinier	2.1	nm
D_max	8.0	nm
Volume^Porod	16	nm³

SASDHH2 – 12 base-paired RNA double helix (RNA12) with 50 mM KCl - SWAXS

Sample:	12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer:	50 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

1.4

SASDHJ2 – 12 base-paired RNA double helix (RNA12) with 100 mM KCl - SWAXS

Sample:	12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer:	100 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

1.5

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
...Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P, Westbrook J, Whitten AE

R_g^Guinier	3.3	nm
D_max	9.2	nm
Volume^Porod	229	nm³

SASDHK2 – 12 base-paired RNA double helix (RNA12) with 200 mM KCl - SWAXS

Sample:	12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer:	200 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

1.6

SASDHL2 – 12 base-paired RNA double helix (RNA12) with 500 mM KCl - SWAXS

Sample:	12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer:	500 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

2.1

SASDHM2 – 12 base-paired RNA double helix (RNA12) with 0.25 mM MgCl2 - SWAXS

Sample:	12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer:	0.25 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

1.4

SASDHN2 – 12 base-paired RNA double helix (RNA12) with 1 mM MgCl2 - SWAXS

Sample:	12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer:	1.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

1.6

SASDHP2 – 12 base-paired RNA double helix (RNA12) with 5 mM MgCl2 - SWAXS

Sample:	12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer:	5.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

2.0

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

4Ca2+-calmodulin - Xenopus laevis Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	25	nm³

SASDHQ2 – 25 base-paired RNA double helix (RNA25) with 100 mM NaCl - WAXS

25 base-paired RNA double helix experimental SAS data

25 base-paired RNA double helix Kratky plot

Sample:	25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer:	100 mM NaCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

2.1

SASDHR2 – 25 base-paired RNA double helix (RNA25) with 10 mM MgCl2 - WAXS

Sample:	25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer:	10.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

2.6

SASDHS2 – 25 base-paired RNA double helix (RNA25) with 400 mM KCl - WAXS

Sample:	25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer:	400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

2.3

SASDHT2 – 25 base-paired DNA double helix (DNA25) with 400 mM KCl - SWAXS

25 base-paired DNA double helix experimental SAS data

25 base-paired DNA double helix Kratky plot

Sample:	25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer:	400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

1.9

SASDKT2 – Wild type double Element for Nuclear Expression (dENE)

Sample:	Wild type dENE monomer, 23 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	2.8	nm
D_max	10.8	nm
Volume^Porod	31	nm³

SASDHU2 – 25 base-paired DNA double helix (DNA25) with 0.5 mM MgCl2 - SWAXS

Sample:	25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer:	0.5 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

2.0

SASDKU2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)20

Wild type dENE + Poly(A)20 experimental SAS data

Sample:	Wild type dENE + Poly(A)20 monomer, 30 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	2.8	nm
D_max	10.7	nm

SASDHV2 – 25 base-paired DNA double helix (DNA25) with 2 mM MgCl2 - SWAXS

Sample:	25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer:	2.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16

Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
...Pollack L

R_g^Guinier

1.9

SASDKV2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)25

Wild type dENE + Poly(A)25 experimental SAS data

Sample:	Wild type dENE + Poly(A)25 monomer, 31 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	2.9	nm
D_max	10.4	nm

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30 experimental SAS data

Sample:	Wild type dENE + Poly(A)30 monomer, 33 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	2.9	nm
D_max	10.6	nm

SASDKX2 – double Element for Nuclear Expression (dENE) Mutant A

Sample:	A dENE monomer, 27 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.2	nm
D_max	10.9	nm

SASDKY2 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)20

A dENE + Poly(A)20 experimental SAS data

Sample:	A dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.2	nm
D_max	11.5	nm

SASDK23 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)30

A dENE + Poly(A)30 experimental SAS data

Sample:	A dENE + Poly(A)30 monomer, 37 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.3	nm
D_max	13.3	nm

SASDK33 – double Element for Nuclear Expression (dENE) Mutant B

Sample:	B dENE monomer, 24 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	2.9	nm
D_max	10.8	nm

SASDK43 – double Element for Nuclear Expression (dENE) Mutant B + Poly(A)30

B dENE + Poly(A)30 experimental SAS data

Sample:	B dENE + Poly(A)30 monomer, 34 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.2	nm
D_max	11.8	nm

SASDK63 – double Element for Nuclear Expression (dENE) Mutant C

Sample:	C dENE monomer, 29 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.4	nm
D_max	12.4	nm

SASDK73 – double Element for Nuclear Expression (dENE) Crystal Structure

Sample:	Xtal dENE monomer, 28 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.1	nm
D_max	11.0	nm

SASDK83 – double Element for Nuclear Expression (dENE) Mutant C + Poly(A)30

C dENE + Poly(A)30 experimental SAS data

Sample:	C dENE + Poly(A)30 monomer, 39 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.6	nm
D_max	14.8	nm

SASDKA3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)25

Xtal dENE + Poly(A)25 experimental SAS data

Sample:	Xtal dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.3	nm
D_max	12.4	nm

SASDKB3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)30

Xtal dENE + Poly(A)30 experimental SAS data

Sample:	Xtal dENE + Poly(A)30 monomer, 38 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.5	nm
D_max	13.2	nm

SASDKC3 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)25

A dENE + Poly(A)25 experimental SAS data

Sample:	A dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.3	nm
D_max	12.4	nm

SASDKD3 – double Element for Nuclear Expression (dENE) Mutant B + Hairpin - Poly(A)30

B dENE + Hairpin - Poly(A)30 experimental SAS data

B dENE + Hairpin - Poly(A)30 Kratky plot

Sample:	B dENE + Hairpin - Poly(A)30 monomer, 39 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.5	nm
D_max	14.0	nm

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20 experimental SAS data

Sample:	Xtal dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer:	50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5

Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Pollack L, Steitz J

R_g^Guinier	3.2	nm
D_max	11.8	nm

SASDRE3 – Tissue Transglutaminase + Ca: Time-resolved 0ms

Protein-glutamine gamma-glutamyltransferase 2 experimental SAS data

Protein-glutamine gamma-glutamyltransferase 2 Kratky plot

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	4.1	nm
D_max	16.0	nm
Volume^Porod	150	nm³

SASDRF3 – Tissue Transglutaminase + Ca: Time-resolved 32 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	4.1	nm
D_max	17.0	nm
Volume^Porod	170	nm³

SASDRG3 – Tissue Transglutaminase + Ca: Time-resolved 63 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	4.1	nm
D_max	18.0	nm
Volume^Porod	155	nm³

SASDRH3 – Tissue Transglutaminase + Ca: Time-resolved 100 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	4.1	nm
D_max	20.0	nm
Volume^Porod	190	nm³

SASDCJ3 – Bovine Serum Albumin

Sample:	Serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

R_g^Guinier	2.8	nm
D_max	8.7	nm
Volume^Porod	101	nm³

SASDRJ3 – Tissue Transglutaminase + Ca: Time-resolved 316 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	4.5	nm
D_max	24.0	nm
Volume^Porod	200	nm³

SASDRK3 – Tissue Transglutaminase + Ca: Time-resolved 631 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	4.6	nm
D_max	26.0	nm
Volume^Porod	215	nm³

SASDRL3 – Tissue Transglutaminase + Ca: Time-resolved 1500 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	4.6	nm
D_max	28.0	nm
Volume^Porod	230	nm³

SASDTL3 – Tissue Transglutaminase: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.2	nm
D_max	20.0	nm
Volume^Porod	148	nm³

SASDRM3 – Tissue Transglutaminase + Ca: 5min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	5.0	nm
D_max	27.0	nm
Volume^Porod	241	nm³

SASDTM3 – Tissue Transglutaminase + 25 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.8	nm
D_max	17.5	nm
Volume^Porod	132	nm³

SASDRN3 – Tissue Transglutaminase + Ca: 10min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Nov 19

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	5.5	nm
D_max	26.0	nm
Volume^Porod	300	nm³

SASDTN3 – Tissue Transglutaminase + 50 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.7	nm
D_max	17.5	nm
Volume^Porod	124	nm³

SASDRP3 – Tissue Transglutaminase + Ca: 30min Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2021 Sep 29

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	6.9	nm
D_max	31.5	nm
Volume^Porod	490	nm³

SASDTP3 – Tissue Transglutaminase + 100 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.6	nm
D_max	18.0	nm
Volume^Porod	121	nm³

SASDRQ3 – Trypsin + Aprotinin: Time-Resolved 10 ms

Serine protease 1Pancreatic trypsin inhibitor experimental SAS data

Serine protease 1 Pancreatic trypsin inhibitor Kratky plot

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	23	nm³

SASDTQ3 – Tissue Transglutaminase + 250 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.5	nm
D_max	17.5	nm
Volume^Porod	120	nm³

SASDRR3 – Trypsin + Aprotinin: Time-Resolved 30 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	28	nm³

SASDTR3 – Tissue Transglutaminase + 500 µM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.4	nm
D_max	18.0	nm
Volume^Porod	118	nm³

SASDRS3 – Trypsin + Aprotinin: Time-Resolved 100 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	1.8	nm
D_max	5.3	nm
Volume^Porod	30	nm³

SASDTS3 – Tissue Transglutaminase + 1 mM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.4	nm
D_max	17.0	nm
Volume^Porod	115	nm³

SASDRT3 – Trypsin + Aprotinin: Time-Resolved 400 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	27	nm³

SASDTT3 – Tissue Transglutaminase + 2 mM GTP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.3	nm
D_max	17.0	nm
Volume^Porod	111	nm³

SASDRU3 – Trypsin + Aprotinin: Time-Resolved 630 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	25	nm³

SASDTU3 – Tissue Transglutaminase + 5 mM GTP: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM GTP, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.2	nm
D_max	14.0	nm
Volume^Porod	108	nm³

SASDRV3 – Trypsin + Aprotinin: Time-Resolved 1000 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	1.8	nm
D_max	5.3	nm
Volume^Porod	28	nm³

SASDTV3 – Tissue Transglutaminase + 5 mM GDP

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.5	nm
D_max	17.5	nm
Volume^Porod	120	nm³

SASDRW3 – Trypsin + Aprotinin: Time-Resolved 2000 ms

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.0	nm
D_max	5.3	nm
Volume^Porod	29	nm³

SASDTW3 – Tissue Transglutaminase + 250 µM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.3	nm
D_max	21.0	nm
Volume^Porod	160	nm³

SASDRX3 – Trypsin + Aprotinin: Complex equilibrium

Sample:	Serine protease 1 monomer, 26 kDa Bos taurus protein Pancreatic trypsin inhibitor monomer, 11 kDa Bos taurus protein
Buffer:	20 mM TRIS, 40 mM KCl, 20 mM CaCl2, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	31	nm³

SASDTX3 – Tissue Transglutaminase + 500 µM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.3	nm
D_max	20.0	nm
Volume^Porod	158	nm³

SASDRY3 – Kenics GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.2	nm
D_max	10.0	nm
Volume^Porod	28	nm³

SASDTY3 – Tissue Transglutaminase + 1 mM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.5	nm
D_max	24.0	nm
Volume^Porod	180	nm³

SASDRZ3 – Kenics GAC rRNA + Mg: Time-Resolved 32 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.2	nm
D_max	10.0	nm
Volume^Porod	27	nm³

SASDTZ3 – Tissue Transglutaminase + 2 mM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	5.3	nm
D_max	25.5	nm
Volume^Porod	234	nm³

SASDR24 – Kenics GAC rRNA + Mg: Time-Resolved 63 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.2	nm
D_max	9.5	nm
Volume^Porod	27	nm³

SASDT24 – Tissue Transglutaminase + 50 µM inhibitor LM11

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.3	nm
D_max	20.0	nm
Volume^Porod	158	nm³

SASDR34 – Kenics GAC rRNA + Mg: Time-Resolved 100 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.2	nm
D_max	10.0	nm
Volume^Porod	28	nm³

SASDT34 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 250 µM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.2	nm
D_max	19.0	nm
Volume^Porod	155	nm³

SASDR44 – Kenics GAC rRNA + Mg: Time-Resolved 316 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.2	nm
D_max	12.0	nm
Volume^Porod	27	nm³

SASDT44 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 500 µM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.9	nm
D_max	25.0	nm
Volume^Porod	191	nm³

SASDR54 – Kenics GAC rRNA + Mg: Time-Resolved 631 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.3	nm
D_max	9.8	nm
Volume^Porod	26	nm³

SASDT54 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 1 mM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.7	nm
D_max	28.0	nm
Volume^Porod	198	nm³

SASDR64 – Kenics GAC rRNA + Mg: Time-Resolved 1000 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.3	nm
D_max	11.0	nm
Volume^Porod	26	nm³

SASDT64 – Tissue Transglutaminase + 50 µM inhibitor LM11 + 2 mM CaCl2

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	5.2	nm
D_max	25.0	nm
Volume^Porod	236	nm³

SASDC74 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 0ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data

Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L

R_g^Guinier	2.5	nm
D_max	8.0	nm

SASDR74 – Kenics GAC rRNA + Mg: Equilibrium initial

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	32	nm³

SASDT74 – Tissue Transglutaminase + CaCl2 + inhibitor LM11: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM LM11, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	6.0	nm
D_max	36.0	nm
Volume^Porod	431	nm³

SASDR84 – Kenics GAC rRNA + Mg: Equilibrium final

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.2	nm
D_max	10.0	nm
Volume^Porod	26	nm³

SASDT84 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 100 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.6	nm
D_max	32.0	nm
Volume^Porod	242	nm³

SASDC94 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 10ms

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L

R_g^Guinier	2.3	nm
D_max	7.2	nm

SASDR94 – GAC rRNA + L11 Protein: Time-Resolved 30 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.4	nm
D_max	12.0	nm
Volume^Porod	34	nm³

SASDT94 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 316 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.8	nm
D_max	25.0	nm
Volume^Porod	203	nm³

SASDCA4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 30ms

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L

R_g^Guinier	2.2	nm
D_max	7.0	nm

SASDRA4 – GAC rRNA + L11 Protein: Time-Resolved 50 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.5	nm
D_max	13.5	nm
Volume^Porod	36	nm³

SASDTA4 – Tissue Transglutaminase + inhibitor LM11 + CaCl2: Time-resolved 631 ms

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	5.7	nm
D_max	33.0	nm
Volume^Porod	305	nm³

SASDCB4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 100ms

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L

R_g^Guinier	2.3	nm
D_max	7.1	nm

SASDRB4 – GAC rRNA + L11 Protein: Time-Resolved 63 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.4	nm
D_max	11.5	nm
Volume^Porod	33	nm³

SASDTB4 – Tissue Transglutaminase + CaCl2 + inhibitor LM11 + GTP: Equilibrium

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, 2 mM CaCl2, 50 µM LM11, 5 mM GTP, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	6.2	nm
D_max	33.0	nm
Volume^Porod	277	nm³

SASDCC4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 300ms

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L

R_g^Guinier	2.2	nm
D_max	6.8	nm

SASDRC4 – GAC rRNA + L11 Protein: Time-Resolved 100 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.4	nm
D_max	11.5	nm
Volume^Porod	35	nm³

SASDTC4 – Tissue Transglutaminase + 50 µM inhibitor 5826 (1% DMSO)

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	4.1	nm
D_max	19.0	nm
Volume^Porod	144	nm³

SASDCD4 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 1000ms

Sample:	Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer:	10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3

Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
...Pollack L

R_g^Guinier	2.2	nm
D_max	6.8	nm

SASDRD4 – GAC rRNA + L11 Protein: Time-Resolved 200 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer:	10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
...Pollack L

R_g^Guinier	2.5	nm
D_max	12.5	nm
Volume^Porod	37	nm³

SASDTD4 – Tissue Transglutaminase + 50 µM inhibitor 5826 (2% DMSO)

Sample:	Protein-glutamine gamma-glutamyltransferase 2 , 77 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 10% glycerol, 1 mM DTT, pH: 7.5
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Nov 17

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death. Commun Biol 7(1):982 (2024)
...Pollack L, Cerione RA, Milano SK

R_g^Guinier	3.9	nm
D_max	18.5	nm
Volume^Porod	134	nm³

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