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23 hits found for Reiners

SASDJR3 – BceAB-type ABC-Transporter nucleotide-binding domain, SaNsrF

ABC transporter, ATP-binding protein (Nucleotide-Binding Domain SaNsrF) experimental SAS data
OTHER model
Sample: ABC transporter, ATP-binding protein (Nucleotide-Binding Domain SaNsrF) monomer, 31 kDa Streptococcus agalactiae protein
Buffer: 100 mM HEPES, 150 mM NaCl, 10% glycerol, pH: 8
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2019 Dec 11
Characterization of the nucleotide-binding domain NsrF from the BceAB-type ABC-transporter NsrFP from the human pathogen Streptococcus agalactiae Scientific Reports 10(1) (2020)
...Reiners J, Schumacher J, Gottstein J, Gohlke H, Smits S
RgGuinier 2.4 nm
Dmax 7.9 nm
VolumePorod 64 nm3

SASDNM5 – Skp-like protein, SKP

Skp-like protein experimental SAS data
Skp-like protein, SKP Rg histogram
Sample: Skp-like protein trimer, 55 kDa Pseudomonas aeruginosa (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 10 % glycerol, pH: 8
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2021 Feb 26
The periplasmic chaperone Skp prevents misfolding of the secretory lipase A from Pseudomonas aeruginosa Frontiers in Molecular Biosciences 9 (2022)
...Reiners J, Hachani E, Baeumers M, Berger J, Schmitt L, Jaeger K, Kovacic F, Smits S, Kedrov A
RgGuinier 3.5 nm
Dmax 11.1 nm
VolumePorod 172 nm3

SASDTQ5 – SemDΔAPH (apo form)

Uncharacterized protein experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
...Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 67 nm3

SASDTR5 – BR-GBD (apo form of actin nucleation-promoting factor WASL)

Actin nucleation-promoting factor WASL experimental SAS data
Actin nucleation-promoting factor WASL Kratky plot
Sample: Actin nucleation-promoting factor WASL monomer, 19 kDa Rattus norvegicus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
...Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 3.0 nm
Dmax 9.9 nm
VolumePorod 19 nm3

SASDMS5 – Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion

RNA recognition motif (RRM)-containing protein 4 experimental SAS data
Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion Rg histogram
Sample: RNA recognition motif (RRM)-containing protein 4 monomer, 111 kDa Ustilago maydis protein
Buffer: 20 mM Hepes, 200 mM NaCl, 1 mM βME, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 30
A MademoiseLLE domain binding platform links the key RNA transporter to endosomes PLOS Genetics 18(6):e1010269 (2022)
...Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A
RgGuinier 8.8 nm
Dmax 30.7 nm
VolumePorod 587 nm3

SASDTS5 – Sorting nexin-9, N-terminus - SNX9-SH3 (apo form)

Sorting nexin-9 experimental SAS data
Sorting nexin-9 Kratky plot
Sample: Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
...Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 2.2 nm
Dmax 7.1 nm
VolumePorod 42 nm3

SASDMT5 – N-terminal histidine tagged RNA recognition motif (RRM)-containing protein 4 NT4

RNA recognition motif (RRM)-containing protein 4 NT4 experimental SAS data
N-terminal histidine tagged RNA recognition motif (RRM)-containing protein 4 NT4 Rg histogram
Sample: RNA recognition motif (RRM)-containing protein 4 NT4 monomer, 40 kDa Ustilago maydis protein
Buffer: 20 mM Hepes, 200 mM NaCl, 1 mM βME, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 30
A MademoiseLLE domain binding platform links the key RNA transporter to endosomes PLOS Genetics 18(6):e1010269 (2022)
...Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A
RgGuinier 5.6 nm
Dmax 18.5 nm
VolumePorod 123 nm3

SASDTT5 – SemDΔAPH bound to Sorting nexin-9, N-terminus - SNX9-SH3

Uncharacterized proteinSorting nexin-9 experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
...Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 94 nm3

SASDTU5 – SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL)

Uncharacterized proteinActin nucleation-promoting factor WASL experimental SAS data
SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL) Rg histogram
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Actin nucleation-promoting factor WASL monomer, 19 kDa Rattus norvegicus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
...Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 4.2 nm
Dmax 15.9 nm
VolumePorod 102 nm3

SASDS56 – Nucleotide binding domain, NBD (ABC transporter domain) from the RTX toxin ABC transporter RtxB

RTX toxin transporter experimental SAS data
GASBOR model
Sample: RTX toxin transporter monomer, 28 kDa Kingella kingae ATCC … protein
Buffer: 100 mM HEPES, 10 % glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
Type 1 secretion necessitates a tight interplay between all domains of the ABC transporter Scientific Reports 14(1) (2024)
...Reiners J, Spitz O, Hachani E, Smits S, Schmitt L
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 48 nm3

SASDSU6 – Palivizumab IgG

Palivizumab IgG experimental SAS data
CORAL model
Sample: Palivizumab IgG monomer, 145 kDa Commercial (Synagis) protein
Buffer: PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14
Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry :105270 (2023)
...Reiners J, Christmann A, Lang P, Smits S, Kolmar H, Scheller J
RgGuinier 5.0 nm
Dmax 16.8 nm
VolumePorod 252 nm3

SASDSV6 – Nanobody AIP1 VHH

Anti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data
CORAL model
Sample: Anti-Idiotypic-Palivizumab-Nanobody1 monomer, 20 kDa Lama glama protein
Buffer: PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14
Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry :105270 (2023)
...Reiners J, Christmann A, Lang P, Smits S, Kolmar H, Scheller J
RgGuinier 2.6 nm
Dmax 8.8 nm
VolumePorod 31 nm3

SASDSW6 – Palivizumab Nanobody AIP1VHH complex

Palivizumab IgGAnti-Idiotypic-Palivizumab-Nanobody1 experimental SAS data
CORAL model
Sample: Palivizumab IgG monomer, 145 kDa Commercial (Synagis) protein
Anti-Idiotypic-Palivizumab-Nanobody1 monomer, 20 kDa Lama glama protein
Buffer: PBS (137 mM NaCl, 2.7 mM KCl, 12 mM HPO4 2−/H2PO4 −), pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Sep 14
Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors Journal of Biological Chemistry :105270 (2023)
...Reiners J, Christmann A, Lang P, Smits S, Kolmar H, Scheller J
RgGuinier 6.0 nm
Dmax 20.4 nm

SASDS47 – 1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR)

1-deoxy-D-xylulose-5-phosphate reductoisomerase (G328C, K361E, S551G) experimental SAS data
1-Deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) Rg histogram
Sample: 1-deoxy-D-xylulose-5-phosphate reductoisomerase (G328C, K361E, S551G) dimer, 104 kDa Toxoplasma gondii (strain … protein
Buffer: 20 mM Tris/HCl, 150 mM NaCl, 40 mM MgCl2, 2% glycerol,, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Apr 25
1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) as target for anti Toxoplasma gondii agents: crystal structure, biochemical characterisation and biological evaluation of inhibitors Biochemical Journal (2024)
...Reiners J, Gertzen C, Applegate V, Abdullaziz M, Gottstein J, Degrandi D, Wesemann M, Kurz T, Smits S, Pfeffer K
RgGuinier 3.3 nm
Dmax 10.4 nm
VolumePorod 176 nm3

SASDM67 – Hemolysin A (HlyA) from Escherichia coli UTI89

Hemolysin, plasmid (Hemolysin A) experimental SAS data
GASBOR model
Sample: Hemolysin, plasmid (Hemolysin A) dimer, 220 kDa Escherichia coli UTI89 protein
Buffer: 100 mM HEPES pH 8.0, 250 mM NaCl, 10 mM CaCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 6
Identity Determinants of the Translocation Signal for a Type 1 Secretion System Frontiers in Physiology 12 (2022)
...Reiners J, Ma M, Luisi B, Smits S, Schmitt L
RgGuinier 6.7 nm
Dmax 25.3 nm
VolumePorod 346 nm3

SASDLK7 – Bartonella effector protein (Bep1), 15 °C

Bartonella effector protein (Bep) substrate of VirB T4SS experimental SAS data
GASBOR model
Sample: Bartonella effector protein (Bep) substrate of VirB T4SS monomer, 64 kDa Bartonella clarridgeiae (strain … protein
Buffer: 25 mM Hepes, 300 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2020 May 29
Structure and function of Bartonella effector protein 1: target and interdomain interactions University of Basel PhD thesis 15051 (2023)
...Reiners
RgGuinier 4.1 nm
Dmax 13.4 nm
VolumePorod 83 nm3

SASDLL7 – Bartonella effector protein (Bep1), 20 °C

Bartonella effector protein (Bep) substrate of VirB T4SS experimental SAS data
GASBOR model
Sample: Bartonella effector protein (Bep) substrate of VirB T4SS monomer, 64 kDa Bartonella clarridgeiae (strain … protein
Buffer: 25 mM Hepes, 300 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 26
Structure and function of Bartonella effector protein 1: target and interdomain interactions University of Basel PhD thesis 15051 (2023)
...Reiners
RgGuinier 3.8 nm
Dmax 13.4 nm
VolumePorod 87 nm3

SASDLM7 – Bartonella effector protein (Bep1), 35 °C

Bartonella effector protein (Bep) substrate of VirB T4SS experimental SAS data
GASBOR model
Sample: Bartonella effector protein (Bep) substrate of VirB T4SS monomer, 64 kDa Bartonella clarridgeiae (strain … protein
Buffer: 25 mM Hepes, 300 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 26
Structure and function of Bartonella effector protein 1: target and interdomain interactions University of Basel PhD thesis 15051 (2023)
...Reiners
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 108 nm3

SASDMT7 – Lanthionine synthetase C-like protein (MadC)

Lanthionine synthetase C-like protein experimental SAS data
GASBOR model
Sample: Lanthionine synthetase C-like protein monomer, 50 kDa Clostridium sp. Maddingley … protein
Buffer: 50 mM HEPES, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2019 Nov 28
The structure of MadC from Clostridium maddingley reveals new insights into class I lanthipeptide cyclases Frontiers in Microbiology 13 (2023)
...Reiners J, Kedrov A, Smits S, Schmitt L
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 78 nm3

SASDED9 – Relaxase domain of TraI

Relaxase (Tra_2) domain of TraI experimental SAS data
GASBOR model
Sample: Relaxase (Tra_2) domain of TraI monomer, 46 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 11
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
...Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 61 nm3

SASDEE9 – TraI_2_C domain of TraI

TraI_2_C domain of TraI experimental SAS data
GASBOR model
Sample: TraI_2_C domain of TraI monomer, 21 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 11
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
...Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 2.2 nm
Dmax 6.5 nm
VolumePorod 40 nm3

SASDEF9 – TraI of Neisseria gonorrhoeae

TraI experimental SAS data
GASBOR model
Sample: TraI monomer, 91 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 5
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
...Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 7.3 nm
Dmax 31.4 nm
VolumePorod 293 nm3

SASDLY9 – Mouse guanylate-binding protein 7 - mGBP7

Gbp6 protein (Gbp7 - Gbp6 protein Mus musculus) experimental SAS data
GASBOR model
Sample: Gbp6 protein (Gbp7 - Gbp6 protein Mus musculus) dimer, 148 kDa Mus musculus protein
Buffer: 50 mM Tris, 5 mM MgCl, 2 mM DTT, pH: 8
Experiment: SAXS data collected at Xenocs Xeuss 2.0 Q-Xoom, Center for Structural Studies, Heinrich-Heine-University on 2019 Oct 2
Integrative modeling of guanylate binding protein dimers Protein Science (2023)
...Reiners J, Degrandi D, Legewie L, Stühler K, Pfeffer K, Poschmann G, Smits S, Strodel B
RgGuinier 5.4 nm
Dmax 17.0 nm
VolumePorod 176 nm3