Small Angle Scattering Biological Data Bank SASBDB


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33 hits found for Ryzhykau

SASDPL2 – Fused complex of cytochrome P450 143 and ferredoxin Rv1786 (Cyp143 and rv1786)

His-tagged fused complex of cytochrome P450 143 and ferredoxin Rv1786 experimental SAS data

Fused complex of cytochrome P450 143 and ferredoxin Rv1786 (Cyp143 and rv1786) Rg histogram

Sample:	His-tagged fused complex of cytochrome P450 143 and ferredoxin Rv1786 monomer, 54 kDa Mycobacterium tuberculosis protein
Buffer:	300 mM NaCl, 50 mM Tris/TrisHCl, 10% glycerol, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 7

Structural insights into 3Fe–4S ferredoxins diversity in M. tuberculosis highlighted by a first redox complex with P450 Frontiers in Molecular Biosciences 9 (2023)
...Ryzhykau Y, Smolskaya S, Sushko T, Tsumoto K, Grabovec I, Kapranov I, Okhrimenko I, Marin E, Shevtsov M, Mishin A, Kovalev K, Kuklin A, Gordeliy V, Kaluzhskiy L, Gnedenko O, Yablokov E, Ivanov A, Bors...

R_g^Guinier	2.5	nm
D_max	9.0	nm
Volume^Porod	85	nm³

SASDTU4 – 24×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag

Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) experimental SAS data

Sample:	Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) 24-mer, 755 kDa Helicobacter pylori (strain … protein
Buffer:	25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 30

Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
...Ryzhykau Y, Vlasov A

R_g^Guinier	7.6	nm
D_max	25.0	nm
Volume^Porod	2010	nm³

SASDTV4 – 8×FerSUMO - Chimeric protein complex of ferritin from Helicobacter pylori fused to an N-terminal His6-SUMO protein tag

Sample:	Bacterial non-heme ferritin (N19Q, I59V, N-terminal His-SUMO fusion) octamer, 252 kDa Helicobacter pylori (strain … protein
Buffer:	25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Jul 17

Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
...Ryzhykau Y, Vlasov A

R_g^Guinier	5.4	nm
D_max	16.0	nm
Volume^Porod	618	nm³

SASDTW4 – Ferritin from Helicobacter pylori with an N-terminal His6-tag

Bacterial non-heme ferritin (N19Q, I59V) experimental SAS data

Sample:	Bacterial non-heme ferritin (N19Q, I59V) 24-mer, 480 kDa Helicobacter pylori (strain … protein
Buffer:	20 mM Tris, pH: 8
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Dec 14

Ferritin-based fusion protein shows octameric deadlock state of self-assembly Biochemical and Biophysical Research Communications 690:149276 (2024)
...Ryzhykau Y, Vlasov A

R_g^Guinier	5.6	nm
D_max	14.5	nm
Volume^Porod	674	nm³

SASDTX4 – G-actin - Skeletal muscle actin from Oryctolagus cuniculus: globular actin monomers

Actin, alpha skeletal muscle experimental SAS data

Sample:	Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer:	5 mM Tris/Tris-HCl, 0.1 mM CaCl2, 1 mM NaN3, 0.2 mM ATP, pH: 8.1
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 1

Small-angle X-ray scattering structural insights into alternative pathway of actin oligomerization associated with inactivated state Biochemical and Biophysical Research Communications :149340 (2023)
Ryzhykau Y, Povarova O, Dronova E, Kuklina D, Antifeeva I, Ilyinsky N, Okhrimenko I, Semenov Y, Kuklin A, Ivanovich V, Fonin A, Uversky V, Turoverov K, Kuznetsova I

R_g^Guinier	2.9	nm
D_max	8.5	nm
Volume^Porod	58	nm³

SASDTY4 – F-actin - Skeletal muscle actin from Oryctolagus cuniculus: fibrillar actin

Sample:	Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer:	5 mM Tris/Tris-HCl, 0.1 mM CaCl2, 1 mM NaN3, 1.0 mM ATP, 50 mM KCl, 2 mM MgCl2, pH: 8.1
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 1

R_g^Guinier	15.7	nm
D_max	60.0	nm
Volume^Porod	4710	nm³

SASDTZ4 – I-actin - Skeletal muscle actin from Oryctolagus cuniculus: oligomer of time-inactivated actin (peak SEC fraction)

Sample:	Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer:	5 mM Tris/Tris-HCl, 2.0 mM EDTA, pH: 8.1
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Nov 16

R_g^Guinier	4.9	nm
D_max	22.0	nm
Volume^Porod	549	nm³

SASDT25 – G- / I-actin mixture - Skeletal muscle actin from Oryctolagus cuniculus: mixture of globular actin monomers and inactivated actin monomers and dimers

Sample:	Actin, alpha skeletal muscle monomer, 42 kDa Oryctolagus cuniculus protein
Buffer:	5 mM Tris, 0.1 mM CaCl2, 1 mM NaN3, 0.2 mM ATP, pH: 8.1
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2021 Jul 5

R_g^Guinier	3.1	nm
D_max	12.0	nm
Volume^Porod	84	nm³

SASDQA7 – Homodimeric luciferase EsLuxA

Alkanal monooxygenase alpha chain experimental SAS data

Sample:	Alkanal monooxygenase alpha chain dimer, 84 kDa Enhygromyxa salina protein
Buffer:	150 mM NaCl, 25 mM Tris, pH: 8
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Aug 1

luxA Gene From Enhygromyxa salina Encodes a Functional Homodimeric Luciferase Proteins: Structure, Function, and Bioinformatics (2024)
...Ryzhykau Y, Semenov Y, Kuklin A, Manukhov I, Gushchin I

R_g^Guinier	3.3	nm
D_max	12.5	nm
Volume^Porod	138	nm³

SASDRR8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea purified by anion-exchange chromatography (~ 300 mM NaCl)

ATP synthase subunit alpha, chloroplasticATP synthase subunit beta, chloroplasticATP synthase gamma chain, chloroplasticATP synthase delta chain, chloroplasticATP synthase epsilon chain, chloroplasticATP synthase subunit a, chloroplasticATP synthase subunit b, chloroplasticATP synthase subunit b', chloroplasticATP synthase subunit c, chloroplastic4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside experimental SAS data

Sample:	ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa
Buffer:	300 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3

I-Shaped Dimers of a Plant Chloroplast FOF1-ATP Synthase in Response to Changes in Ionic Strength International Journal of Molecular Sciences 24(13):10720 (2023)
...Ryzhykau Y, Zinovev E, Minaeva A, Ivashchenko S, Verteletskiy D, Sudarev V, Kuklina D, Nikolaev M, Semenov Y, Zagryadskaya Y, Okhrimenko I, Gette M, Dronova E, Shishkin A, Dencher N, Kuklin A, Ivanovi...

R_g^Guinier	6.6	nm
D_max	27.5	nm
Volume^Porod	927	nm³

SASDRS8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 150 mM NaCl

Sample:	ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa
Buffer:	150 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3

R_g^Guinier	9.6	nm
D_max	41.5	nm
Volume^Porod	1506	nm³

SASDRT8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 250 mM NaCl

Sample:	ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa
Buffer:	250 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3

R_g^Guinier	7.4	nm
D_max	33.0	nm
Volume^Porod	949	nm³

SASDRU8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 300 mM NaCl

Sample:	ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa
Buffer:	300 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3

R_g^Guinier	7.8	nm
D_max	39.5	nm
Volume^Porod	1127	nm³

SASDRV8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 350 mM NaCl

Sample:	ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa
Buffer:	350 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3

R_g^Guinier	8.9	nm
D_max	44.5	nm
Volume^Porod	1150	nm³

SASDRW8 – Сhloroplast FOF1-ATP synthase from Spinacia oleracea at 450 mM NaCl

Sample:	ATP synthase subunit alpha, chloroplastic trimer, 166 kDa Spinacia oleracea protein ATP synthase subunit beta, chloroplastic trimer, 161 kDa Spinacia oleracea protein ATP synthase gamma chain, chloroplastic monomer, 40 kDa Spinacia oleracea protein ATP synthase delta chain, chloroplastic monomer, 28 kDa Spinacia oleracea protein ATP synthase epsilon chain, chloroplastic monomer, 15 kDa Spinacia oleracea protein ATP synthase subunit a, chloroplastic monomer, 27 kDa Spinacia oleracea protein ATP synthase subunit b, chloroplastic monomer, 21 kDa Spinacia oleracea protein ATP synthase subunit b', chloroplastic monomer, 24 kDa Spinacia oleracea protein ATP synthase subunit c, chloroplastic 14-mer, 112 kDa Spinacia oleracea protein 4-trans-(4-trans-Propylcyclohexyl)-cyclohexyl α-maltoside 0, 283 kDa
Buffer:	450 mM NaCl, 30 mM HEPES, 2 mM MgCl2, 0.04% (w/v) tPCC-α-M, pH: 8
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2020 Oct 3

R_g^Guinier	10.8	nm
D_max	46.5	nm
Volume^Porod	1703	nm³

SASDJ69 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 150 mM NaCl studied with SAXS

Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 8

Ambiguities in and completeness of SAS data analysis of membrane proteins: the case of the sensory rhodopsin II–transducer complex Acta Crystallographica Section D Structural Biology 77(11) (2021)
Ryzhykau Y, Vlasov A, Orekhov P, Rulev M, Rogachev A, Vlasova A, Kazantsev A, Verteletskiy D, Skoi V, Brennich M, Pernot P, Murugova T, Gordeliy V, Kuklin A

R_g^Guinier	7.6	nm
D_max	41.0	nm

SASDJ79 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 150 mM NaCl studied with SANS

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25

R_g^Guinier	8.9	nm
D_max	39.0	nm

SASDJ89 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 1400 mM NaCl studied with SANS

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	1400 mM NaCl, 49.4 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Feb 10

R_g^Guinier	8.6	nm
D_max	39.0	nm

SASDJ99 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 2800 mM NaCl studied with SANS

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	2800 mM NaCl, 76.6 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Feb 10

R_g^Guinier	9.0	nm
D_max	39.0	nm

SASDJA9 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 4000 mM NaCl studied with SANS

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	4000 mM NaCl, 100 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25

R_g^Guinier	10.4	nm
D_max	39.0	nm

SASDPA4 – SAXS data for the trimeric C-phycocyanin

C-phycocyanin alpha subunitC-phycocyanin beta subunit experimental SAS data

Sample:	C-phycocyanin alpha subunit trimer, 53 kDa Arthrospira platensis protein C-phycocyanin beta subunit trimer, 54 kDa Arthrospira platensis protein
Buffer:	150 mM NaCl, 20 mM Tris, pH: 7.6
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Apr 14

Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores Structural Dynamics 9(5):054701 (2022)
...Ryzhykau Y, Kuklin A, Semenov Y, Ge B, Li W, Qin S, Friedrich T, Sluchanko N, Maksimov E

R_g^Guinier	3.9	nm
D_max	13.2	nm
Volume^Porod	154	nm³

SASDPQ5 – His-tagged L-methionine gamma-lyase from Clostridium tetani

L-methionine gamma-lyase experimental SAS data

Sample:	L-methionine gamma-lyase tetramer, 181 kDa Clostridium tetani protein
Buffer:	PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19

Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding EGFR Biochemical and Biophysical Research Communications :149319 (2023)
...Ryzhykau Y, Okhrimenko I, Zagryadskaya Y, Maslov I, Anisimova N, Sokolova D, Kuklin A, Pokrovsky V, Manukhov I

R_g^Guinier	4.0	nm
D_max	14.9	nm
Volume^Porod	232	nm³

SASDKZ6 – SAXS data for the complex of the sensory rhodopsin II with its cognate truncated transducer in detergent at 0.15 M NaCl

Sensory rhodopsin II from Natronbacterium pharaonisTruncated sensory rhodopsin II transducer containing transmembrane and HAMP1 domains experimental SAS data

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Truncated sensory rhodopsin II transducer containing transmembrane and HAMP1 domains dimer, 30 kDa Natronomonas pharaonis protein
Buffer:	150 mM NaCl, 25 Na/K-Pi, 1 mM EDTA, 0.05 % DDM, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Dec 13

Molecular model of a sensor of two-component signaling system Scientific Reports 11(1) (2021)
Ryzhykau Y, Orekhov P, Rulev M, Vlasov A, Melnikov I, Volkov D, Nikolaev M, Zabelskii D, Murugova T, Chupin V, Rogachev A, Gruzinov A, Svergun D, Brennich M, Gushchin I, Soler-Lopez M, Bothe A, Büldt ...

R_g^Guinier	4.8	nm
D_max	14.0	nm

SASDJY7 – A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH)

Modular nanotransporter with a melanocyte stimulating hormone ligand module experimental SAS data

A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH) Rg histogram

Sample:	Modular nanotransporter with a melanocyte stimulating hormone ligand module monomer, 70 kDa synthetic construct protein
Buffer:	150 mM NaCl, 10 mM phosphate buffer saline, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2016 Nov 24

Low-resolution structures of modular nanotransporters shed light on their functional activity Acta Crystallographica Section D Structural Biology 76(12):1270-1279 (2020)
...Ryzhykau Y, Kuklin A, Orekhov A, Eydlin I, Georgiev G, Gordeliy V, Sobolev A

R_g^Guinier	3.7	nm
D_max	13.3	nm
Volume^Porod	127	nm³

SASDPB4 – SAXS data for the C-phycocyanin in 8 M urea solution

Sample:	C-phycocyanin alpha subunit monomer, 18 kDa Arthrospira platensis protein C-phycocyanin beta subunit monomer, 18 kDa Arthrospira platensis protein
Buffer:	8 M urea, 30 mM βME, pH: 7
Experiment:	SAXS data collected at Rigaku MicroMax 007-HF, Moscow Institute of Physics and Technology (MIPT) on 2022 Apr 14

R_g^Guinier	6.6	nm
D_max	23.5	nm

SASDPS5 – L-methionine gamma-lyase from Clostridium sporogenes fused with S3 domain of the Vaccinia virus growth factor

L-methionine gamma-lyase from Clostridium sporogenes fused with VGF S3 domain experimental SAS data

Sample:	L-methionine gamma-lyase from Clostridium sporogenes fused with VGF S3 domain tetramer, 183 kDa Clostridium sporogenes protein
Buffer:	PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19

R_g^Guinier	5.2	nm
D_max	17.3	nm
Volume^Porod	303	nm³

SASDK27 – SAXS data for the sensory rhodopsin II / transducer complex in detergent at 0.15 M NaCl

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 9

R_g^Guinier	9.0	nm
D_max	38.9	nm

SASDJZ7 – A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to an epidermal growth factor ligand module (MNT-EGF)

Modular nanotransporter with an epidermal growth factor ligand module experimental SAS data

Sample:	Modular nanotransporter with an epidermal growth factor ligand module monomer, 76 kDa synthetic construct protein
Buffer:	150 mM NaCl, 10 mM phosphate buffer saline, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2016 Nov 24

R_g^Guinier	4.2	nm
D_max	16.0	nm
Volume^Porod	161	nm³

SASDPR5 – L-methionine gamma-lyase from Clostridium sporogenes

L-methionine gamma-lyase (K272S) experimental SAS data

Sample:	L-methionine gamma-lyase (K272S) tetramer, 174 kDa Clostridium sporogenes protein
Buffer:	PBS-D2O: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4 (D2O buffer), pH: 7.4
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 May 19

R_g^Guinier	3.7	nm
D_max	14.5	nm
Volume^Porod	211	nm³

SASDK37 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 0.15 M NaCl

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	150 mM NaCl, 25 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25

R_g^Guinier	7.1	nm
D_max	35.0	nm

SASDK47 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 1.4 M NaCl

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	1400 mM NaCl, 49.4 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Feb 10

R_g^Guinier	9.9	nm
D_max	36.5	nm

SASDK57 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 2.8 M NaCl

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	2800 mM NaCl, 76.6 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Feb 10

R_g^Guinier	9.3	nm
D_max	37.5	nm

SASDK67 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 4.0 M NaCl

Sample:	Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer:	4000 mM NaCl, 100 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment:	SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25

R_g^Guinier	8.9	nm
D_max	39.0	nm

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