Small Angle Scattering Biological Data Bank SASBDB


Advanced search

11 hits found for Soni

SASDM43 – RRM1-ZnF1 tandem domains of RNA-binding protein 5 (C191G mutant)

RNA-binding protein 5 (I107T, C191G) experimental SAS data

Sample:	RNA-binding protein 5 (I107T, C191G) monomer, 14 kDa Homo sapiens protein
Buffer:	20 mM MES, 400 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Sep 26

Structural basis for specific RNA recognition by the alternative splicing factor RBM5. Nat Commun 14(1):4233 (2023)
Soni K, Jagtap PKA, Martínez-Lumbreras S, Bonnal S, Geerlof A, Stehle R, Simon B, Valcárcel J, Sattler M

R_g^Guinier	1.7	nm
D_max	5.6	nm
Volume^Porod	28	nm³

SASDM53 – RRM1-ZnF1-RRM2 triple domains of RNA-binding protein 5 (C191G mutant)

RNA Binding Motif protein 5 (I107T, C191G) experimental SAS data

RNA Binding Motif protein 5 (I107T, C191G) Kratky plot

Sample:	RNA Binding Motif protein 5 (I107T, C191G) monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM MES, 400 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2017 Feb 9

R_g^Guinier	2.3	nm
D_max	7.8	nm
Volume^Porod	36	nm³

SASDM63 – RRM1-ZnF1-RRM2 triple domains of RNA-binding protein 5 (C191G mutant) in complex with GGCU_12 RNA

RNA Binding Motif protein 5 (I107T, C191G)Caspase-2 derived RNA GGCU_12 experimental SAS data

Sample:	RNA Binding Motif protein 5 (I107T, C191G) monomer, 26 kDa Homo sapiens protein Caspase-2 derived RNA GGCU_12 monomer, 4 kDa RNA
Buffer:	20 mM MES, 100 mM NaCl, 1 mM DTT, pH: 6.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2017 Mar 1

R_g^Guinier	2.2	nm
D_max	7.5	nm
Volume^Porod	42	nm³

SASDK24 – Signal recognition particle SRP9/14 heterodimer from Plasmodium falciparum

Signal recognition particle 9Signal recognition particle 14 experimental SAS data

Sample:	Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Feb 22

Structural analysis of the SRP Alu domain from Plasmodium falciparum reveals a non-canonical open conformation. Commun Biol 4(1):600 (2021)
Soni K, Kempf G, Manalastas-Cantos K, Hendricks A, Flemming D, Guizetti J, Simon B, Frischknecht F, Svergun DI, Wild K, Sinning I

R_g^Guinier	2.1	nm
D_max	7.2	nm
Volume^Porod	47	nm³

SASDK34 – Full length SRP Alu RNA from Plasmodium falciparum

Full-length SRP Alu RNA experimental SAS data

Sample:	Full-length SRP Alu RNA monomer, 38 kDa Plasmodium falciparum RNA
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 22

R_g^Guinier	3.3	nm
D_max	11.8	nm
Volume^Porod	63	nm³

SASDK44 – The 5' domain of SRP Alu RNA from Plasmodium falciparum

SRP Alu RNA 5' domain experimental SAS data

Sample:	SRP Alu RNA 5' domain monomer, 24 kDa Plasmodium falciparum RNA
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 22

R_g^Guinier	3.3	nm
D_max	11.5	nm
Volume^Porod	38	nm³

SASDK54 – Signal recognition particle SRP9/14 heterodimer in complex with full length SRP Alu RNA from Plasmodium falciparum

Signal recognition particle 9Signal recognition particle 14Full-length SRP Alu RNA experimental SAS data

Sample:	Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein Full-length SRP Alu RNA monomer, 38 kDa Plasmodium falciparum RNA
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 22

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	120	nm³

SASDK64 – Signal recognition particle SRP9/14 heterodimer in complex with the 5' domain of SRP Alu RNA from Plasmodium falciparum

Signal recognition particle 9Signal recognition particle 14SRP Alu RNA 5' domain experimental SAS data

Sample:	Signal recognition particle 9 monomer, 12 kDa Plasmodium falciparum protein Signal recognition particle 14 monomer, 12 kDa Plasmodium falciparum protein SRP Alu RNA 5' domain monomer, 24 kDa Plasmodium falciparum RNA
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 10 mM MgCl2, 10 mM KCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 22

R_g^Guinier	3.2	nm
D_max	11.9	nm
Volume^Porod	77	nm³

SASDHJ8 – ACT domain of the Mycobacterium tuberculosis Rel protein

ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) experimental SAS data

ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) Kratky plot

Sample:	ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) dimer, 20 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 350 mM NaCl, 5% glycerol, 1 mM DTT, pH: 8.5
Experiment:	SAXS data collected at Bruker Nanostar, Nanyang Technological University on 2018 Jun 7

Atomic structure of, and valine binding to the regulatory ACT domain of the Mycobacterium tuberculosis Rel protein. FEBS J (2020)
Shin J, Singal B, Manimekalai MSS, Chen MW, Ragunathan P, Grüber G

R_g^Guinier	1.9	nm
D_max	6.1	nm
Volume^Porod	29	nm³

SASDKE6 – Poly(A) polymerase pla1 bound to the NURS complex subunit red1

NURS complex subunit red1Poly(A) polymerase pla1 experimental SAS data

Sample:	NURS complex subunit red1 monomer, 8 kDa Schizosaccharomyces pombe (strain … protein Poly(A) polymerase pla1 monomer, 65 kDa Schizosaccharomyces pombe (strain … protein
Buffer:	20 mM HEPES, pH 7.5, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12

Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex. Nat Commun 14(1):772 (2023)
Soni K, Sivadas A, Horvath A, Dobrev N, Hayashi R, Kiss L, Simon B, Wild K, Sinning I, Fischer T

R_g^Guinier	3.3	nm
D_max	10.7	nm
Volume^Porod	138	nm³

SASDKD6 – NURS complex subunit red1

NURS complex subunit red1 experimental SAS data

Sample:	NURS complex subunit red1 monomer, 8 kDa Schizosaccharomyces pombe (strain … protein
Buffer:	20 mM HEPES, pH 7.5, 150 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12

R_g^Guinier	2.5	nm
D_max	8.7	nm
Volume^Porod	12	nm³

Search