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37 hits found for Soumya G Remesh

SASDDT4 – ...gion of Immunoglobulin G1 (IgG1 Fc)

Immunoglobulin heavy constant gamma 1 experimental SAS data
BILBOMD model
Sample: ...globulin heavy constant gamma 1 dimer, 53 kDa Homo sapiens protein
Buffer: 20mM HEPES, 50mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2016 Feb 17
...globulin Fc Domain in Solution. Structure 26(7):1007-1014.e2 (2018)
Remesh SG, Armstrong AA, Mahan AD, Luo J, Hammel M
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 70 nm3

SASDDU4 – ...gion of Immunoglobulin G2 (IgG2 Fc)

Immunoglobulin heavy constant gamma 2 experimental SAS data
BILBOMD model
Sample: ...globulin heavy constant gamma 2 dimer, 52 kDa Homo sapiens protein
Buffer: 20mM HEPES, 50mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2016 Feb 17
...globulin Fc Domain in Solution. Structure 26(7):1007-1014.e2 (2018)
Remesh SG, Armstrong AA, Mahan AD, Luo J, Hammel M
RgGuinier 2.8 nm
Dmax 9.0 nm
VolumePorod 67 nm3

SASDDV4 – ...gion of Immunoglobulin G1, M135Y/S137T/T139E mutant (IgG1 Fc-YTE)

Immunoglobulin heavy constant gamma 1 M255Y/S257T/T259E experimental SAS data
BILBOMD model
Sample: ...globulin heavy constant gamma 1 M255Y/S257T/T259E dimer, 53 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 50mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2016 Feb 17
...globulin Fc Domain in Solution. Structure 26(7):1007-1014.e2 (2018)
Remesh SG, Armstrong AA, Mahan AD, Luo J, Hammel M
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 74 nm3

SASDFV6 – ...g protein HU-alpha, E38K/V42L double mutant

DNA-binding protein HU-alpha, E38K/V42L double mutant experimental SAS data
CHIMERA model
Sample: ...g protein HU-alpha, E38K/V42L double mutant decamer, 95 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2015 Apr 23
...g during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 53 nm3

SASDFW6 – ...g protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:2)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E38K/V42L double mutant experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E38K/V42L double mutant tetramer, 38 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2015 Apr 23
...g during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh
RgGuinier 5.7 nm
Dmax 31.3 nm
VolumePorod 297 nm3

SASDFX6 – ...g protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:4)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E38K/V42L double mutant experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E38K/V42L double mutant tetramer, 38 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2015 Apr 23
...g during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh
RgGuinier 5.7 nm
Dmax 25.7 nm
VolumePorod 195 nm3

SASDFY6 – ...g protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:8)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E38K/V42L double mutant experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E38K/V42L double mutant octamer, 76 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2015 Apr 23
...g during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh
RgGuinier 5.8 nm
Dmax 28.1 nm
VolumePorod 296 nm3

SASDFZ6 – ...g protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:16)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E38K/V42L double mutant experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E38K/V42L double mutant 16-mer, 153 kDa Linked to wild-type … protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2015 Apr 23
...g during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh
RgGuinier 6.3 nm
Dmax 27.3 nm
VolumePorod 401 nm3

SASDKJ8 – ...g protein 1 (CDCP1)

CUB domain-containing protein 1 experimental SAS data
CUB domain-containing protein 1 Kratky plot
Sample: ...g protein 1 monomer, 77 kDa Homo sapiens protein
Buffer: 10 mM phosphate, 137 mM NaCl, 2.7 mM KCl,, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2020 Jul 29
...geting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers. J Clin Invest 132(4) (2022)
...g YH, Filippova EV, Steri V, Wang D, Remesh SG, Liu J, Hann B, Kossiakoff AA, Evans MJ, Leung KK, Wells JA
RgGuinier 4.4 nm
Dmax 14.5 nm
VolumePorod 185 nm3

SASDKK8 – ...g protein 1 (CDCP1)

Cleaved - CUB domain-containing protein 1 (N-terminus)Cleaved - CUB domain-containing protein 1 (C-terminus) experimental SAS data
Cleaved - CUB domain-containing protein 1 (N-terminus) Cleaved - CUB domain-containing protein 1 (C-terminus) Kratky plot
Sample: ...g protein 1 (N-terminus) monomer, 38 kDa Homo sapiens protein
...g protein 1 (C-terminus) monomer, 39 kDa Homo sapiens protein
Buffer: 10 mM phosphate, 137 mM NaCl, 2.7 mM KCl,, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2020 Jul 29
...geting a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) for RAS-driven cancers. J Clin Invest 132(4) (2022)
...g YH, Filippova EV, Steri V, Wang D, Remesh SG, Liu J, Hann B, Kossiakoff AA, Evans MJ, Leung KK, Wells JA
RgGuinier 4.4 nm
Dmax 14.3 nm
VolumePorod 182 nm3

SASDFR6 – ...g protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:1)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E34K , 228 kDa Escherichia coli protein
Buffer: 20mM HEPES, 100mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2016 Jul 8
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 6.1 nm
Dmax 21.9 nm
VolumePorod 251 nm3

SASDFS6 – ...g protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:2.5)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E34K , 267 kDa Escherichia coli protein
Buffer: 20mM HEPES, 100mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2016 Jul 8
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 7.0 nm
Dmax 25.0 nm
VolumePorod 386 nm3

SASDFT6 – ...g protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:5)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E34K , 267 kDa Escherichia coli protein
Buffer: 20mM HEPES, 100mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2016 Jul 8
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 7.2 nm
Dmax 27.0 nm
VolumePorod 474 nm3

SASDFU6 – ...g protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:10)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E34K , 305 kDa Escherichia coli protein
Buffer: 20mM HEPES, 100mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2016 Jul 8
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 8.0 nm
Dmax 24.8 nm
VolumePorod 448 nm3

SASDFN6 – ...g protein HU-alpha

DNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: ...g protein HU-alpha octamer, 77 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 May 27
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 3.2 nm
Dmax 10.7 nm

SASDFP6 – 80 base pair DNA

80bp_DNA Forward80bp_DNA Reverse experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
Buffer: 10 mM Bis-Tris, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 7.0 nm
Dmax 28.9 nm

SASDF36 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 50 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha , 10 kDa Escherichia coli protein
Buffer: 10 mM sodium acetate, 50 mM NaCl, pH: 4.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 May 27
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDF46 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 100 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha , 10 kDa Escherichia coli protein
Buffer: 10 mM sodium acetate, 100 mM NaCl, pH: 4.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDF56 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 150 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha , 10 kDa Escherichia coli protein
Buffer: 10 mM sodium acetate, 150 mM NaCl, pH: 4.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDF66 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 4.5 with 300 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha , 10 kDa Escherichia coli protein
Buffer: 10 mM sodium acetate, 300 mM NaCl, pH: 4.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDFX5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 5.5 with 50 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha , 10 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 50 mM NaCl, pH: 5.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 May 27
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDFY5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 5.5 with 100 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha , 10 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 100 mM NaCl, pH: 5.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 May 27
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDFZ5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 5.5 with 150 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha , 10 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 May 27
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDF26 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 5.5 with 300 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha decamer, 95 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 300 mM NaCl, pH: 5.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 May 27
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 6.4 nm
Dmax 24.4 nm
VolumePorod 268 nm3

SASDFT5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 6.5 with 50 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha , 10 kDa Escherichia coli protein
Buffer: 10mM Bis-Tris, 50 mM NaCl, pH: 6.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 May 27
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDFU5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 6.5 with 100 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha 14-mer, 133 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 6.2 nm
Dmax 24.4 nm
VolumePorod 274 nm3

SASDFV5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 6.5 with 150 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha 14-mer, 133 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 7.0 nm
Dmax 26.2 nm
VolumePorod 352 nm3

SASDFW5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 6.5 with 300 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha decamer, 95 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 300 mM NaCl, pH: 6.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 6.0 nm
Dmax 24.7 nm
VolumePorod 218 nm3

SASDFP5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 7.5 with 50 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha 16-mer, 153 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 May 27
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 8.9 nm
Dmax 28.5 nm
VolumePorod 410 nm3

SASDFQ5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 7.5 with 100 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha 16-mer, 153 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 6.6 nm
Dmax 25.0 nm
VolumePorod 336 nm3

SASDFR5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 7.5 with 150 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha 14-mer, 133 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 5.8 nm
Dmax 24.2 nm
VolumePorod 308 nm3

SASDFS5 – ...g protein HU-alpha bound to 80 base-pair DNA at pH 7.5 with 300 mM NaCl

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha experimental SAS data
CHIMERA model
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha decamer, 95 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 6.5 nm
Dmax 24.0 nm
VolumePorod 242 nm3

SASDFQ6 – ...g protein HU-alpha, E34K

DNA-binding protein HU-alpha, E34K experimental SAS data
CHIMERA model
Sample: ...g protein HU-alpha, E34K dimer, 19 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Jun 1
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 2.2 nm
Dmax 6.7 nm
VolumePorod 36 nm3

...GB3 – ...g protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha, E34K Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E34K dimer, 19 kDa Escherichia coli protein
Buffer: 10 mM sodium acetate, 50 mM NaCl, pH: 4.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Nov 2
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

...GC3 – ...g protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 5.5)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha, E34K Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E34K dimer, 19 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 50 mM NaCl, pH: 5.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Nov 2
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

...GD3 – ...g protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 6.5)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha, E34K Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E34K , 100 kDa Escherichia coli protein
Buffer: 10mM Bis-Tris, 50 mM NaCl, pH: 6.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Nov 2
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 7.3 nm
Dmax 28.0 nm
VolumePorod 330 nm3

...GE3 – ...g protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 7.5)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data
80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha, E34K Kratky plot
Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA
80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA
...g protein HU-alpha, E34K , 100 kDa Escherichia coli protein
Buffer: 10 mM Bis-Tris, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...ght Source (ALS) on 2018 Nov 2
...g during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
RgGuinier 7.1 nm
Dmax 27.5 nm
VolumePorod 309 nm3