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44 hits found for Thureau

SASDBT2 – Ankyrin repeat domains from human Tankyrase-2 (489-649)

Ankyrin repeat domains from Tankyrase 2 experimental SAS data
Ankyrin repeat domains from Tankyrase 2 Kratky plot
Sample: Ankyrin repeat domains from Tankyrase 2 monomer, 18 kDa Homo sapiens protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
...Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 1.8 nm
Dmax 6.3 nm
VolumePorod 24 nm3

SASDBU2 – Human Arpin (isoform 1)

Human Arpin experimental SAS data
Human Arpin Kratky plot
Sample: Human Arpin monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
...Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 2.6 nm
Dmax 13.2 nm
VolumePorod 47 nm3

SASDBV2 – Zebrafish Arpin

Zebrafish (Danio rerio) Arpin experimental SAS data
Zebrafish (Danio rerio) Arpin Kratky plot
Sample: Zebrafish (Danio rerio) Arpin monomer, 25 kDa Danio rerio protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
...Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 2.7 nm
Dmax 13.8 nm
VolumePorod 47 nm3

SASDBW2 – Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16).

Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). experimental SAS data
Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). Kratky plot
Sample: Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). monomer, 24 kDa Danio rerio protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
...Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 2.2 nm
Dmax 11.3 nm
VolumePorod 37 nm3

SASDBX2 – Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)

Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex experimental SAS data
Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex Kratky plot
Sample: Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex monomer, 43 kDa Danio rerio / … protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
...Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 3.3 nm
Dmax 12.6 nm
VolumePorod 58 nm3

SASDPH3 – Benchmarking extraction methods of scattering patterns of poorly resolved components from a SEC-SAXS dataset of BSA.

Bovine Serum Albumin experimental SAS data
Bovine Serum Albumin Kratky plot
Sample: Bovine Serum Albumin trimer, 199 kDa Bos taurus protein
Buffer: 20 mM Hepes, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Sep 24
SEC-SAXS: Experimental set-up and software developments build up a powerful tool. Methods Enzymol 677:221-249 (2022)
...Thureau A, Vachette P
RgGuinier 5.2 nm
Dmax 17.6 nm
VolumePorod 370 nm3

SASDPP4 – Consensus SAXS Profile - Ribonuclease A

Ribonuclease pancreatic experimental SAS data
DAMMIN model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.5 nm
Dmax 4.9 nm
VolumePorod 18 nm3

SASDPQ4 – Consensus SAXS Profile - Urate Oxidase

Uricase experimental SAS data
DAMMIN model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 3.2 nm
Dmax 9.2 nm
VolumePorod 220 nm3

SASDPR4 – Consensus SAXS Profile - Xylose Isomerase

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: ConsensusBuffer_50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 3.3 nm
Dmax 10.1 nm
VolumePorod 243 nm3

SASDPS4 – Consensus SAXS Profile - Xylanase

Endo-1,4-beta-xylanase experimental SAS data
DAMMIN model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.6 nm
Dmax 5.1 nm
VolumePorod 27 nm3

SASDPT4 – Consensus SAXS Profile - Lysozyme

Lysozyme C experimental SAS data
DAMMIN model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 19 nm3

SASDPU4 – Consensus SANS Profile - Ribonuclease A in 100% v/v D2O buffer

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.4 nm
Dmax 4.4 nm

SASDGV4 – Model of the RH1-LZ1 domains of C-Jun-amino-terminal kinase-interacting protein 3 (JIP3)

C-Jun-amino-terminal kinase-interacting protein 3 experimental SAS data
OTHER model
Sample: C-Jun-amino-terminal kinase-interacting protein 3 dimer, 40 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 0.5 mM TCEP, pH: 7.1
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Sep 22
Structural characterization of the RH1-LZI tandem of JIP3/4 highlights RH1 domains as a cytoskeletal motor-binding motif. Sci Rep 9(1):16036 (2019)
...Thureau A, Pylypenko O, Andreani J, Llinas P, Ménétrey J
RgGuinier 6.3 nm
Dmax 23.1 nm
VolumePorod 140 nm3

SASDPV4 – Consensus SANS Profile - Lysozyme in 100% v/v D2O buffer

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.2 nm
Dmax 3.8 nm

SASDGW4 – Model of the LZ1 domain of C-Jun-amino-terminal kinase-interacting protein 3 (JIP3)

C-Jun-amino-terminal kinase-interacting protein 3 experimental SAS data
OTHER model
Sample: C-Jun-amino-terminal kinase-interacting protein 3 dimer, 40 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, 0.5 mM TCEP, pH: 7.1
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Sep 22
Structural characterization of the RH1-LZI tandem of JIP3/4 highlights RH1 domains as a cytoskeletal motor-binding motif. Sci Rep 9(1):16036 (2019)
...Thureau A, Pylypenko O, Andreani J, Llinas P, Ménétrey J
RgGuinier 5.0 nm
Dmax 19.8 nm
VolumePorod 84 nm3

SASDPW4 – Consensus SANS Profile - Xylanase in 100% v/v D2O buffer

Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.5 nm
Dmax 4.4 nm

SASDPX4 – Consensus SANS Profile - Urate oxidase in 100% v/v D2O buffer

Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 3.1 nm
Dmax 9.3 nm

SASDPY4 – Consensus SANS Profile - Xylose isomerase in 100% v/v D2O buffer

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 3.1 nm
Dmax 9.5 nm

SASDPZ4 – Consensus SANS Profile - Lysozyme in H2O buffer

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.4 nm
Dmax 4.8 nm

SASDP25 – Consensus SANS Profile - Ribonuclease A in H2O buffer

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.5 nm
Dmax 4.1 nm

SASDP35 – Consensus SANS Profile - Xylanase in H2O buffer

Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 1.6 nm
Dmax 4.3 nm

SASDP45 – Consensus SANS Profile - Urate oxidase in H2O buffer

Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 3.2 nm
Dmax 9.1 nm

SASDP55 – Consensus SANS Profile - Xylose isomerase in H2O buffer

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Thureau A, Weiss T, Whitten A, Wood K, Zuo X
RgGuinier 3.3 nm
Dmax 9.7 nm

SASDNM7 – Protease 1 from Pyrococcus horikoshii (PhP1) in Gd-HPDO3A

Deglycase PH1704 experimental SAS data
OTHER model
Sample: Deglycase PH1704 hexamer, 112 kDa Pyrococcus horikoshii (strain … protein
Buffer: 20 mM Tris pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Jun 7
Medical contrast agents as promising tools for biomacromolecular SAXS experiments. Acta Crystallogr D Struct Biol 78(Pt 9):1120-1130 (2022)
...Thureau A, Mechulam Y, Pérez J, Girard E
RgGuinier 3.2 nm
Dmax 9.2 nm
VolumePorod 140 nm3

SASDNN7 – Protease 1 from Pyrococcus horikoshii (PhP1) in iohexol

Deglycase PH1704 experimental SAS data
OTHER model
Sample: Deglycase PH1704 hexamer, 112 kDa Pyrococcus horikoshii (strain … protein
Buffer: 20 mM Tris pH 7.5 and 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Nov 26
Medical contrast agents as promising tools for biomacromolecular SAXS experiments. Acta Crystallogr D Struct Biol 78(Pt 9):1120-1130 (2022)
...Thureau A, Mechulam Y, Pérez J, Girard E
RgGuinier 3.2 nm
Dmax 9.2 nm
VolumePorod 140 nm3

SASDNP7 – Translation initiation factor 2 (aIF2)-tRNA complex in Gd-HPDO3A

Translation initiation factor 2 subunit gammaTranslation initiation factor 2 subunit alphatransfer RNA experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Translation initiation factor 2 subunit gamma monomer, 46 kDa Saccharolobus solfataricus (strain … protein
Translation initiation factor 2 subunit alpha monomer, 10 kDa Saccharolobus solfataricus (strain … protein
transfer RNA monomer, 23 kDa Escherichia coli RNA
Buffer: 10 mM MOPS- NaOH pH 6.7, 200 mM NaCl, 5 mM MgCl 2, 1 mM GDPNP, pH: 6.7
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Nov 26
Medical contrast agents as promising tools for biomacromolecular SAXS experiments. Acta Crystallogr D Struct Biol 78(Pt 9):1120-1130 (2022)
...Thureau A, Mechulam Y, Pérez J, Girard E
RgGuinier 3.6 nm
Dmax 13.0 nm
VolumePorod 102 nm3

SASDQR8 – Exon1 of Non pathogenic form of Huntingtin (H16) with GFP fused at the C-terminus

Huntingtin experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Huntingtin monomer, 39 kDa Homo sapiens protein
Buffer: 20mM BisTris-HCl, 150mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 3
The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity. Nat Struct Mol Biol (2023)
...Thureau A, Allemand F, Swenson RE, Milhiet PE, Crehuet R, Barducci A, Cortés J, Sinnaeve D, Sibille N, Bernadó P
RgGuinier 3.3 nm
Dmax 16.0 nm
VolumePorod 63 nm3

SASDQS8 – Exon1 of Pathogenic form of Huntingtin (H46) with GFP fused at the C-terminus

Huntingtin experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Huntingtin monomer, 43 kDa Homo sapiens protein
Buffer: 20mM BisTris-HCl, 150mM NaCl, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 28
The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity. Nat Struct Mol Biol (2023)
...Thureau A, Allemand F, Swenson RE, Milhiet PE, Crehuet R, Barducci A, Cortés J, Sinnaeve D, Sibille N, Bernadó P
RgGuinier 4.2 nm

SASDRS7 – Radical SAM enzyme peptide epimerase in the presence of SAM (S-adenosyl-L-methionine)

Putative peptide biosynthesis protein YydG experimental SAS data
DADIMODO model
Sample: Putative peptide biosynthesis protein YydG dimer, 80 kDa Bacillus subtilis (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Nov 29
Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme. Nat Chem Biol (2023)
...Thureau A, Legrand P, Berteau O, Benjdia A
RgGuinier 2.9 nm
Dmax 11.8 nm
VolumePorod 115 nm3

SASDNY9 – dark-adapted Orange Carotenoid Protein-wtCtag at 0.7 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.4 nm
Dmax 6.7 nm
VolumePorod 55 nm3

SASDNZ9 – dark-adapted Orange Carotenoid Protein-wtCtag at 3.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 77 nm3

SASDRR7 – Radical SAM enzyme peptide epimerase in the presence of SAM (S-adenosyl-L-methionine) and a peptide subtrate

Putative peptide biosynthesis protein YydG experimental SAS data
DADIMODO model
Sample: Putative peptide biosynthesis protein YydG dimer, 80 kDa Bacillus subtilis (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Nov 30
Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme. Nat Chem Biol (2023)
...Thureau A, Legrand P, Berteau O, Benjdia A
RgGuinier 2.9 nm
Dmax 12.0 nm
VolumePorod 113 nm3

SASDP22 – dark-adapted Orange Carotenoid Protein-wtCtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.8 nm
Dmax 12.7 nm
VolumePorod 85 nm3

SASDP32 – dark-adapted Orange Carotenoid Protien-wtCtag at 23 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.8 nm
Dmax 13.0 nm
VolumePorod 89 nm3

SASDP42 – dark-adapted Orange Carotenoid Protein-R27LNtag at 0.7 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 44 nm3

SASDP52 – dark-adapted Orange Carotenoid Protein-R27LNtag at 3.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 61 nm3

SASDP62 – dark-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 58 nm3

SASDP72 – dark-adapted Orange Carotenoid Protein-R27LNtag at 14 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 58 nm3

SASDP82 – light-adapted Orange Carotenoid Protein-wtCtag at 0.7 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 2.9 nm
Dmax 14.0 nm
VolumePorod 63 nm3

SASDP92 – light-adapted Orange Carotenoid Protein-wtCtag at 3.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 3.4 nm
Dmax 17.0 nm
VolumePorod 78 nm3

SASDPA2 – light-adapted Orange Carotenoid Protein-wtCtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein , 105 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 4.4 nm
Dmax 31.0 nm
VolumePorod 140 nm3

SASDPB2 – light-adapted Orange Carotenoid Protein-R27LNtag at 0.7 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein , 105 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 3.3 nm
Dmax 15.0 nm
VolumePorod 70 nm3

SASDPC2 – light-adapted Orange Carotenoid Protein-R27LNtag at 3.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein , 105 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 3.4 nm
Dmax 19.0 nm
VolumePorod 70 nm3

SASDPD2 – light-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data
DAMMIF model
Sample: Orange carotenoid-binding protein , 105 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 23
Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
...Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP
RgGuinier 4.8 nm
Dmax 26.0 nm
VolumePorod 120 nm3