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18 hits found for Tong

SASDSJ5 – 15mer poly-ADP ribose (PAR15) in 100mM NaCl

15mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 15mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 8 kDa synthetic construct
Buffer: 100 mM NaCl, 20 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Apr 21
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 15(1):7901 (2024)
Wang T, Coshic K, Badiee M, McDonald MR, Aksimentiev A, Pollack L, Leung AKL
RgGuinier 2.4 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDSK5 – 15mer poly-ADP ribose (PAR15) in 100mM NaCl + 1mM MgCl2

15mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 15mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 8 kDa synthetic construct
Buffer: 20 mM Tris-HCl, 1 mM MgCl2, 100 mM NaCl, pH: 7.4
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2022 Apr 21
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 15(1):7901 (2024)
Wang T, Coshic K, Badiee M, McDonald MR, Aksimentiev A, Pollack L, Leung AKL
RgGuinier 2.3 nm
Dmax 9.0 nm
VolumePorod 14 nm3

SASDSL5 – 22mer poly-ADP ribose (PAR22) in 100mM NaCl

22mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 22mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 12 kDa synthetic construct
Buffer: 100 mM NaCl, 20 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Apr 21
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 15(1):7901 (2024)
Wang T, Coshic K, Badiee M, McDonald MR, Aksimentiev A, Pollack L, Leung AKL
RgGuinier 3.3 nm
Dmax 14.0 nm
VolumePorod 32 nm3

SASDSM5 – 22mer poly-ADP ribose (PAR22) in 100mM NaCl + 1mM MgCl2

22mer of adenosine diphosphate ribose (poly-ADP ribose) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: 22mer of adenosine diphosphate ribose (poly-ADP ribose) monomer, 12 kDa synthetic construct
Buffer: 20 mM Tris-HCl, 1 mM MgCl2, 100 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Apr 21
Cation-induced intramolecular coil-to-globule transition in poly(ADP-ribose). Nat Commun 15(1):7901 (2024)
Wang T, Coshic K, Badiee M, McDonald MR, Aksimentiev A, Pollack L, Leung AKL
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 21 nm3

SASDSN5 – A and C rich mixed sequence single stranded RNA in 100mM NaCl

Mixed sequence single stranded RNA, rich in A and C bases experimental SAS data
Mixed sequence single stranded RNA, rich in A and C bases Kratky plot
Sample: Mixed sequence single stranded RNA, rich in A and C bases monomer, 10 kDa synthetic construct RNA
Buffer: 100 mM NaCl, 10 mM MOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at Xenocs BioXolver L with GeniX3D, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Aug 19
Mixed sequence single stranded RNA in 100mM NaCl
Tong Wang
RgGuinier 2.2 nm
Dmax 7.6 nm
VolumePorod 15 nm3

SASDU39 – Nearly full-length monomeric Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8) (7-1110)

Ubiquitin carboxyl-terminal hydrolase 8 experimental SAS data
DAMMIF model
Sample: Ubiquitin carboxyl-terminal hydrolase 8 monomer, 127 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 18
Autoinhibition of ubiquitin-specific protease 8: insights into domain interactions and mechanisms of regulation Journal of Biological Chemistry :107727 (2024)
...Tong Y
RgGuinier 8.4 nm
Dmax 31.8 nm
VolumePorod 327 nm3

SASDQV9 – Nanobody1 in complex with the extracellular domain of human BCMA

Tumor necrosis factor receptor superfamily member 17Nanobody1 experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Nanobody1 Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Nanobody1 monomer, 14 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
Sun Y, Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 3.0 nm
Dmax 9.8 nm
VolumePorod 52 nm3

SASDQW9 – Nanobody2 in complex with the extracellular domain of human BCMA

Tumor necrosis factor receptor superfamily member 17Nanobody2 experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Nanobody2 Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Nanobody2 monomer, 14 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
Sun Y, Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 2.0 nm
Dmax 9.0 nm
VolumePorod 24 nm3

SASDQX9 – Tandem nanobody in complex with the extracellular domain of human BCMA

Tumor necrosis factor receptor superfamily member 17Tandem nanobody experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Tandem nanobody Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Tandem nanobody monomer, 27 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
Sun Y, Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 5.1 nm
Dmax 23.3 nm
VolumePorod 247 nm3

SASDNE8 – apo-Pyrococcus furiosus argonaute protein at 10 °C

Piwi domain-containing protein experimental SAS data
GASBOR model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 142 nm3

SASDPM2 – apo-Pyrococcus furiosus argonaute protein at 25 °C

Piwi domain-containing protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 142 nm3

SASDPN2 – apo-Pyrococcus furiosus argonaute protein at 95 °C

Piwi domain-containing protein experimental SAS data
GASBOR model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.2 nm
Dmax 9.7 nm
VolumePorod 187 nm3

SASDPP2 – apo-Pyrococcus furiosus argonaute protein with urea at 37 °C

Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris-HCl, 250 mM NaCl, 2 mM DTT, 6 M urea, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Nov 17
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 148 nm3

SASDPQ2 – apo-Clostridium butyricum argonaute protein at 10 °C

Clostridium butyricum argonaute protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Clostridium butyricum argonaute protein monomer, 86 kDa protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.2 nm
Dmax 9.7 nm
VolumePorod 188 nm3

SASDPR2 – apo-Clostridium butyricum argonaute protein at 37 °C

Clostridium butyricum argonaute protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Clostridium butyricum argonaute protein monomer, 86 kDa protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.2 nm
Dmax 9.7 nm
VolumePorod 185 nm3

SASDPS2 – apo-Thermus thermophilus argonaute protein at 10 °C

Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 122 nm3

SASDPT2 – apo-Thermus thermophilus argonaute protein at 25 °C

Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 120 nm3

SASDPU2 – apo-Thermus thermophilus argonaute protein at 75 °C

Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
Argonaute protein SAXS investigation
lirong zheng
RgGuinier 3.3 nm
Dmax 9.7 nm
VolumePorod 175 nm3