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20 hits found for Winter

SASDJ43 – Acyl carrier protein-thioesterase domain from Men2

Menisporopsin singlet acyl carrier protein-thioesterase experimental SAS data
Acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: Menisporopsin singlet acyl carrier protein-thioesterase monomer, 45 kDa Menisporopsin theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
SAXS reveals highly flexible interdomain linkers of tandem acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase FEBS Letters (2020)
...Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
RgGuinier 3.6 nm
Dmax 14.4 nm
VolumePorod 75 nm3

SASDJ53 – Doublet acyl carrier protein-thioesterase domain from Men2

Menisporopsin doublet Acyl Carrier Protein-Thioesterase experimental SAS data
Doublet acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: Menisporopsin doublet Acyl Carrier Protein-Thioesterase monomer, 58 kDa Menisporopsis theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
SAXS reveals highly flexible interdomain linkers of tandem acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase FEBS Letters (2020)
...Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
RgGuinier 4.2 nm
Dmax 17.3 nm
VolumePorod 84 nm3

SASDD45 – Type II toxin-antitoxin system HicB family antitoxin - HicB protein

Type II toxin-antitoxin system HicB family antitoxin experimental SAS data
PYMOL model
Sample: Type II toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 28
The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 112 nm3

SASDD55 – Type II toxin-antitoxin system HicB family antitoxin - HicB protein - bound to addiction module toxin, HicA

Type II toxin-antitoxin system HicB family antitoxinAddiction module toxin, HicA experimental SAS data
PYMOL model
Sample: Type II toxin-antitoxin system HicB family antitoxin tetramer, 63 kDa Burkholderia pseudomallei protein
Addiction module toxin, HicA monomer, 7 kDa Burkholderia pseudomallei protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jul 27
The molecular basis of protein toxin HicA-dependent binding of the protein antitoxin HicB to DNA. J Biol Chem (2018)
Winter AJ, Williams C, Isupov MN, Crocker H, Gromova M, Marsh P, Wilkinson OJ, Dillingham MS, Harmer NJ, Titball RW, Crump MP
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 110 nm3

SASDDH6 – Di-domain acyl carrier protein of the seryltransferase from prodigiosin biosynthesis

Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis experimental SAS data
DAMMIF model
Sample: Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis monomer, 22 kDa Serratia sp. ATCC … protein
Buffer: 20 mM Tris supplemented with 5 mM DTT, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2016 Jan 13
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
...Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.4 nm
Dmax 8.4 nm
VolumePorod 39 nm3

SASDSM7 – Full-length Heat shock factor 2-binding protein (HSF2BP)

Heat shock factor 2-binding protein experimental SAS data
Heat shock factor 2-binding protein Kratky plot
Sample: Heat shock factor 2-binding protein tetramer, 150 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl pH 7.5, 250 mM NaCl, and 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Oct 2
BRCA2-HSF2BP oligomeric ring disassembly by BRME1 promotes homologous recombination. Sci Adv 9(43):eadi7352 (2023)
...Winter JM, Ropars V, David G, Arteni AA, Wyman C, Knipscheer P, Kanaar R, Zelensky AN, Zinn-Justin S
RgGuinier 8.5 nm
Dmax 25.0 nm
VolumePorod 364 nm3

SASDKR7 – Hunchback mRNA translation repression complex of Brat-NHL, Pum-HD, Nanos-ZnF and hb NRE2 RNA

Brain tumor proteinMaternal protein pumilioProtein nanoshunchback mRNA Nanos Response Element 2 experimental SAS data
Brain tumor protein Maternal protein pumilio Protein nanos hunchback mRNA Nanos Response Element 2 Kratky plot
Sample: Brain tumor protein monomer, 32 kDa Drosophila melanogaster protein
Maternal protein pumilio monomer, 38 kDa Drosophila melanogaster protein
Protein nanos monomer, 11 kDa Drosophila melanogaster protein
hunchback mRNA Nanos Response Element 2 monomer, 7 kDa Drosophila melanogaster RNA
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 3% glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 12
Structure and dynamics of the quaternary hunchback mRNA translation repression complex. Nucleic Acids Res 49(15):8866-8885 (2021)
...Winter SL, Foot J, Lapouge K, Perez K, Rettel M, Ivanović MT, Masiewicz P, Murciano B, Savitski MM, Loedige I, Hub JS, Gabel F, Hennig J
RgGuinier 3.7 nm
Dmax 12.7 nm
VolumePorod 114 nm3

SASDRF8 – Plasmodium falciparum circumsporozoite protein N-terminal domain at pH 6.6

Circumsporozoite protein experimental SAS data
Circumsporozoite protein Kratky plot
Sample: Circumsporozoite protein monomer, 11 kDa Plasmodium falciparum (isolate … protein
Buffer: 50 mM MES, 500 mM NaCl, 1 mM TCEP, pH: 6.6
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Biophysical characterization of the Plasmodium falciparum circumsporozoite protein's N‐terminal domain Protein Science (2023)
...Winter H, Volkov A, Tompa P, Sterckx Y
RgGuinier 2.3 nm
Dmax 9.1 nm
VolumePorod 24 nm3

SASDR39 – MBP-, SUMO-fused heterodimeric SEPT6-SEPT7 septin coiled coil (MBP-SEPT6c+SUMO-SEPT7c)

Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP)Septin-7 (C-terminal domain of SEPT7 fused to SUMO) experimental SAS data
MULTIFOXS model
Sample: Septin-6 (C-terminal domain of SEPT6 fused to his-tagged MBP) monomer, 55 kDa Homo sapiens protein
Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
...Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.4 nm
Dmax 21.5 nm
VolumePorod 142 nm3

SASDJ49 – Apo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Apo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
...Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.9 nm
Dmax 9.9 nm
VolumePorod 83 nm3

SASDR49 – MBP-, SUMO-fused heterodimeric SEPT8-SEPT7 septin coiled coil (MBP-SEPT8c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-8 (C-terminal domain of SEPT8 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-8 (C-terminal domain of SEPT8 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
...Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.3 nm
Dmax 19.9 nm
VolumePorod 140 nm3

SASDJ59 – Holo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Holo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
...Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 72 nm3

SASDR59 – MBP-, SUMO-fused heterodimeric SEPT10-SEPT7 septin coiled coil (MBP-SEPT10c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-10 (C-terminal domain of SEPT10 fused to MBP) experimental SAS data
DAMMIN model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-10 (C-terminal domain of SEPT10 fused to MBP) monomer, 56 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
...Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.7 nm
Dmax 21.7 nm
VolumePorod 154 nm3

SASDR69 – MBP-, SUMO-fused heterodimeric SEPT11-SEPT7 septin coiled coil (MBP-SEPT11c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-11 (C-terminal domain of SEPT11 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-11 (C-terminal domain of SEPT11 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
...Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.2 nm
Dmax 20.9 nm
VolumePorod 139 nm3

SASDR79 – MBP-, SUMO-fused heterodimeric SEPT14-SEPT7 septin coiled coil (MBP-SEPT14c+SUMO-SEPT7c)

Septin-7 (C-terminal domain of SEPT7 fused to SUMO)Septin-14 (C-terminal domain of SEPT14 fused to MBP) experimental SAS data
MULTIFOXS model
Sample: Septin-7 (C-terminal domain of SEPT7 fused to SUMO) monomer, 25 kDa Homo sapiens protein
Septin-14 (C-terminal domain of SEPT14 fused to MBP) monomer, 55 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 3
X-ray structure of the metastable SEPT14-SEPT7 coiled coil reveals a hendecad region crucial for heterodimerization. Acta Crystallogr D Struct Biol (2023)
...Winter AJ, D'Muniz Pereira H, Woolfson DN, Crump MP, Garratt RC
RgGuinier 5.1 nm
Dmax 20.3 nm
VolumePorod 143 nm3

SASDB99 – Extracellular assembly of the human TSLP:TSLPR:IL-7Ralpha complex

Thymic stromal lymphopoietinInterleukin-7 receptor subunit alphaCytokine receptor-like factor 2 experimental SAS data
ALLOSMOD model
Sample: Thymic stromal lymphopoietin monomer, 15 kDa Homo sapiens protein
Interleukin-7 receptor subunit alpha monomer, 26 kDa Homo sapiens protein
Cytokine receptor-like factor 2 monomer, 24 kDa Homo sapiens protein
Buffer: 10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 8
Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun 8:14937 (2017)
...Winter H, Beyaert R, Lippens G, Savvides SN
RgGuinier 3.1 nm
Dmax 10.3 nm
VolumePorod 98 nm3

SASDH79 – Human Cation-independent mannose-6-phosphate receptor domains 9-10

Cation-independent mannose-6-phosphate receptor experimental SAS data
MULTIFOXS model
Sample: Cation-independent mannose-6-phosphate receptor dimer, 67 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 3.3 nm
Dmax 9.2 nm
VolumePorod 93 nm3

SASDH59 – Human Cation-independent mannose-6-phosphate receptor domains 9-10 wih mannose-6-phosphate

Cation-independent mannose-6-phosphate receptor experimental SAS data
OTHER model
Sample: Cation-independent mannose-6-phosphate receptor dimer, 67 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 3.3 nm
Dmax 9.1 nm
VolumePorod 88 nm3

SASDH69 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated

Cation-independent mannose-6-phosphate receptor experimental SAS data
OTHER model
Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 48 nm3

SASDJ23 – Human Cation-independent mannose-6-phosphate receptor domains 9-10: Deglycosylated with mannose-6-phosphate

Cation-independent mannose-6-phosphate receptor experimental SAS data
MULTIFOXS model
Sample: Cation-independent mannose-6-phosphate receptor monomer, 34 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
Structure of the Human Cation-Independent Mannose 6-Phosphate/IGF2 Receptor Domains 7–11 Uncovers the Mannose 6-Phosphate Binding Site of Domain 9 Structure (2020)
...Winter A, Nicholls R, Harlos K, Jones E, Berger I, Hassan A, Crump M
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 69 nm3