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9 hits found for Acyl carrier protein

SASDJ43 – Acyl carrier protein-thioesterase domain from Men2

Menisporopsin singlet acyl carrier protein-thioesterase experimental SAS data
Acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: ...acyl carrier protein-thioesterase monomer, 45 kDa Menisporopsin theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
...acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase FEBS Letters (2020)
Bunnak W, Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
RgGuinier 3.6 nm
Dmax 14.4 nm
VolumePorod 75 nm3

SASDJ53 – ...acyl carrier protein-thioesterase domain from Men2

Menisporopsin doublet Acyl Carrier Protein-Thioesterase experimental SAS data
Doublet acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: ...Acyl Carrier Protein-Thioesterase monomer, 58 kDa Menisporopsis theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
...acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase FEBS Letters (2020)
Bunnak W, Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
RgGuinier 4.2 nm
Dmax 17.3 nm
VolumePorod 84 nm3

SASDDB3 – Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
...protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 204 nm3

SASDDC3 – Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier proteinIron-sulfur cluster assembly enzyme ISCU, mitochondrial experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
...protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 218 nm3

SASDDD3 – Human mitochondrial cysteine desulfurase-ISCU-Frataxin (NFS1, ISD11 and Acp heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier proteinIron-sulfur cluster assembly enzyme ISCU, mitochondrialFrataxin, mitochondrial experimental SAS data
HADDOCK model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
...protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein
Frataxin, mitochondrial dimer, 29 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 Apr 18
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 287 nm3

SASDDH6 – ...acyl carrier protein of the seryltransferase from prodigiosin biosynthesis

Di-domain acyl carrier protein of PigH from prodigiosin biosynthesis experimental SAS data
DAMMIF model
Sample: ...acyl carrier protein of PigH from prodigiosin biosynthesis monomer, 22 kDa Serratia sp. ATCC … protein
Buffer: 20 mM Tris supplemented with 5 mM DTT, pH: 7
Experiment: SAXS data collected at BL1.3W, Synchrotron Light Research Institute (SLRI) on 2016 Jan 13
...Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.4 nm
Dmax 8.4 nm
VolumePorod 39 nm3

SASDJ49 – Apo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Apo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
...Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.9 nm
Dmax 9.9 nm
VolumePorod 83 nm3

SASDJ59 – Holo PigH ACP1-ACP2

4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH experimental SAS data
Holo PigH ACP1-ACP2 Rg histogram
Sample: 4-hydroxy-2,2'-bipyrrole-5-methanol synthase PigH monomer, 22 kDa Serratia sp. protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Mar 3
...Acyl Carrier Protein from Prodigiosin Biosynthesis. Biochemistry (2021)
Thongkawphueak T, Winter AJ, Williams C, Maple HJ, Soontaranon S, Kaewhan C, Campopiano DJ, Crump MP, Wattana-Amorn P
RgGuinier 2.8 nm
Dmax 9.7 nm
VolumePorod 72 nm3

SASDLR4 – ...acyl-[acyl-carrier-protein] reductase, FabG

3-oxoacyl-[acyl-carrier-protein] reductase FabG experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...acyl-[acyl-carrier-protein] reductase FabG , 26 kDa Escherichia coli (strain … protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1
Protein quaternary structures in solution are a mixture of multiple forms Chemical Science 13(39):11680-11695 (2022)
Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G
RgGuinier 3.6 nm
Dmax 10.6 nm
VolumePorod 212 nm3